Nitric Oxide Binding to Ferric Cytochrome P450:  A Computational Study

2000 ◽  
Vol 39 (11) ◽  
pp. 2352-2359 ◽  
Author(s):  
Damián A. Scherlis ◽  
Cora B. Cymeryng ◽  
Darío A. Estrin
Keyword(s):  
Nitric Oxide ◽  
Cytochrome P450 ◽  
Computational Study

scholarly journals Characterization of the Mechanism of Cytochrome P450 Reductase-Cytochrome P450-mediated Nitric Oxide and Nitrosothiol Generation from Organic Nitrates

2006 ◽  
Vol 281 (18) ◽  
pp. 12546-12554 ◽  
Author(s):  
Haitao Li ◽  
Xiaoping Liu ◽  
Hongmei Cui ◽  
Yeong-Renn Chen ◽  
Arturo J. Cardounel ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cytochrome P450 ◽  
Organic Nitrates ◽  
Cytochrome P450 Reductase ◽  
P450 Reductase

scholarly journals Nitric Oxide‐Dependent Proteasomal Degradation of Cytochrome P450 2B Proteins

2006 ◽  
Vol 20 (4) ◽  
Author(s):  
Choon‐Myung Lee ◽  
Bong‐Yoon Kim ◽  
Edward T Morgan
Keyword(s):  
Nitric Oxide ◽  
Cytochrome P450 ◽  
Proteasomal Degradation

Nitric Oxide Mediates Hepatic Cytochrome P450 Dysfunction Induced by Endotoxin

1996 ◽  
Vol 84 (6) ◽  
pp. 1435-1442 ◽  
Author(s):  
Claudia M. Muller ◽  
Annette Scierka ◽  
Richard L. Stiller ◽  
Yong-Myeong Kim ◽  
Ryan D. Cook ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cytochrome P450 ◽  
Drug Metabolism ◽  
Sprague Dawley ◽  
Cytochrome P450 Content ◽  
Nitrate Concentrations ◽  
Hypnotic Drugs ◽  
Plasma Nitrite

Background Animals subjected to immunostimulatory conditions (sepsis) exhibit decreased total cytochrome P450 content and decreased P450-dependent drug metabolism. Cytochrome P450 function is of clinical significance because it mediates the metabolism of some opioid and hypnotic drugs. The authors tested the hypothesis that reduced P450 function and decreased drug metabolism in sepsis are mediated by endotoxin-enhanced synthesis of nitric oxide. Methods Hepatic microsomes were prepared from male Sprague-Dawley rats in nontreated rats, rats pretreated with phenobarbital and rats receiving aminoguanidine or NG-L-monomethyl-arginine alone. Nitric oxide synthesis was augmented for 12 h with a single injection of bacterial lipopolysaccharides. Nitric oxide synthase was inhibited with aminoguanidine or N(G)-L-monomethyl-arginine during the 12 h of endotoxemia in some animals. Plasma nitrite and nitrate concentrations were measured in vivo, and total microsomal P450 content, and metabolism of ethylmorphine and midazolam in vitro. Results Administration of endotoxin increased plasma nitrite and nitrate concentrations, decreased total cytochrome P450 content, and decreased metabolism of ethylmorphine and midazolam. Inhibition of nitric oxide formation by aminoguanidine or N(G)-L-monomethyl-arginine partially prevented the endotoxin-induced effects in the nontreated and phenobarbital-treated groups. Aminoguanidine or N(G)-L-monomethyl-arginine alone did not have an effect on either total cytochrome P450 content or P450-dependent drug metabolism. Plasma nitrite and nitrate concentrations correlated significantly negatively with P450 content (nontreated r = -0.88, phenobarbital r = -0.91), concentrations of formed formaldehyde (nontreated r = -0.87, phenobarbital r = -0.95), and concentrations of midazolam metabolites (4-OH midazolam nontreated r = -0.88, phenobarbital r = -0.93, and 1'-OH midazolam nontreated r = -0.88, phenobarbital r = -0.97). Conclusions Altered hepatic microsomal ethylmorphine and midazolam metabolism during sepsis is mediated in large part by nitric oxide.

scholarly journals Formation of Transient Oxygen Complexes of Cytochrome P450 BM3 and Nitric Oxide Synthase under High Pressure

2003 ◽  
Vol 85 (5) ◽  
pp. 3303-3309 ◽  
Author(s):  
Stéphane Marchal ◽  
Hazel Mary Girvan ◽  
Antonius C.F. Gorren ◽  
Bernd Mayer ◽  
Andrew William Munro ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
High Pressure ◽  
Cytochrome P450 ◽  
Nitric Oxide Synthase ◽  
P450 Bm3 ◽  
Cytochrome P450 Bm3 ◽  
Oxide Synthase ◽  
Oxygen Complexes

EFFECTS OF NITRIC OXIDE ON THE METABOLIC ACTIVITY OF HEPATIC CYTOCHROME P450

1992 ◽  
Vol 77 (Supplement) ◽  
pp. A442
Author(s):  
E. Masaki ◽  
H. M. Marsh ◽  
R. A. Van Dyke
Keyword(s):  
Nitric Oxide ◽  
Cytochrome P450 ◽  
Metabolic Activity ◽  
Hepatic Cytochrome

Thermodynamic and Kinetic Studies on the Binding of Nitric Oxide to a New Enzyme Mimic of Cytochrome P450

2006 ◽  
Vol 128 (41) ◽  
pp. 13611-13624 ◽  
Author(s):  
Alicja Franke ◽  
Natalya Hessenauer-Ilicheva ◽  
Dominik Meyer ◽  
Grażyna Stochel ◽  
Wolf-D. Woggon ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cytochrome P450 ◽  
Kinetic Studies ◽  
Enzyme Mimic

Hepatic cytochrome P450 is directly inactivated by nitric oxide, not by inflammatory cytokines, in the early phase of endotoxemia

1999 ◽  
Vol 30 (6) ◽  
pp. 1035-1044 ◽  
Author(s):  
Shigekazu Takemura ◽  
Yukiko Minamiyama ◽  
Susumu Imaoka ◽  
Yoshihiko Funae ◽  
Kazuhiro Hirohashi ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cytochrome P450 ◽  
Inflammatory Cytokines ◽  
Early Phase ◽  
Hepatic Cytochrome
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