Experimental Investigations on the Light Scattering of Colloidal Spheres. VII. Resolving Power of the σ-Spectra Method for Determining Size Distribution Curves1

1964 ◽  
Vol 68 (4) ◽  
pp. 931-934 ◽  
Author(s):  
Wilfried Heller ◽  
Morton L. Wallach
Keyword(s):  
Light Scattering ◽  
Size Distribution ◽  
Resolving Power ◽  
Experimental Investigations ◽  
Colloidal Spheres

scholarly journals Light-scattering studies on deoxyribonucleic acid flexibility. The solution properties of a small circular deoxyribonucleic acid molecule

1972 ◽  
Vol 130 (4) ◽  
pp. 1019-1028 ◽  
Author(s):  
Douglas J. Jolly ◽  
Ailsa M. Campbell
Keyword(s):  
Light Scattering ◽  
Deoxyribonucleic Acid ◽  
Persistence Length ◽  
Resolving Power ◽  
Experimental Investigations ◽  
Volume Factor ◽  
Circular Dna ◽  
Dna Molecule ◽  
Double Stranded Dna ◽  
Infected Cells

Previous investigations on the persistence length of DNA in solution have revealed large discrepancies between hydrodynamic results and those from light-scattering techniques which have potentially a greater resolving power. The information obtained from experiments on a small circular DNA molecule has resolved these discrepancies. The non-superhelical circular double-stranded DNA molecule from bacteriophage [unk]X174-infected cells is small enough to permit accurate light-scattering extrapolations, and its solutions have negligible anisotropy. The persistence length obtained from experimental investigations on this molecule is comparable with that obtained by hydrodynamic techniques, even with variation of the excluded-volume factor.

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