Combined Application of in Situ FBRM, ATR-FTIR, and Raman on Polymorphism Transformation Monitoring During the Cooling Crystallization

2012 ◽  
pp. 120912161452001 ◽  
Author(s):  
Yingying Zhao ◽  
Junsheng Yuan ◽  
Zhiyong Ji ◽  
Jingkang Wang ◽  
Sohrab Rohani
Keyword(s):  
Combined Application ◽  
Cooling Crystallization

Process Control of Seeded Batch Cooling Crystallization of the Metastable α-Form Glycine Using an In-Situ ATR-FTIR Spectrometer and an In-Situ FBRM Particle Counter

2004 ◽  
Vol 4 (5) ◽  
pp. 949-953 ◽  
Author(s):  
Norihito Doki ◽  
Hiroya Seki ◽  
Kiyoteru Takano ◽  
Haruki Asatani ◽  
Masaaki Yokota ◽  
...  
Keyword(s):  
Process Control ◽  
Particle Counter ◽  
Cooling Crystallization ◽  
Ftir Spectrometer ◽  
Batch Cooling

Seeding in Situ the Cooling Crystallization of Adipic Acid using Ultrasound

2012 ◽  
Vol 12 (4) ◽  
pp. 1727-1735 ◽  
Author(s):  
O. Narducci ◽  
A. G. Jones
Keyword(s):  
Adipic Acid ◽  
Cooling Crystallization

Novel Nitrospira-like bacteria as dominant nitrite-oxidizers in biofilms from wastewater treatment plants: diversity and in situ physiology

2000 ◽  
Vol 41 (4-5) ◽  
pp. 85-90 ◽  
Author(s):  
H. Daims ◽  
P.H. Nielsen ◽  
J.L. Nielsen ◽  
S. Juretschko ◽  
M. Wagner
Keyword(s):  
Wastewater Treatment ◽  
In Situ Hybridization ◽  
16S Rrna ◽  
Fluorescent In Situ Hybridization ◽  
Wastewater Treatment Plants ◽  
Combined Application ◽  
Fundamental Understanding ◽  
Unculturable Bacteria ◽  
Rrna Sequences

The frequency and distribution of putatively nitrite-oxidizing, Nitrospira- like bacteria in nitrifying biofilms from two reactors receiving wastewater with different ammonia and salt concentrations were observed by fluorescent in situ hybridization. For this purpose, new 16S rRNA-directed oligonucleotide probes targeting the bacterial phylum Nitrospira and the three main lineages within this phylum were developed and evaluated. The diversity of Nitrospira-like bacteria in the reactors was additionally investigated by retrieval and comparative analysis of full 16S rRNA sequences from the biofilms. We found that, despite of the differences in the influent composition, Nitrospira-like bacteria form dominant populations in both reactors. In addition, first insights into the physiology of these still unculturable bacteria were obtained by the incubation of active biofilm samples with radioactively labeled substrates followed by the combined application of fluorescent in situ hybridization and microautoradiography. The results are discussed in consideration of the frequently observed dominance of Nitrospira-like bacteria in nitrifying bioreactors. Consequently, high priority should be assigned to future studies on the ecology and physiology of these organisms in order to increase our fundamental understanding of nitrogen cycling and to enable knowledge-driven future improvements of nitrifying wastewater treatment plants.

Investigation into the Cooling Crystallization and Transformations of Carbamazepine Using in Situ FBRM and PVM

2013 ◽  
Vol 17 (11) ◽  
pp. 1406-1412 ◽  
Author(s):  
Wenju Liu ◽  
Hongyuan Wei ◽  
Junting Zhao ◽  
Simon Black ◽  
Chen Sun
Keyword(s):  
Cooling Crystallization

Contribution of Ca2+-Permeable AMPA/KA Receptors to Glutamate-Induced Ca2+ Rise in Embryonic Lumbar Motoneurons In Situ

2000 ◽  
Vol 83 (1) ◽  
pp. 50-59 ◽  
Author(s):  
Friedrich Metzger ◽  
Anna Kulik ◽  
Michael Sendtner ◽  
Klaus Ballanyi
Keyword(s):  
Ampa Receptors ◽  
Cyclopiazonic Acid ◽  
Channel Blockers ◽  
Voltage Gated ◽  
Fura 2 ◽  
Combined Application ◽  
Predominant Component ◽  
Intracellular Ca ◽  
Trophic Signaling

