Free-radical intermediates in the reaction of the hydroxyl radical with amino acids

1968 ◽  
Vol 72 (6) ◽  
pp. 1926-1931 ◽  
Author(s):  
Hitoshi Taniguchi ◽  
Katsuji Fukui ◽  
Shunichi Ohnishi ◽  
Hiroyuki Hatano ◽  
Hideo Hasegawa ◽  
...  
Keyword(s):  
Amino Acids ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Radical Intermediates

Free-radical intermediates in the reaction of the hydroxyl radical with amino acid derivatives and related compounds

1970 ◽  
Vol 74 (16) ◽  
pp. 3063-3065 ◽  
Author(s):  
Hitoshi Taniguchi ◽  
Hiroyuki Hatano ◽  
Hideo Hasegawa ◽  
Tetsuo Maruyama
Keyword(s):  
Free Radical ◽  
Amino Acid ◽  
Hydroxyl Radical ◽  
Amino Acid Derivatives ◽  
Radical Intermediates ◽  
Related Compounds

Free-radical intermediates in the reaction of the hydroxyl radical with dialkyl sulfoxides

1972 ◽  
Vol 76 (1) ◽  
pp. 135-138 ◽  
Author(s):  
Hitoshi Taniguchi ◽  
Hideo Takagi ◽  
Hiroyuki Hatano
Keyword(s):  
Free Radical ◽  
Hydroxyl Radical ◽  
Radical Intermediates

Hydroxyl radical induced decarboxylation of amino acids. Decarboxylation vs bond formation in radical intermediates

1991 ◽  
Vol 113 (6) ◽  
pp. 2141-2145 ◽  
Author(s):  
L. Kraig Steffen ◽  
Richard S. Glass ◽  
Mahmood Sabahi ◽  
George S. Wilson ◽  
Christian Schoeneich ◽  
...  
Keyword(s):  
Amino Acids ◽  
Hydroxyl Radical ◽  
Bond Formation ◽  
Radical Intermediates

scholarly journals Multiplatform Physiologic and Metabolic Phenotyping Reveals Microbial Toxicity

mSystems ◽  
2018 ◽  
Vol 3 (6) ◽  
Author(s):  
Jingwei Cai ◽  
Robert G. Nichols ◽  
Imhoi Koo ◽  
Zachary A. Kalikow ◽  
Limin Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Flow Cytometry ◽  
Free Radical ◽  
Gut Microbiota ◽  
Structure And Function ◽  
Metabolic Phenotyping ◽  
And Function

ABSTRACTThe gut microbiota is susceptible to modulation by environmental stimuli and therefore can serve as a biological sensor. Recent evidence suggests that xenobiotics can disrupt the interaction between the microbiota and host. Here, we describe an approach that combinesin vitromicrobial incubation (isolated cecal contents from mice), flow cytometry, and mass spectrometry- and1H nuclear magnetic resonance (NMR)-based metabolomics to evaluate xenobiotic-induced microbial toxicity. Tempol, a stabilized free radical scavenger known to remodel the microbial community structure and functionin vivo, was studied to assess its direct effect on the gut microbiota. The microbiota was isolated from mouse cecum and was exposed to tempol for 4 h under strict anaerobic conditions. The flow cytometry data suggested that short-term tempol exposure to the microbiota is associated with disrupted membrane physiology as well as compromised metabolic activity. Mass spectrometry and NMR metabolomics revealed that tempol exposure significantly disrupted microbial metabolic activity, specifically indicated by changes in short-chain fatty acids, branched-chain amino acids, amino acids, nucleotides, glucose, and oligosaccharides. In addition, a mouse study with tempol (5 days gavage) showed similar microbial physiologic and metabolic changes, indicating that thein vitroapproach reflectedin vivoconditions. Our results, through evaluation of microbial viability, physiology, and metabolism and a comparison ofin vitroandin vivoexposures with tempol, suggest that physiologic and metabolic phenotyping can provide unique insight into gut microbiota toxicity.IMPORTANCEThe gut microbiota is modulated physiologically, compositionally, and metabolically by xenobiotics, potentially causing metabolic consequences to the host. We recently reported that tempol, a stabilized free radical nitroxide, can exert beneficial effects on the host through modulation of the microbiome community structure and function. Here, we investigated a multiplatform phenotyping approach that combines high-throughput global metabolomics with flow cytometry to evaluate the direct effect of tempol on the microbiota. This approach may be useful in deciphering how other xenobiotics directly influence the microbiota.

Sign in / Sign up

Export Citation Format

Share Document