Exogenous ligand binding to the [Fe4S4] cluster in Pyrococcus furiosus ferredoxin

1991 ◽  
Vol 113 (7) ◽  
pp. 2799-2800 ◽  
Author(s):  
Richard C. Conover ◽  
Jae Bum Park ◽  
Michael W. W. Adams ◽  
Michael K. Johnson
Keyword(s):  
Ligand Binding ◽  
Pyrococcus Furiosus ◽  
Exogenous Ligand

scholarly journals Crystal Structure, Exogenous Ligand Binding, and Redox Properties of an Engineered Diiron Active Site in a Bacterial Hemerythrin

2013 ◽  
Vol 52 (22) ◽  
pp. 13014-13020 ◽  
Author(s):  
Yasunori Okamoto ◽  
Akira Onoda ◽  
Hiroshi Sugimoto ◽  
Yu Takano ◽  
Shun Hirota ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Ligand Binding ◽  
Active Site ◽  
Redox Properties ◽  
Exogenous Ligand

scholarly journals Role of the membrane cholesterol-glycosphingolipid complex as a ‘transistor’ to regulate GSL receptor function and signaling of both lipids

2017 ◽  
Author(s):  
Anton Novak ◽  
Clifford Lingwood
Keyword(s):  
Ligand Binding ◽  
Lipid Rafts ◽  
Clinical Importance ◽  
Membrane Lipid ◽  
Signal Pathways ◽  
Membrane Cholesterol ◽  
Plasma Membrane Lipid ◽  
Dependent Signal ◽  
Partial Dissociation ◽  
Exogenous Ligand

AbstractCholesterol and glycosphingolipids (GSL) are the major species that accumulate in plasma membrane lipid rafts. These complexes imbue the membrane with increased order, which in turn, plays a central role in the transmembrane signaling foci lipid rafts provide. In addition, both GSL and cholesterol binding can mediate (separate) signal pathways. We have shown that cholesterol and GSLs however, form a complex in which the GSL sugar is reoriented from a membrane perpendicular to parallel format, becoming largely unavailable for exogenous ligand binding. Similarly, the steroid hydroxyl is masked, restricting access of cholesterol ligands. This was observed in model and cell membranes and in human tumour frozen tissue sections. We now show the order of exogenous ligand binding plays a significant role to determine the extent of GSL or cholesterol receptor activity. Ligand binding to cholesterol enhances subsequent GSL recognition and vice versa, suggesting that ligand binding to “free” receptor (membrane perpendicular GSL carbohydrate, nonmasked cholesterol) can result in partial dissociation of the GSL/cholesterol complex to allow additional GSL ligand and cholesterol ligand binding. Since many GSLs can complex with membrane cholesterol, the binding of a single cholesterol ligand may unmask cholesterol-complexed GSL for increased binding of both a single or multiple GSL-specific ligands. We show that multiple cholesterol-masked GSLs can be coincident in tissues. This provides a mechanism for GSL-dependent signal amplification and diversification, representing a biological ‘transistor’, regulating amplitude and potentially, diversity of GSL signaling. The process represents a new mechanism of ‘cross-talk’ between GSL and cholesterol signaling. This is of clinical importance since we have found cholesterol/GSL masking applies to monoclonal anti GSL antibodies in development and in current use as antineoplastic therapeutics.

Accessibility to the active site of methane monooxygenase: the first demonstration of exogenous ligand binding to the diiron cluster

1992 ◽  
Vol 114 (22) ◽  
pp. 8711-8713 ◽  
Author(s):  
Kristoffer K. Andersson ◽  
Timothy E. Elgren ◽  
Lawrence Que ◽  
John D. Lipscomb
Keyword(s):  
Ligand Binding ◽  
Active Site ◽  
Methane Monooxygenase ◽  
Exogenous Ligand

scholarly journals 1P162 Exogenous Ligand Binding Mechanism of Cytoglobin(5. Heme protein,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

2006 ◽  
Vol 46 (supplement2) ◽  
pp. S187
Author(s):  
Tadayuki Uno ◽  
Kaori Kukino ◽  
Hatsuo Maeda ◽  
Yoshikazu Tomisugi ◽  
Yoshinobu Ishikawa ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Poster Session ◽  
Heme Protein ◽  
Binding Mechanism ◽  
Meeting Program ◽  
Exogenous Ligand

scholarly journals 2P068 Exogenous ligand binding properties of human neuroglobin

2004 ◽  
Vol 44 (supplement) ◽  
pp. S126
Author(s):  
T. Uno ◽  
H. Tsutsumi ◽  
D. Ryu ◽  
K. Kiyota ◽  
Y. Tomisugi ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Binding Properties ◽  
Exogenous Ligand

Electronic, Magnetic, Redox, and Ligand-Binding Properties of [MFe3S4] Clusters (M = Zn, Co, Mn) in Pyrococcus furiosus Ferredoxin

1995 ◽  
Vol 34 (21) ◽  
pp. 5358-5369 ◽  
Author(s):  
Michael G. Finnegan ◽  
Richard C. Conover ◽  
Jae-Bum Park ◽  
Zhi Hao Zhou ◽  
Michael W. W. Adams ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Pyrococcus Furiosus ◽  
Binding Properties
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