Interfacial Tension of Hydrocarbon + Different pH Aqueous phase Systems in the Presence of Triton X-100

2010 ◽  
Vol 49 (7) ◽  
pp. 3228-3235 ◽  
Author(s):  
Javad Saien ◽  
Somayeh Akbari
Keyword(s):  
Interfacial Tension ◽  
Aqueous Phase ◽  
Phase Systems ◽  
Triton X

Interfacial Tension of Aqueous Three‐Phase Systems Formed by Triton X‐100/PEG/Dextran

2006 ◽  
Vol 27 (7) ◽  
pp. 927-930 ◽  
Author(s):  
Ke Ruan ◽  
Bao‐Huai Wang ◽  
Jin‐Xin Xiao ◽  
Jiao‐Ning Tang
Keyword(s):  
Interfacial Tension ◽  
Three Phase ◽  
Phase Systems ◽  
Triton X

scholarly journals Glycosyl-phosphatidylinositol-anchored membrane proteins can be distinguished from transmembrane polypeptide-anchored proteins by differential solubilization and temperature-induced phase separation in Triton X-114

1991 ◽  
Vol 280 (3) ◽  
pp. 745-751 ◽  
Author(s):  
N M Hooper ◽  
A Bashir
Keyword(s):  
Phase Separation ◽  
Membrane Proteins ◽  
Aqueous Phase ◽  
Hydrophobic Membrane ◽  
Rich Phase ◽  
Glycosyl Phosphatidylinositol ◽  
Ionic Detergent ◽  
Membrane Spanning ◽  
Triton X ◽  
Insoluble Pellet

Treatment of kidney microvillar membranes with the non-ionic detergent Triton X-114 at 0 degrees C, followed by low-speed centrifugation, generated a detergent-insoluble pellet and a detergent-soluble supernatant. The supernatant was further fractionated by phase separation at 30 degrees C into a detergent-rich phase and a detergent-depleted or aqueous phase. Those ectoenzymes with a covalently attached glycosyl-phosphatidylinositol (G-PI) membrane anchor were recovered predominantly (greater than 73%) in the detergent-insoluble pellet. In contrast, those ectoenzymes anchored by a single membrane-spanning polypeptide were recovered predominantly (greater than 62%) in the detergent-rich phase. Removal of the hydrophobic membrane-anchoring domain from either class of ectoenzyme resulted in the proteins being recovered predominantly (greater than 70%) in the aqueous phase. This technique was also applied to other membrane types, including pig and human erythrocyte ghosts, where, in both cases, the G-PI-anchored acetylcholinesterase partitioned predominantly (greater than 69%) into the detergent-insoluble pellet. When the microvillar membranes were subjected only to differential solubilization with Triton X-114 at 0 degrees C, the G-PI-anchored ectoenzymes were recovered predominantly (greater than 63%) in the detergent-insoluble pellet, whereas the transmembrane-polypeptide-anchored ectoenzymes were recovered predominantly (greater than 95%) in the detergent-solubilized supernatant. Thus differential solubilization and temperature-induced phase separation in Triton X-114 distinguished between G-PI-anchored membrane proteins, transmembrane-polypeptide-anchored proteins and soluble, hydrophilic proteins. This technique may be more useful and reliable than susceptibility to release by phospholipases as a means of identifying a G-PI anchor on an unpurified membrane protein.

Single stage and countercurrent extraction of 5-hydroxymethylfurfural from aqueous phase systems

2016 ◽  
Vol 283 ◽  
pp. 251-259 ◽  
Author(s):  
Eva C. Sindermann ◽  
Alexander Holbach ◽  
André de Haan ◽  
Norbert Kockmann
Keyword(s):  
Aqueous Phase ◽  
Single Stage ◽  
Countercurrent Extraction ◽  
Phase Systems

scholarly journals The hyaluronate synthase from a eukaryotic cell line

1993 ◽  
Vol 290 (3) ◽  
pp. 791-795 ◽  
Author(s):  
L Klewes ◽  
E A Turley ◽  
P Prehm
Keyword(s):  
Monoclonal Antibodies ◽  
Phase Separation ◽  
Affinity Chromatography ◽  
Cell Line ◽  
Aqueous Phase ◽  
Plasma Membranes ◽  
Eukaryotic Cell ◽  
Molecular Masses ◽  
Triton X ◽  
Hyaluronate Synthase

The hyaluronate synthase complex was identified in plasma membranes from B6 cells. It contained two subunits of molecular masses 52 kDa and 60 kDa which bound the precursor UDP-GlcA in digitonin solution and partitioned into the aqueous phase, together with nascent hyaluronate upon Triton X-114 phase separation. The 52 kDa protein cross-reacted with poly- and monoclonal antibodies raised against the streptococcal hyaluronate synthase and the 60 kDa protein was recognized by monoclonal antibodies raised against a hyaluronate receptor. The 52 kDa protein was purified to homogeneity by affinity chromatography with monoclonal anti-hyaluronate synthase.

Non-aqueous phase liquids distribution in three-fluid phase systems in double-porosity soil media: Experimental investigation using image analysis

2018 ◽  
Vol 7 ◽  
pp. 133-142 ◽  
Author(s):  
Motasem Y.D. Alazaiza ◽  
Su Kong Ngien ◽  
Mustafa M. Bob ◽  
Samira A. Kamaruddin ◽  
Wan Mohd Faizal Ishak
Keyword(s):  
Experimental Investigation ◽  
Image Analysis ◽  
Aqueous Phase ◽  
Fluid Phase ◽  
Double Porosity ◽  
Soil Media ◽  
Phase Systems

Interfacial tension behavior between decane-water with triton X-100 under various microwave irradiation conditions

2019 ◽  
Vol 56 (4) ◽  
pp. 1343-1351 ◽  
Author(s):  
Satoshi Sonobe ◽  
Yosuke Shibata ◽  
Naoya Minamishima ◽  
Yusuke Asakuma ◽  
Anita Hyde ◽  
...  
Keyword(s):  
Microwave Irradiation ◽  
Interfacial Tension ◽  
Triton X

Erythrocyte partitioning in dextran—poly(ethylene glycol) aqueous phase systems

1992 ◽  
Vol 609 (1-2) ◽  
pp. 219-227 ◽  
Author(s):  
Harry Walter ◽  
Frank D. Raymond ◽  
Derek Fisher
Keyword(s):  
Ethylene Glycol ◽  
Aqueous Phase ◽  
Poly Ethylene Glycol ◽  
Phase Systems ◽  
Poly Ethylene
Sign in / Sign up

Export Citation Format

Share Document