scholarly journals The Osmotic Concentration and Electrical Conductivity of the Tissue Fluids of Ligneous and Herbaceous Plants

1921 ◽  
Vol 25 (2) ◽  
pp. 122-146 ◽  
Author(s):  
J. A. Harris ◽  
R. A. Gortner ◽  
J. V. Lawrence
Keyword(s):  
Electrical Conductivity ◽  
Herbaceous Plants ◽  
Osmotic Concentration ◽  
Tissue Fluids

Maximum values of osmotic concentration in plant tissue fluids

1921 ◽  
Vol 18 (4) ◽  
pp. 106-109 ◽  
Author(s):  
J. A. Harris ◽  
R. A. Gortner ◽  
W. F. Hofmann ◽  
A. T. Valentine
Keyword(s):  
Plant Tissue ◽  
Osmotic Concentration ◽  
Tissue Fluids

Tomato plant growth as affected by horizontally unequal osmotic concentrations in rock-wool.

1981 ◽  
Vol 29 (3) ◽  
pp. 189-197
Author(s):  
A. Cerda ◽  
J.P.N.L.R. van Eysinga
Keyword(s):  
Electrical Conductivity ◽  
Plant Growth ◽  
Root Zone ◽  
Nutrient Concentrations ◽  
Osmotic Concentration ◽  
Tomato Plants ◽  
Split Root ◽  
Split Root System ◽  
The Mean ◽  
Rock Wool

Tomato plants, cv. Moneydor, were grown in rockwool in a split-root system with equal or different osmotic concentrations. Fruit yield was negatively correlated with the mean electrical conductivity (EC) of both parts of the system. In treatments with two different EC values in the root zone, root development was better in the part with the low EC, and water uptake was higher. Nutrient concentrations showed an increase in the part with the low EC when differences in EC between both parts were 4 mS/cm [4 mmho/cm] or more. A possible explanation is that solutes move through the roots from the part with high to the part with the low osmotic concentration. (Abstract retrieved from CAB Abstracts by CABI’s permission)

Removing Substrate Background in TEM Microanalysis

1974 ◽  
Vol 32 ◽  
pp. 568-569
Author(s):  
John C. Russ ◽  
Nicholas C. Barbi
Keyword(s):  
Electrical Conductivity ◽  
Rapid Growth ◽  
Organic Film ◽  
Absolute Concentration ◽  
Background Signal ◽  
X Ray ◽  
Elemental Concentrations ◽  
Transmission Electron ◽  
Electron Microscopes ◽  
The Continuum

The rapid growth of interest in attaching energy-dispersive x-ray analysis systems to transmission electron microscopes has centered largely on microanalysis of biological specimens. These are frequently either embedded in plastic or supported by an organic film, which is of great importance as regards stability under the beam since it provides thermal and electrical conductivity from the specimen to the grid.Unfortunately, the supporting medium also produces continuum x-radiation or Bremsstrahlung, which is added to the x-ray spectrum from the sample. It is not difficult to separate the characteristic peaks from the elements in the specimen from the total continuum background, but sometimes it is also necessary to separate the continuum due to the sample from that due to the support. For instance, it is possible to compute relative elemental concentrations in the sample, without standards, based on the relative net characteristic elemental intensities without regard to background; but to calculate absolute concentration, it is necessary to use the background signal itself as a measure of the total excited specimen mass.

Macromolecular structures of biological specimens are not obscured by controlled osmium impregnation

1983 ◽  
Vol 41 ◽  
pp. 606-607
Author(s):  
Klaus-Ruediger Peters ◽  
Samuel A. Green
Keyword(s):  
Thin Films ◽  
Electrical Conductivity ◽  
Critical Point ◽  
Metal Films ◽  
High Magnification ◽  
Osmium Impregnation ◽  
Instrumental Resolution ◽  
20 Nm ◽  
Continuous Metal

High magnification imaging of macromolecules on metal coated biological specimens is limited only by wet preparation procedures since recently obtained instrumental resolution allows visualization of topographic structures as smal l as 1-2 nm. Details of such dimensions may be visualized if continuous metal films with a thickness of 2 nm or less are applied. Such thin films give sufficient contrast in TEM as well as in SEM (SE-I image mode). The requisite increase in electrical conductivity for SEM of biological specimens is achieved through the use of ligand mediated wet osmiuum impregnation of the specimen before critical point (CP) drying. A commonly used ligand is thiocarbohvdrazide (TCH), first introduced to TEM for en block staining of lipids and glvcomacromolecules with osmium black. Now TCH is also used for SEM. However, after ligand mediated osinification nonspecific osmium black precipitates were often found obscuring surface details with large diffuse aggregates or with dense particular deposits, 2-20 nm in size. Thus, only low magnification work was considered possible after TCH appl ication.

Sign in / Sign up

Export Citation Format

Share Document