Studies of model bile solutions using surfactant ion electrodes

1983 ◽  
Vol 87 (24) ◽  
pp. 5015-5019 ◽  
Author(s):  
Kyoo Ryu ◽  
James M. Lowery ◽  
D. Fennell Evans ◽  
E. L. Cussler
Keyword(s):  
Model Bile

scholarly journals Use of 1H-NMR to determine the distribution of lecithin between the micellar and vesicular phases in model bile.

1990 ◽  
Vol 31 (7) ◽  
pp. 1315-1321
Author(s):  
AK Groen ◽  
BG Goldhoorn ◽  
PH Egbers ◽  
RA Chamuleau ◽  
GN Tytgat ◽  
...  
Keyword(s):  
1H Nmr ◽  
Model Bile

scholarly journals Effect of cholesterol nucleation-promoting activity on cholesterol solubilization in model bile.

1989 ◽  
Vol 30 (1) ◽  
pp. 51-58
Author(s):  
A K Groen ◽  
R Ottenhoff ◽  
P L Jansen ◽  
J van Marle ◽  
G N Tytgat
Keyword(s):  
Cholesterol Solubilization ◽  
Model Bile

The Nucleation of Cholesterol Monohydrate Crystals in Model Bile Solutions

Gallstones ◽  
1979 ◽  
pp. 169-181 ◽  
Author(s):  
E. W. Toor ◽  
D. F. Evans ◽  
E. L. Cussler
Keyword(s):  
Cholesterol Monohydrate ◽  
Model Bile

Influence of cholesterol crystallization effector proteins on vesicle fusion in supersaturated model bile

1999 ◽  
Vol 14 (7) ◽  
pp. 669-674 ◽  
Author(s):  
Yoshihiro Hattori ◽  
Susumu Tazuma ◽  
Gunji Yamashita ◽  
Goro Kajiyama
Keyword(s):  
Vesicle Fusion ◽  
Effector Proteins ◽  
Model Bile

scholarly journals Lecithin hydrophobicity modulates the process of cholesterol crystal nucleation and growth in supersaturated model bile systems

1996 ◽  
Vol 318 (1) ◽  
pp. 139-144 ◽  
Author(s):  
Hidenori OCHI ◽  
Susumu TAZUMA ◽  
Goro KAJIYAMA
Keyword(s):  
Acyl Chain ◽  
Egg Yolk ◽  
Nucleation And Growth ◽  
Particle Size Analysis ◽  
Crystal Nucleation ◽  
Size Analysis ◽  
Cholesterol Crystal ◽  
Egg Yolk Phosphatidylcholine ◽  
Bile Cholesterol ◽  
Model Bile

The present study was performed to determine whether the degree of lecithin hydrophobicity regulates bile metastability and, therefore, affects the process of cholesterol crystallization. Supersaturated model bile (MB) solutions were prepared with an identical composition on a molar basis (taurocholate/lecithin/cholesterol, 73:19.5:7.5; total lipid concentration 9 g/dl) except for the lecithin species; egg yolk phosphatidylcholine, soybean phosphatidylcholine, 1-palmitoyl-2-linoleoyl-sn-phosphatidylcholine, dilinoleoyl phosphatidylcholine and dipalmitoyl phosphatidylcholine. Each MB solution was incubated and sequentially examined. Video-enhanced contrast microscopy demonstrated that the rate of vesicular aggregation and fusion correlated with the degree of lecithin hydrophobicity, and that the rate of cholesterol crystal nucleation correlated with the degree of lecithin hydrophilicity. In MBs containing less hydrophobic lecithin, needle-like crystals developed and transformed into mature plate-like crystals, whereas classical plate-like crystals were consistently observed in MBs composed of hydrophobic lecithin. Laser-diffraction particle size analysis demonstrated that the increase in lecithin hydrophobicity enlarged the vesicle dimension, enhancing its cholesterol-holding capacity. Correlation between vesicular cholesterol packing density and lecithin hydrophobicity suggests that the process of bile cholesterol nucleation and growth is regulated, in part, by acyl chain unsaturation in lecithin. Since the composition of biliary lecithins is responsive to dietary manipulations, this study provides new insights into the prevention of cholesterol gallstones.

Cholesterol crystallization from a dilute bile salt-rich model bile

1994 ◽  
Vol 144 (1-2) ◽  
pp. 79-86 ◽  
Author(s):  
Fred M. Konikoff ◽  
Martin C. Carey
Keyword(s):  
Bile Salt ◽  
Model Bile

Heterogeneity of Human Biliary Mucin: Functional Implications

1994 ◽  
Vol 86 (1) ◽  
pp. 75-82 ◽  
Author(s):  
J. Henriëtte Klinkspoor ◽  
Michel J. A. van Wijland ◽  
Carolien A. M. Koeleman ◽  
Willem van Dijk ◽  
Guido N. J. Tytgat ◽  
...  
Keyword(s):  
Cholesterol Gallstone ◽  
Lectin Binding ◽  
Gallstone Disease ◽  
Functional Characterization ◽  
The Other ◽  
Binding Studies ◽  
Human Gallbladder ◽  
Patient Heterogeneity ◽  
Model Bile

1. Human gallbladder mucin has been implicated as playing a role in the pathogenesis of gallstones. In previous studies no differences have been found in the content or composition of mucins derived from control bile or cholesterol gallstone bile. Until now, no differences were also found between these two groups of mucins with regard to their ability to cause cholesterol nucleation. In the accompanying paper we have reported that there is a strong heterogeneity of gallbladder mucins derived from individual patients (M. J. A. van Wijland, J. H. Klinkspoor, L. Th. de Wit, R. P. J. Oude Elferink, G. N. J. Tytgat and A. K. Groen, Clin Sci 1994; 86: 67–74). In the present study we further investigated a possible patient to patient heterogeneity of mucin by means of immunological and functional characterization of mucins isolated from hepatic bile of six different patients with gallstones. 2. Considerable heterogeneity was found. Two of the mucins barely reacted with a polyclonal anti-mucin antibody, whereas the other four mucins reacted very strongly. Lectin-binding studies indicated that the glycans of these two mucins expressed less D-N-acetylgalactosamine residues than the other four mucins. This was confirmed by analyses of the glycan compositions. These studies furthermore indicated that the glycans were of the O-linked type, contained α-D-N-acetylglucosamine and were fucosylated, sialy-lated and sulphated to different extents. Except for a strong heterogeneity in the sugar composition of the mucins, heterogeneity was also found in the biological activity of the mucins. The two immunologically diverging mucins nucleated cholesterol from model bile 1–2 days earlier and also caused an almost threefold more rapid rupture of cholesterol/phospholipid vesicles than the other mucins. 3. We conclude that considerable differences exist between mucins derived from individual patients and that the heterogeneity in glycan composition might play a role in the pathogenesis of gallstone disease.

Sign in / Sign up

Export Citation Format

Share Document