Absorption spectroscopy and dynamic and static light-scattering studies of ethidium bromide binding to calf thymus DNA: implications for outside-binding and intercalation

1992 ◽  
Vol 96 (14) ◽  
pp. 6045-6055 ◽  
Author(s):  
Eckhard Nordmeier
Keyword(s):  
Light Scattering ◽  
Ethidium Bromide ◽  
Absorption Spectroscopy ◽  
Static Light Scattering ◽  
Calf Thymus Dna ◽  
Calf Thymus

Molecular Transformations in DNA under the Influence of UV-radiation

2020 ◽  
Vol 04 ◽  
Author(s):  
Vigen G. Barkhudaryan ◽  
Gayane V. Ananyan ◽  
Nelli H. Karapetyan
Keyword(s):  
Uv Radiation ◽  
Uv Irradiation ◽  
Absorption Spectroscopy ◽  
Calf Thymus Dna ◽  
Dilute Solutions ◽  
Concentrated Solutions ◽  
Buffer Solution ◽  
Low Concentration ◽  
Calf Thymus ◽  
Dna Solutions

Background: The processes of destruction and crosslinking of macromolecules occur simultaneously under the influence of ultraviolet (UV) radiation in synthetic polymers, dry DNA and their concentrated solutions. Objective: The effect of UV radiation on calf thymus DNA in dilute solutions subjected to UV- irradiation was studied in this work. Method: The calf thymus DNA was studied in dilute solutions using viscometry, absorption spectroscopy and electrophoresis. Results: It was shown, that at a low concentration of DNA in the buffer solution ([DNA] = 85 μg / ml) under the influence of UV radiation, the processes of destruction of macromolecules and an increase in their flexibility predominate, which is accompanied by a gradual decrease in the viscosity of their solution. In addition, due to the low concentration of the solution, intramolecular crosslinking of macromolecules predominates, which also reduces their size and, consequently, the viscosity of the solution. Conclusion: It was concluded, that in dilute DNA solutions, due to the predominance of the processes of intramolecular crosslinking of macromolecules over intermolecular, only constant processes of decreasing the sizes of DNA macromolecules occur. As a result, its solubility remains virtually unchanged during UV irradiation. The described comments are also excellently confirmed by the results of absorption spectroscopy and electrophoresis

Ethanol-Induced Condensation of Calf Thymus DNA Studied by Laser Light Scattering

1999 ◽  
Vol 103 (24) ◽  
pp. 5117-5121 ◽  
Author(s):  
K. B. Roy ◽  
T. Antony ◽  
A. Saxena ◽  
H. B. Bohidar
Keyword(s):  
Light Scattering ◽  
Laser Light ◽  
Laser Light Scattering ◽  
Calf Thymus Dna ◽  
Calf Thymus

Quasi-elastic light scattering by calf thymus DNA and ?DNA

Biopolymers ◽  
1975 ◽  
Vol 14 (3) ◽  
pp. 521-542 ◽  
Author(s):  
K. S. Schmitz ◽  
R. Pecora
Keyword(s):  
Light Scattering ◽  
Calf Thymus Dna ◽  
Elastic Light Scattering ◽  
Calf Thymus
Sign in / Sign up

Export Citation Format

Share Document