Increased Resolution in High-Performance Liquid Chromatograph Spectra of High-Molecular-Weight Organic Components of Bayer Liquors

2003 ◽  
Vol 42 (26) ◽  
pp. 6673-6681 ◽  
Author(s):  
Thelma J. Whelan ◽  
G. S. Kamali Kannangara ◽  
Michael A. Wilson
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
High Performance ◽  
High Performance Liquid Chromatograph ◽  
Liquid Chromatograph ◽  
Organic Components ◽  
Bayer Liquors

Preparation and Characterization of the High Molecular Weight [3H]Hyaluronic Acid

1992 ◽  
Vol 57 (10) ◽  
pp. 2151-2156 ◽  
Author(s):  
Peter Chabreček ◽  
Ladislav Šoltés ◽  
Hynek Hradec ◽  
Jiří Filip ◽  
Eduard Orviský
Keyword(s):  
Molecular Weight ◽  
Hyaluronic Acid ◽  
High Molecular Weight ◽  
Methyl Bromide ◽  
High Performance ◽  
Hydrogen Atoms ◽  
Isolation And Characterization ◽  
Labelling Procedure ◽  
Enzymatic Depolymerization

Two methods for the preparation of high molecular weight [3H]hyaluronic acid were investigated. In the first one, hydrogen atoms in the molecule were replaced by tritium. This isotopic substitution was performed in aqueous solution using Pd/CaCO3 as the catalyst. In the second method, the high molecular weight hyaluronic acid was alkylated with [3H]methyl bromide in liquid ammonia at a temperature of -33.5 °C. High-performance gel permeation chromatographic separation method was used for the isolation and characterization of the high molecular weight [3H]hyaluronic acid. Molecular weight parameters for the labelled biopolymers were Mw = 128 kDa, Mw/Mn = 1.88 (first method) and Mw = 268 kDa, Mw/Mn = 1.55 (second method). The high molecular weight [3H]hyaluronic acid having Mw = 268 kDa was degraded further by specific hyaluronidase. Products of the enzymatic depolymerization were observed to be identical for both, labelled and cold biopolymer. This finding indicates that the described labelling procedure using [3H]methyl bromide does not induce any major structural rearrangements in the molecule.

Efficient high performance liquid chromatograph/ultraviolet method for determination of diclofenac and 4′-hydroxydiclofenac in rat serum

2006 ◽  
Vol 830 (2) ◽  
pp. 231-237 ◽  
Author(s):  
Lata Kaphalia ◽  
Bhupendra S. Kaphalia ◽  
Santosh Kumar ◽  
Mary F. Kanz ◽  
Mary Treinen-Moslen
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatograph ◽  
Rat Serum ◽  
Liquid Chromatograph

Semi-bio-based aromatic polyamides from 2,5-furandicarboxylic acid: toward high-performance polymers from renewable resources

RSC Advances ◽  
2016 ◽  
Vol 6 (90) ◽  
pp. 87013-87020 ◽  
Author(s):  
Kaiju Luo ◽  
Yan Wang ◽  
Junrong Yu ◽  
Jing Zhu ◽  
Zuming Hu
Keyword(s):  
Mechanical Properties ◽  
Thermal Stability ◽  
Molecular Weight ◽  
High Molecular Weight ◽  
Renewable Resources ◽  
High Performance ◽  
Good Thermal Stability ◽  
Aromatic Polyamides ◽  
High Performance Polymers ◽  
Furandicarboxylic Acid

Aromatic furanic polyamides with relatively high molecular weight were synthesized, and good thermal stability and mechanical properties were demonstrated.

Use of a flow-injection sample manipulator as an interface between a "high-performance" liquid chromatograph and an atomic absorption spectrophotometer.

1981 ◽  
Vol 27 (9) ◽  
pp. 1546-1550 ◽  
Author(s):  
B W Renoe ◽  
C E Shideler ◽  
J Savory
Keyword(s):  
Atomic Absorption ◽  
Flow Injection ◽  
Metal Binding ◽  
High Performance ◽  
Atomic Absorption Spectrophotometry ◽  
Clinical Samples ◽  
High Performance Liquid Chromatograph ◽  
Liquid Chromatograph ◽  
Flame Atomic Absorption ◽  
Column Eluate

Abstract We describe an integrated, molecular-absorbance, atomic absorption instrument for studying metal/ligand binding in clinical samples. For an interface between the "high-performance" liquid chromatograph and the atomic absorption instrument we used a flow-injection sample manipulator, thus allowing both the chromatograph and the atomic absorption detector to operate at their separate optimum conditions. After specimen separation with a gel permeation column, we measured the molecular components of the column eluate by molecular absorbance spectrometry and the atomic components (calcium and magnesium) by flame atomic absorption spectrophotometry. This instrument system is capable of separating and analyzing multiple components within 20 min of injection of the sample on the column. The chromatograms presented demonstrate the utility of the system for investigating metal binding to a variety of ligands in clinical samples.

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