Meaning of diffusion-controlled association rate constants in enzymology

1979 ◽  
Vol 83 (20) ◽  
pp. 2662-2665 ◽  
Author(s):  
H. Nakatani ◽  
H. B. Dunford
Keyword(s):  
Rate Constants ◽  
Association Rate ◽  
Diffusion Controlled ◽  
Association Rate Constants

scholarly journals Effect of heparin on the glia-derived-nexin-thrombin interaction

1989 ◽  
Vol 257 (1) ◽  
pp. 191-196 ◽  
Author(s):  
A Wallace ◽  
G Rovelli ◽  
J Hofsteenge ◽  
S R Stone
Keyword(s):  
Rate Constants ◽  
Antithrombin Iii ◽  
Kinetic Properties ◽  
Association Rate ◽  
High Affinity ◽  
Diffusion Controlled ◽  
Different Types ◽  
Human Thrombin ◽  
Alpha Beta ◽  
Association Rate Constants

In order to determine the specificity of the interaction between thrombin and glia-derived nexin (GdN), the inactivation of proteolytically modified human thrombin species by GdN has been studied. The second-order rate constants for the inactivation of alpha-, beta T-, gamma T- and epsilon-thrombin by GdN were 1.41, 0.63, 0.33 and 1.91 microM-1.s-1 respectively. The kinetic properties of gdN were also investigated in the presence of different types of heparin, fractionated according to antithrombin III-binding affinity. Association rate constants of both gdN and antithrombin III with alpha-thrombin were obtained using unfractionated, low- and high-affinity heparin types. The different heparin types gave optimal rates of inhibition at similar heparin concentrations for both inhibitors. At optimal heparin concentrations, the rate of inactivation of alpha-thrombin by GdN was 0.5-1.2 nM-1.s-1, which suggests that, under these conditions, the interaction is diffusion-controlled.

scholarly journals Association Rate Constants as Determinants of Ligand Selectivity:Lessons from The Dopamine And The Serotonin Transporter

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
Michael Freissmuth ◽  
Peter Hasenhuetl ◽  
Sonja Sucic ◽  
Harald Sitte ◽  
Walter Sandtner
Keyword(s):  
Serotonin Transporter ◽  
Rate Constants ◽  
Association Rate ◽  
Association Rate Constants

On the chaperon mechanism for association rate constants: the formation of HO2 and O3

1996 ◽  
Vol 249 (3-4) ◽  
pp. 264-271 ◽  
Author(s):  
A.J.C. Varandas ◽  
A.A.C.C. Pais ◽  
J.M.C. Marques ◽  
W. Wang
Keyword(s):  
Rate Constants ◽  
Association Rate ◽  
Association Rate Constants
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