Preparation and Application of Novel EOPO−IDA−Metal Polymer as Recyclable Metal Affinity Ligand in Aqueous Two-Phase Systems

2006 ◽  
Vol 45 (5) ◽  
pp. 1774-1779 ◽  
Author(s):  
Jin Lu ◽  
Dong-Qiang Lin ◽  
Shan-Jing Yao
Keyword(s):  
Two Phase ◽  
Metal Affinity ◽  
Affinity Ligand ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Metal Polymer

Surface heterogeneity of bovine sperm revealed by aqueous two-phase partition

1991 ◽  
Vol 11 (5) ◽  
pp. 265-273 ◽  
Author(s):  
Elizabeth J. Cartwright ◽  
Allison Cowin ◽  
Paul T. Sharpe
Keyword(s):  
Surface Properties ◽  
Seminal Fluid ◽  
Surface Heterogeneity ◽  
Two Phase ◽  
Surface Molecules ◽  
Affinity Ligand ◽  
Bovine Sperm ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Two Phase Partition

Ejaculated, bovine sperm have been subjected to multiple partition in aqueous two-phase systems. This partition, carried out in a countercurrent fashion, reveals heterogeneity of the sperm population with respect to surface properties. The sperm, when partitioned in phase systems that detect non-change associated surface properties (change-insensitive) are largely distributed as two distinct populations. In charge-sensitive phase systems (which principally detect cell surface molecules carrying charge) the sperm do not show any obvious surface heterogeneity. Considerable heterogeneity is revealed in affinity-ligand phase systems containing palmitic acid coupled to one of the phase components-poly(ethylene glycol). There is a difference in surface heterogeneity between sperm which have been washed in buffer or left unwashed, direct from the ejaculate. This is indicative of weak adsorption of proteins to the sperm surface in seminal fluid. These results show that bovine ejaculated sperm is a heterogeneous cell population having unequal distributions of a number of different surface molecules.

Continuous purification of antibodies from cell culture supernatant with aqueous two-phase systems: From concept to process

2013 ◽  
Vol 8 (3) ◽  
pp. 352-362 ◽  
Author(s):  
Paula A. J. Rosa ◽  
Ana M. Azevedo ◽  
S. Sommerfeld ◽  
Martina Mutter ◽  
Werner Bäcker ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Supernatant ◽  
Cell Culture Supernatant ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Optimized and Scalable Green Extraction of Pristimerin, an Anticancerigen from Mortonia greggii, by Ethanol–Phosphate Aqueous Two-Phase Systems

2021 ◽  
Vol 60 (15) ◽  
pp. 5403-5410
Author(s):  
Martha Alicia González-Félix ◽  
Luis Alberto Mejía-Manzano ◽  
Bertha A. Barba-Dávila ◽  
Sergio O. Serna-Saldívar ◽  
José González-Valdez
Keyword(s):  
Two Phase ◽  
Green Extraction ◽  
Aqueous Two Phase Systems ◽  
Phase Systems
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