An x-ray and NMR study of the cubic phase at low water contents in the dodecyltrimethylammonium chloride/water system

1989 ◽  
Vol 93 (21) ◽  
pp. 7474-7478 ◽  
Author(s):  
O. Soederman ◽  
U. Olsson ◽  
T. C. Wong
Keyword(s):  
Water System ◽  
Cubic Phase ◽  
X Ray ◽  
Nmr Study ◽  
Dodecyltrimethylammonium Chloride ◽  
Chloride Water ◽  
Water Contents

scholarly journals Isoprenoid-chained lipid EROCOC17+4: a new matrix for membrane protein crystallization and a crystal delivery medium in serial femtosecond crystallography

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Kentaro Ihara ◽  
Masakatsu Hato ◽  
Takanori Nakane ◽  
Keitaro Yamashita ◽  
Tomomi Kimura-Someya ◽  
...  
Keyword(s):  
Protein Crystallization ◽  
Water System ◽  
Cubic Phase ◽  
Marked Contrast ◽  
X Ray ◽  
X Ray Scattering ◽  
Membrane Protein Crystallization ◽  
Almost All ◽  
Serial Femtosecond Crystallography ◽  
Delivery Medium

Abstract In meso crystallization of membrane proteins relies on the use of lipids capable of forming a lipidic cubic phase (LCP). However, almost all previous crystallization trials have used monoacylglycerols, with 1-(cis-9-octadecanoyl)-rac-glycerol (MO) being the most widely used lipid. We now report that EROCOC17+4 mixed with 10% (w/w) cholesterol (Fig. 1) serves as a new matrix for crystallization and a crystal delivery medium in the serial femtosecond crystallography of Adenosine A2A receptor (A2AR). The structures of EROCOC17+4-matrix grown A2AR crystals were determined at 2.0 Å resolution by serial synchrotron rotation crystallography at a cryogenic temperature, and at 1.8 Å by LCP-serial femtosecond crystallography, using an X-ray free-electron laser at 4 and 20 °C sample temperatures, and are comparable to the structure of the MO-matrix grown A2AR crystal (PDB ID: 4EIY). Moreover, X-ray scattering measurements indicated that the EROCOC17+4/water system did not form the crystalline LC phase at least down to − 20 °C, in marked contrast to the equilibrium MO/water system, which transforms into the crystalline LC phase below about 17 °C. As the LC phase formation within the LCP-matrix causes difficulties in protein crystallography experiments in meso, this feature of EROCOC17+4 will expand the utility of the in meso method.

An X-ray and NMR investigation of the lamellar liquid crystalline phase in the octylamine/octylammonium chloride/water system

1987 ◽  
Vol 118 (1) ◽  
pp. 233-242 ◽  
Author(s):  
Torbjo¨rn Wa¨rnheim ◽  
Bjo¨rn Bergensta˚hl ◽  
Ulf Henriksson ◽  
Ann-Charlotte Malmvik ◽  
Peder Nilsson
Keyword(s):  
Crystalline Phase ◽  
Liquid Crystalline ◽  
Water System ◽  
Liquid Crystalline Phase ◽  
X Ray ◽  
Chloride Water

Intermediate phases in the dodecyltrimethylammonium chloride/water system

1992 ◽  
Vol 12 (1) ◽  
pp. 71-81 ◽  
Author(s):  
C. Kang ◽  
O. Söderman ◽  
P. O. Eriksson ◽  
J. Stael Von Holstein
Keyword(s):  
Water System ◽  
Intermediate Phases ◽  
Dodecyltrimethylammonium Chloride ◽  
Chloride Water

NMR study of the phenol-formaldehyde-water system

1976 ◽  
Vol 41 (11) ◽  
pp. 3289-3293 ◽  
Author(s):  
Z. Fiala ◽  
M. Navrátil
Keyword(s):  
Phenol Formaldehyde ◽  
Water System ◽  
Nmr Study

Crystal and solution structure of (E)-1,2-bis(ethylsulphonyl)cyclobutane-1,2-dicarbonitrile

1989 ◽  
Vol 54 (12) ◽  
pp. 3253-3259
Author(s):  
Jaroslav Podlaha ◽  
Miloš Buděšínský ◽  
Jana Podlahová ◽  
Jindřich Hašek
Keyword(s):  
Hydrogen Peroxide ◽  
Solution Structure ◽  
Peroxide Oxidation ◽  
X Ray ◽  
X Ray Crystallography ◽  
Nmr Study ◽  
Hydrogen Peroxide Oxidation ◽  
Unusual Product ◽  
C Nmr

The unusual product of the reaction of 2-chloroacrylonitrile with ethane thiol and following hydrogen peroxide oxidation was found to be (E)-1,2-bis(ethylsulphonyl)cyclobutane-1,2-dicarbonitrile by means of X-ray crystallography. 1H and 13C NMR study of this compound has proven the same conformation of the molecule in solution.

scholarly journals Modeling of the Resonant X-ray Response of a Chiral Cubic Phase

Crystals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 214
Author(s):  
Timon Grabovac ◽  
Ewa Gorecka ◽  
Damian Pociecha ◽  
Nataša Vaupotič
Keyword(s):  
Absorption Edge ◽  
Unit Cell ◽  
Resonant Peak ◽  
Cubic Phase ◽  
Tensor Form ◽  
Resonant Enhancement ◽  
X Ray ◽  
X Ray Scattering ◽  
Thermotropic Liquid Crystals ◽  
Carbon Absorption

The structure of a continuous-grid chiral cubic phase made of achiral constituent molecules is a hot topic in the field of thermotropic liquid crystals. Several structural models have been proposed so far. Resonant X-ray scattering (RXS), which gives information on the molecular orientation in the unit cell, could be applied to select the most appropriate model. We modeled the RXS response for the recently proposed chiral cubic phase structure with an all-hexagon chiral continuous grid. A tensor form factor of a unit cell is constructed, which enables calculation of intensities of peaks for all Miller indices. We find that all the symmetry allowed peaks are resonantly enhanced, and their intensity is much stronger than the intensity of the symmetry forbidden (resonant) peaks. In particular, we predict that a strong resonant enhancement of the symmetry allowed peaks (011) and (002), not observed in a nonresonant scattering, could be observed by RXS at the carbon absorption edge. By RXS at the sulfur absorption edge, one might observe a resonant peak (113) and resonantly enhanced peak (233), and resonant enhancement of all the peaks that are observed in a nonresonant scattering, which probably hide the rest of the predicted resonant peaks.

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