Intracellular Ca2+([Ca2+]i) was fluorometrically measured with fura-2 in lumbar motoneurons of acutely isolated spinal cord slices from embryonic rats. In ester-loaded cells, bath-applied glutamate (3 μM to 1 mM) evoked a [Ca2+]i increase by up to 250 nM that was abolished by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) plus 2-amino-5-phosphonovalerate (APV). CNQX or APV alone reduced the response by 82 and 25%, respectively. The glutamatergic agonists kainate (KA), quisqualate (QUI), and S-α-amino-3-hydroxy-5-methyl-4-isoxalone (S-AMPA) evoked a similar [Ca2+]i transient as glutamate. N-methyl-d-aspartate (NMDA) was only effective to increase [Ca2+]i in Mg2+-free saline, whereas [1S,3R]-1-aminocyclopentane-1,3-dicarboxylic acid ([1S,3R]-ACPD) had no effect. The glutamate-induced [Ca2+]i rise was suppressed in Ca2+-free superfusate. Depletion of Ca2+ stores with cyclopiazonic acid (CPA) did not affect the response. Thirty-six percent of the [Ca2+]i increase in response to membrane depolarization induced by a 50 mM K+ solution persisted on combined application of the voltage-gated Ca2+channel blockers nifedipine, ω-conotoxin-GVIA and ω-agatoxin-IVA. In fura-2 dialyzed motoneurons, the glutamate-induced [Ca2+]i increase was attenuated by ∼70% after changing from current to voltage clamp. Forty percent of the remaining [Ca2+]i transient and 20% of the concomitant inward current of 0.3 nA were blocked by Joro spider toxin-3 (JSTX). The results show that voltage-gated Ca2+channels, including a major portion of R-type channels, constitute the predominant component of glutamate-induced [Ca2+]i rises. NMDA and Ca2+-permeable KA/AMPA receptors contribute about equally to the remaining component of the Ca2+ rise. The results substantiate previous assumptions that Ca2+ influx through JSTX-sensitive KA/AMPA receptors is involved in (trophic) signaling in developing motoneurons.

scholarly journals Molekuláris citogenetikai vizsgálatok Baranya és Tolna megye plazmasejtes myelomában szenvedő betegein

2019 ◽  
Vol 160 (24) ◽  
pp. 944-951
Author(s):  
Szabolcs Kosztolányi ◽  
Bálint Horváth ◽  
Diána Hosnyánszki ◽  
László Kereskai ◽  
Erzsébet Sziládi ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Plasma Cell ◽  
Chain Gene ◽  
Plasma Cell Myeloma ◽  
Genetic Aberrations ◽  
Molecular Cytogenetic ◽  
Combined Application ◽  
Dependent Probe

Abstract: Introduction: Plasma cell myeloma is a hematological malignancy with heterogeneous genomic landscape and diverse clinical course. Recurrent chromosomal and subchromosomal aberrations commonly occur in this entity and are associated with the pathogenesis and progression of the disease. The identification of these alterations aids genetic characterization, classification and prognostication of patients. Aim: Molecular cytogenetic investigations of plasma cell myeloma patients treated at the University of Pécs Clinical Center and János Balassa County Hospital of Tolna County, Szekszárd, between 2005 and 2018 were evaluated in our study. Method: 231 patients were screened for genetic aberrations using fluorescence in situ hybridization. Translocations involving the immunoglobulin heavy chain gene, losses of 1p and 17p chromosome arms, gains of 1q chromosome arm and unbalanced aberrations of chromosome 13 were investigated. Losses and gains of 1p, 1q, 5q, 12p, 13q, 16q and 17p chromosome arms were analyzed using multiplex ligation-dependent probe amplification in 42 patients. During the investigated period, 116 bone marrow karyotyping was also performed. Results: In total, 233 genetic aberrations were identified using our targeted approaches; the frequency of specific aberrations correlated with data of the recent literature. Concordance of results gained by fluorescence in situ hybridization and multiplex ligation-dependent probe amplification was 96.2% by analyzing the same chromosome arms. The latter technique revealed 21 additional genetic aberrations in 16/42 patient samples (38%) as compared to fluorescence in situ hybridization. Conclusions: Our results suggest that the combined application of the two molecular cytogenetic methods may facilitate a more detailed characterization of genetic aberrations of plasma cell myeloma patients in Hungary. Orv Hetil. 2019; 160(24): 944–951.

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