In Vitro Sustained Release of Human Immunoglobulin G from Biodegradable Microspheres

2001 ◽  
Vol 40 (3) ◽  
pp. 933-948 ◽  
Author(s):  
Hsin Min Wong ◽  
Jian Jun Wang ◽  
Chi-Hwa Wang
Keyword(s):  
Sustained Release ◽  
Immunoglobulin G ◽  
Human Immunoglobulin ◽  
Biodegradable Microspheres ◽  
Human Immunoglobulin G

In vitro glycation of human immunoglobulin G

2004 ◽  
Vol 345 (1-2) ◽  
pp. 105-111 ◽  
Author(s):  
Anto Vrdoljak ◽  
Anda Trescec ◽  
Bojan Benko ◽  
Dragica Hecimovic ◽  
Mirjana Simic
Keyword(s):  
Immunoglobulin G ◽  
Human Immunoglobulin ◽  
Human Immunoglobulin G

Functional in vitro studies of recombinant human immunoglobulin G and immunoglobulin A anti-D

Transfusion ◽  
2007 ◽  
Vol 47 (2) ◽  
pp. 306-315 ◽  
Author(s):  
Leif Kofoed Nielsen ◽  
Trine Hefsgaard Green ◽  
Lars Norderhaug ◽  
Inger Sandlie ◽  
Morten Hanefeld Dziegiel
Keyword(s):  
Immunoglobulin G ◽  
Immunoglobulin A ◽  
In Vitro Studies ◽  
Human Immunoglobulin ◽  
Human Immunoglobulin G

scholarly journals Covalent Polymyxin B Conjugate with Human Immunoglobulin G as an Antiendotoxin Reagent

1998 ◽  
Vol 42 (3) ◽  
pp. 583-588 ◽  
Author(s):  
Joseph J. Drabick ◽  
Apurba K. Bhattacharjee ◽  
David L. Hoover ◽  
George E. Siber ◽  
Vivian E. Morales ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Lipid A ◽  
Polymyxin B ◽  
Human Immunoglobulin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Neutralizing Activity ◽  
Human Immunoglobulin G ◽  
Dose Dependent

ABSTRACT Polymyxin B (PMB) is a cyclic decapeptide antibiotic which also binds and neutralizes endotoxin. Unfortunately, PMB can be considerably nephrotoxic at clinically utilized doses, thereby limiting its utility as a therapeutic antiendotoxin reagent. We sought to change the pharmacokinetics and toxicity profile of PMB by covalently linking it to a human immunoglobulin G (IgG) carrier. Conjugates of PMB with IgG were prepared by EDAC [1-ethyl-3-(3-dimethylaminopropyl) carbodiimide]-mediated amide formation. Analysis by dot enzyme-linked immunosorbent assay with an anti-PMB monoclonal antibody showed that the purified conjugate contained bound PMB. The IgG-PMB conjugate reacted with lipid A and J5 lipopolysaccharide in Western blot assays in a manner comparable to that of whole antiserum with anti-lipid A reactivity; unconjugated IgG had no reactivity. The PMB bound in the conjugate retained its endotoxin-neutralizing activity compared to that of unbound PMB as evidenced by its dose-dependent inhibition of tumor necrosis factor release by endotoxin-stimulated human monocytes in vitro; unconjugated IgG had no activity. By this assay, the PMB-IgG conjugate was determined to have approximately 3.0 μg of bound functional PMB per 100 μg of total protein of conjugate (five molecules of PMB per IgG molecule). The PMB-IgG conjugate was also bactericidal against clinical strains of Escherichia coli,Pseudomonas aeruginosa, and Klebsiella pneumoniae relative to unconjugated IgG with MBCs of <4 μg of conjugate per ml for each of the tested strains. The conjugate appeared to be nontoxic at the highest doses deliverable and provided statistically significant protection from death to galactosamine-sensitized, lipopolysaccharide-challenged mice in a dose-dependent fashion when administered prophylactically 2 h before challenge. However, neither free PMB nor the PMB-IgG conjugate could protect mice challenged with endotoxin 2 h after administration. This suggests that these reagents can play a role in prophylaxis but not in therapy of sepsis. These experiments demonstrated that the PMB-IgG conjugate retains bound yet functional PMB as evidenced by its endotoxin-neutralizing activity both in vitro and in vivo. Further work is required to define the role that this or related conjugate compounds may play in the prophylaxis of endotoxin-mediated disease.

Cleavage of Human Immunoglobulin G by Treponema denticola

Anaerobe ◽  
2001 ◽  
Vol 7 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Daniel Grenier ◽  
Denis Mayrand
Keyword(s):  
Immunoglobulin G ◽  
Human Immunoglobulin ◽  
Treponema Denticola ◽  
Human Immunoglobulin G
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