Fluorescence lifetimes of diphenylhexatriene in flat and bent bilayer lipid membranes

1991 ◽  
Vol 95 (10) ◽  
pp. 3892-3894 ◽  
Author(s):  
David Yogev ◽  
Angelio T. Todorov ◽  
Janos H. Fendler
Keyword(s):  
Lipid Membranes ◽  
Bilayer Lipid Membranes ◽  
Fluorescence Lifetimes ◽  
Bilayer Lipid

Intrinsic gramicidin fluorescence lifetimes in bilayer lipid membranes and in vesicles

1990 ◽  
Vol 68 (6) ◽  
pp. 888-896 ◽  
Author(s):  
Jose-Miguel Camaleńo-Delgado ◽  
Xiao Kang Zhao ◽  
Janos H. Fendler
Keyword(s):  
Lipid Membranes ◽  
Gramicidin A ◽  
Time Dependent ◽  
Bilayer Lipid Membranes ◽  
Fluorescence Lifetimes ◽  
Iodide Ions ◽  
Bilayer Lipid ◽  
Solid States ◽  
Liquid States ◽  
Average Fluorescence

Intrinsic Gramicidin A′ tryptophan steady-state fluorescence anisotropies and fluorescence lifetimes have been determined in bilayer lipid membranes (BLMs) prepared from glyceryl monooleate (GMO). In GMO BLMs, fluorescence anisotropy, the r value, was found to be 0.05 ± 0.02. Decays of Gramicidin A′ fluorescence intensities were fitted to the sum of three exponentials (τ1, τ2, and τ3) and appropriate pre-exponentials (A1, A2, and A3). These values allowed for the assessment of average fluorescence lifetimes, [Formula: see text]. These values related to those determined in vesicles prepared from dipalmitoylphosphatidylcholine (DPPC), dioleoylphosphatidylcholine (DOPC), distearoylphosphatidylcholine (DSPC), and diphytanoylphosphatidylcholine (DPhPC). In BLMs, [Formula: see text], 3.6 ns, and 2.3 ns were determined for vertically, horizontally, and unpolarized average fluorescence lifetimes, respectively. Increasing the applied potential across the BLM from 0 to 80 mV increased [Formula: see text] from 2.2 ns to 4.9 ns and τ1 from 0.43 ± 0.05 to 0.73 ± 0.06 ns, as well as the contributions and lifetimes of the longer lived fluorescence (A2 and A3, τ2 and τ3). The emission maximum of Gramicidin A′ (334 nm in DPPC) and the absence of quenching by iodide ions indicated complete incorporation of the polypeptide into vesicles. The r values were of the order of 0.10 in vesicles prepared from DPPC and DSPC, both in the absence and in the presence of added 1.2 × 10−4 M CsCl. In vesicles prepared from DOPC and DPhPC, r values increased to 0.13 and 0.14 in water and to 0.15 and 0.20 in 1.2 × 10−4 M CsCl, respectively. At 25.0 °C, the temperature of the measurements, DPPC and DSPC are in their "solid" states, but DOPC and DPhPC are in their "liquid" states, [Formula: see text] values for Gramicidin A′ in vesicles prepared from DPPC, DOPC, and DSPC were all in the 3.0 ± 0.3 ns range. In DPhPC vesicles, [Formula: see text] was determined. Time-dependent anisotropics became observable in DOPC and DPhPC vesicles, particularly in the presence of 1.2 × 10−4 M CsCl. Keywords: gramicidin, fluorescence lifetimes, vesicles, bilayer lipid membranes, time-dependent anisotropics.

Stabilization of bilayer lipid membranes on solid supports by trehalose

1996 ◽  
Vol 39 (2) ◽  
pp. 299-302 ◽  
Author(s):  
T. Hianik ◽  
J. Dlugopolsky ◽  
M. Gyeppessova ◽  
B. Sivak ◽  
H.T. Tien ◽  
...  
Keyword(s):  
Lipid Membranes ◽  
Bilayer Lipid Membranes ◽  
Solid Supports ◽  
Bilayer Lipid

Impact of membrane protein-lipid interactions on formation of bilayer lipid membranes on SAM-modified gold electrode

2021 ◽  
Vol 373 ◽  
pp. 137888
Author(s):  
Masaru Kato ◽  
Yuya Masuda ◽  
Narumi Yoshida ◽  
Takehiko Tosha ◽  
Yoshitsugu Shiro ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Lipid Membranes ◽  
Gold Electrode ◽  
Bilayer Lipid Membranes ◽  
Lipid Interactions ◽  
Bilayer Lipid

scholarly journals The Use of Tethered Bilayer Lipid Membranes to Identify the Mechanisms of Antimicrobial Peptide Interactions with Lipid Bilayers

Antibiotics ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 12 ◽  
Author(s):  
Amani Alghalayini ◽  
Alvaro Garcia ◽  
Thomas Berry ◽  
Charles Cranfield
Keyword(s):  
New Zealand ◽  
Patch Clamp ◽  
Lipid Bilayers ◽  
Lipid Membranes ◽  
Antimicrobial Agents ◽  
Bilayer Lipid Membranes ◽  
Bilayer Lipid ◽  
Black Lipid Membranes ◽  
Peptide Interactions ◽  
New Research

This review identifies the ways in which tethered bilayer lipid membranes (tBLMs) can be used for the identification of the actions of antimicrobials against lipid bilayers. Much of the new research in this area has originated, or included researchers from, the southern hemisphere, Australia and New Zealand in particular. More and more, tBLMs are replacing liposome release assays, black lipid membranes and patch-clamp electrophysiological techniques because they use fewer reagents, are able to obtain results far more quickly and can provide a uniformity of responses with fewer artefacts. In this work, we describe how tBLM technology can and has been used to identify the actions of numerous antimicrobial agents.

First Observation of the Converse Flexoelectric Effect in Bilayer Lipid Membranes

1994 ◽  
Vol 98 (12) ◽  
pp. 3076-3079 ◽  
Author(s):  
A. T. Todorov ◽  
A. G. Petrov ◽  
J. H. Fendler
Keyword(s):  
Lipid Membranes ◽  
Bilayer Lipid Membranes ◽  
Bilayer Lipid ◽  
Flexoelectric Effect

Membrane supported bilayer lipid membranes array: preparation, stability and ion-channel insertion

2002 ◽  
Vol 460 (1) ◽  
pp. 23-34 ◽  
Author(s):  
G Favero ◽  
A D’Annibale ◽  
L Campanella ◽  
R Santucci ◽  
T Ferri
Keyword(s):  
Ion Channel ◽  
Lipid Membranes ◽  
Bilayer Lipid Membranes ◽  
Bilayer Lipid ◽  
Supported Bilayer
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