A Time-Resolved Fluorescence Diphenylhexatriene (DPH) Anisotropy Characterization of a Series of Model Lipid Constructs for the Sperm Plasma Membrane

2006 ◽  
Vol 45 (21) ◽  
pp. 6939-6945 ◽  
Author(s):  
Gregory M. Troup ◽  
Steven P. Wrenn ◽  
Meirav Apel-Paz ◽  
Gustavo F. Doncel ◽  
T. Kyle Vanderlick
Keyword(s):  
Plasma Membrane ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Sperm Plasma Membrane

Characterization of human sperm plasma membrane: Glycolipids and polypeptides

1987 ◽  
Vol 243 (2) ◽  
pp. 339-346 ◽  
Author(s):  
S. R. Mack ◽  
L. J. D. Zaneveld ◽  
R. N. Peterson ◽  
W. Hunt ◽  
L. D. Russell
Keyword(s):  
Plasma Membrane ◽  
Human Sperm ◽  
Sperm Plasma Membrane

scholarly journals Synthesis and Characterization of Time-resolved Fluorescence Probes for Evaluation of Competitive Binding to Melanocortin Receptors

2013 ◽  
Vol 21 (17) ◽  
pp. 5029-5038 ◽  
Author(s):  
Ramesh Alleti ◽  
Josef Vagner ◽  
Dilani Chathurika Dehigaspitiya ◽  
Valerie E. Moberg ◽  
N.G.R.D. Elshan ◽  
...  
Keyword(s):  
Competitive Binding ◽  
Fluorescence Probes ◽  
Synthesis And Characterization ◽  
Melanocortin Receptors ◽  
Time Resolved Fluorescence ◽  
Time Resolved

Characterization of Plasma-Modified Fluoropolymer Surfaces Using Steady-State and Time-Resolved Fluorescence Spectroscopy

1994 ◽  
Vol 48 (5) ◽  
pp. 630-637 ◽  
Author(s):  
Ming Li ◽  
Michaeleen L. Pacholski ◽  
Frank V. Bright
Keyword(s):  
Steady State ◽  
Fluorescence Spectroscopy ◽  
Sulfonyl Chloride ◽  
Sodium Dodecylsulfate ◽  
Covalent Immobilization ◽  
Ambient Conditions ◽  
Time Resolved Fluorescence ◽  
Decay Kinetics ◽  
Time Resolved

Poly(hexafluoropropylene-co-tetrafluoroethylene) (FEP) has been widely used in biotechnology because of its unique surface properties and biocompatibility. Recent work from our group has shown that plasma discharge-modified FEP can be used as the substratum for development of a very stable immunosensor. This result has prompted us to study further this new surface under ambient conditions. In this paper, we report on the covalent immobilization of a pyrene residue (-Py) onto FEP-APS (FEP-aminopropyl silane) surfaces and the characterization of FEP-APS-Py using steady-state and time-resolved fluorescence spectroscopy. Among the immobilization schemes tested, we found that the covalent coupling of pyrene-sulfonyl chloride to FEP-APS is the easiest and yields the most photostable FEP-APS-Py derivative. Steady-state emission spectra of FEP-APS-Py in contact with H2O, β-cyclodextrin (β-CD), and sodium dodecylsulfate (SDS) aqueous solutions differ considerably from those of Py-SO3 in solution. Time-resolved fluorescence spectroscopy of FEP-APS-Py demonstrates that the decay kinetics are strongly affected by the presence of ionic quenchers and molecular oxygen, as well as β-CD and SDS. The results are consistent with the suggestion that the APS-Py moiety undergoes a slow time-dependent reconfiguration at the FEP/APS interface.

scholarly journals Characterization of F2N12S in Cell Membranes using Time-Resolved Fluorescence Techniques

2018 ◽  
Vol 114 (3) ◽  
pp. 98a-99a
Author(s):  
Donald S. Anderson ◽  
Matthew J. Sydor ◽  
Harmen B. Steele ◽  
J.B.A. Ross ◽  
Holian Andrij
Keyword(s):  
Cell Membranes ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Fluorescence Techniques
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