Sequential Parameter Estimation for Large-Scale Systems with Multiple Data Sets. 2. Application to an Industrial Coke-Oven-Gas Purification Process

2004 ◽  
Vol 43 (15) ◽  
pp. 4350-4362 ◽  
Author(s):  
Richard Faber ◽  
Pu Li ◽  
Günter Wozny
2020 ◽  
Vol 248 ◽  
pp. 119183 ◽  
Author(s):  
Jingying Li ◽  
Suisui Zhang ◽  
Yan Nie ◽  
Xiaoxun Ma ◽  
Long Xu ◽  
...  

mBio ◽  
2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Weibo Zheng ◽  
Chundi Wang ◽  
Michael Lynch ◽  
Shan Gao

ABSTRACT How to achieve protein diversity by genome and transcriptome processing is essential for organismal complexity and adaptation. The present work identifies that the macronuclear genome of Halteria grandinella, a cosmopolitan unicellular eukaryote, is composed almost entirely of gene-sized nanochromosomes with extremely short nongenic regions. This challenges our usual understanding of chromosomal structure and suggests the possibility of novel mechvanisms in transcriptional regulation. Comprehensive analysis of multiple data sets reveals that Halteria transcription dynamics are influenced by: (i) nonuniform nanochromosome copy numbers correlated with gene-expression level; (ii) dynamic alterations at both the DNA and RNA levels, including alternative internal eliminated sequence (IES) deletions during macronucleus formation and large-scale alternative splicing in transcript maturation; and (iii) extremely short 5′ and 3′ untranslated regions (UTRs) and universal TATA box-like motifs in the compact 5′ subtelomeric regions of most chromosomes. This study broadens the view of ciliate biology and the evolution of unicellular eukaryotes, and identifies Halteria as one of the most compact known eukaryotic genomes, indicating that complex cell structure does not require complex gene architecture.


2020 ◽  
Vol 76 (8) ◽  
pp. 771-777 ◽  
Author(s):  
Gustavo M. A. Lima ◽  
Vladimir O. Talibov ◽  
Elmir Jagudin ◽  
Céleste Sele ◽  
Maria Nyblom ◽  
...  

Advances in synchrotron storage rings and beamline automation have pushed data-collection rates to thousands of data sets per week. With this increase in throughput, massive projects such as in-crystal fragment screening have become accessible to a larger number of research groups. The quality of support offered at large-scale facilities allows medicinal chemistry-focused or biochemistry-focused groups to supplement their research with structural biology. Preparing the experiment, analysing multiple data sets and prospecting for interesting complexes of protein and fragments require, for both newcomers and experienced users, efficient management of the project and extensive computational power for data processing and structure refinement. Here, FragMAX, a new complete platform for fragment screening at the BioMAX beamline of the MAX IV Laboratory, is described. The ways in which users are assisted in X-ray-based fragment screenings and in which the fourth-generation storage ring available at the facility is best exploited are also described.


2021 ◽  
Vol 257 ◽  
pp. 117862
Author(s):  
Lucas de Oliveira Carneiro ◽  
Suênia Fernandes de Vasconcelos ◽  
Gilvan Wanderley de Farias Neto ◽  
Romildo Pereira Brito ◽  
Karoline Dantas Brito

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