scholarly journals The Resistance of Glass Tubing to Bursting Pressure

1904 ◽  
Vol 8 (1) ◽  
pp. 37-55 ◽  
Author(s):  
W. P. Bradley ◽  
A. W. Browne
Keyword(s):  
Bursting Pressure ◽  
Glass Tubing

scholarly journals Ileocolonic Healing After Extended Small Bowel Resection in Mice: NOD2 Deficiency Impairs Anastomotic Healing and Postoperative Outcome

2021 ◽  
Author(s):  
Maria Witte ◽  
Johannes Reiner ◽  
Karen Bannert ◽  
Robert Jaster ◽  
Christian Maschmeier ◽  
...  
Keyword(s):  
Gene Expression ◽  
Crohn Disease ◽  
Transforming Growth Factor ◽  
Bowel Resection ◽  
Anastomotic Healing ◽  
Matrix Metalloproteinase 2 ◽  
Ileocecal Resection ◽  
Bursting Pressure ◽  
Septic Complications ◽  
Gelatinase Activity

Abstract Background Nucleotide-binding oligomerization domain-containing protein 2 (NOD2) mutations are a genetic risk factor for Crohn disease. Ileocecal resection is the most often performed surgery in Crohn disease. We investigated the effect of Nod2 knockout (KO) status on anastomotic healing after extended ileocecal resection (ICR) in mice. Methods Male C57BL6/J wild-type and Nod2 KO mice underwent an 11 cm resection of the terminal ileum including the cecum. An end-to-end jejuno-colostomy was performed. Animals were killed after 5 days investigating bursting pressure, hydroxyproline content, and expression of matrix metabolism genes, key cytokines, and histology of the anastomosis. Results Mortality was higher in the Nod2 KO group but not because of local or septic complications. Bursting pressure was significantly reduced in the Nod2 KO mice (32.5 vs 78.0 mmHg, P < 0.0024), whereas hydroxyprolin content was equal. The amount of granulation tissue at the anastomosis was similar but more unstructured in the Nod2 KO mice. Gene expression measured by real-time polymerase chain reaction showed significantly increased expression for Collagen 1alpha and for collagen degradation as measured by matrix metalloproteinase-2, -9, and -13 in the Nod2 KO mice. Gelatinase activity from anastomotic tissue was enhanced by Nod2 status. Gene expression of arginase I, tumor necrosis factor-α, and transforming growth factor-ß but not inducible nitric oxide synthase were also increased at the anastomosis in the Nod2 KO mice compared with the control mice. Conclusions We found that Nod2 deficiency results in significantly reduced bursting pressure after ileocecal resection. This effect is mediated via an increased matrix turnover. Patients with genetic NOD2 variations may be prone to anastomotic failure after bowel resection.

The Use of Glass Tubing for Pulleys

1935 ◽  
Vol 47 (1) ◽  
pp. 157
Author(s):  
Wm. D. Turner
Keyword(s):  
Glass Tubing

Experimental Evaluation of Bursting Pressure in Lymphatic Vessels with Ultrasonically Activated Shears

2004 ◽  
Vol 29 (1) ◽  
pp. 106-109 ◽  
Author(s):  
Kaoru Abe ◽  
Masanori Terashima ◽  
Hisataka Fujiwara ◽  
Akinori Takagane ◽  
Noriyuki Uesugi ◽  
...  
Keyword(s):  
Experimental Evaluation ◽  
Lymphatic Vessels ◽  
Bursting Pressure

Failure of Glass Tubing Vials during Lyophilization

2018 ◽  
Vol 73 (1) ◽  
pp. 30-38 ◽  
Author(s):  
David R. Machak ◽  
Gary L. Smay
Keyword(s):  
Glass Tubing

scholarly journals Experimental Study of Mechanical Integrity Testing in Stapled Large Bowel: Methylene Blue Leak Test Is Not Inferior to Air Leak Test

2020 ◽  
pp. 1-9
Author(s):  
Marius Kryzauskas ◽  
Austeja Elzbieta Degutyte ◽  
Vilius Abeciunas ◽  
Beatrice Lukenaite ◽  
Eugenijus Jasiunas ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Colorectal Anastomosis ◽  
Linear Stapler ◽  
Bursting Pressure ◽  
Air Leak ◽  
Leak Test ◽  
Pressure Monitor ◽  
Mechanical Integrity ◽  
Air Leak Test ◽  
Significant Difference

<b><i>Background/Objectives:</i></b> Anastomotic leakage remains the most devastating postoperative complication in colorectal surgery. The mechanical integrity of the newly formed colorectal anastomosis can be evaluated by visual inspection intraoperatively; both air leak and liquid leak tests are also used to evaluate the integrity of stapled colorectal anastomoses. It is not clear whether double-stapled anastomoses are more prone to leaks than single-stapled anastomoses. The aim of our study was to compare the methylene blue and the air leak test in the experimental setting of single-stapled and double-stapled porcine bowels. <b><i>Methods:</i></b> Twenty-four distal colons were excised from slaughtered pigs without delay. The proximal bowel end was closed with a linear stapler using blue cartridges. The bowels were randomly divided into single-stapled or double-stapled groups. Air leak and methylene blue leak tests were performed. A digital pressure monitor with a gradual pressure increase function was used to both gradually increase pressure within the bowel and to determine the pressure at which the stapler line disintegrated. <b><i>Results:</i></b> Air leakage occurred at a mean pressure of 51.62 (±16.60) mm Hg and methylene blue leakage occurred at 46.54 (±16.78) mm Hg (<i>p</i> = 0.31). The air and methylene blue leaks occurred at comparable pressures in single-stapled bowels and in double-stapled bowels (47.21 [±14.02] mm Hg vs. 50.96 [±19.15] mm Hg, <i>p</i> = 0.6). <b><i>Conclusions:</i></b> The methylene blue solution leak test is not inferior to the air leak test. There is no significant difference in bursting pressure between single-stapled and double-stapled anastomoses.

Sign in / Sign up

Export Citation Format

Share Document