Copper Phenanthrene Oxidative Chemical Nucleases

2014 ◽  
Vol 53 (10) ◽  
pp. 5392-5404 ◽  
Author(s):  
Zara Molphy ◽  
Andreea Prisecaru ◽  
Creina Slator ◽  
Niall Barron ◽  
Malachy McCann ◽  
...  
Keyword(s):  
Chemical Nucleases

Targeted chemical nucleases have a wide range of untapped applications in biological fields, including gene therapy

2021 ◽  
Author(s):  
Moataz Dowaidar
Keyword(s):  
Metal Complex ◽  
Dna Cleavage ◽  
Therapeutic Potential ◽  
Chemical Reactivity ◽  
Major Groove ◽  
Sequence Recognition ◽  
Wide Range ◽  
Chemical Nucleases

A feasible alternative to state-of-the-art enzymatic nucleases was created by regulating the cleavage activity of metal complexes using (covalent or non-covalent) homing agents. Targeted AMNs, unlike enzymatic nucleases, break DNA by an oxidative mechanism and can therefore permanently knock off genes. Compared to larger enzymatic nucleases, the modest size of the metal complex may aid cellular transfection. Furthermore, the painstaking construction of the sequence-specific probe permits a metal complex to be directed to dsDNA's minor or major groove. To direct the chemical reactivity of several small-molecule compounds to dsDNA's minor groove, covalently bonded polyamide samples were used. PNA and DNA were also used to construct antisense and antigen hybrids, with Watson–Crick or Hoogsteen base pairing with major groove nucleobases giving sequence recognition. Click chemistry created chimeric AMN-TFOs with desirable focused effects and negligible off-target cleavage. Clip-Phen-modified TFOs, 230 polypyridyl-modified TFOs, 232 and intercalating phenanthrene-modified TFOs are three contemporary instances of copper AMN–TFOs. All three systems have distinct advantages in maintaining the desired 2:1 phenthroline/copper ratio for DNA cleavage (clip-Phen TFOs), caging the copper center and facilitating efficient ROS-mediated strand scission (polypyridyl-modified TFO) and improving triplex stability (polypyridyl-modified TFO) (phenanthrene-TFOs). Cerium (IV)/EDTA complexes, recently shown to bind and hydrolytically cleave ssDNA/dsDNA junctions and used in conjunction with PNA to successfully introduce genome changes in vitro and in vivo, are another important class of targeted chemical nucleases. The chemical reactivity and wide flexibility of metal complex design, combined with their coupling to sequence specific samples for directed applications, show that these compounds have a wide range of untapped applications in biological fields such as chemotherapy, protein engineering, DNA footprinting, and gene editing. Parallel advancements in cell and tissue targeting will be essential to maximise their therapeutic potential, either by using specific ligands or creating new targeting modalities.

Chemical nucleases

1993 ◽  
Vol 93 (6) ◽  
pp. 2295-2316 ◽  
Author(s):  
David S. Sigman ◽  
Abhijit. Mazumder ◽  
David M. Perrin
Keyword(s):  
Chemical Nucleases

New approach in the design of the chemical nucleases. The oligonucleotide derivatives of cobalt-corrin complexes.

1993 ◽  
Vol 51 (1-2) ◽  
pp. 521 ◽  
Author(s):  
G.N. Novodarova ◽  
N.Yu. Krainova ◽  
M.E. Vol'pin ◽  
V.M. Belkov ◽  
N.F. Krynetskaya ◽  
...  
Keyword(s):  
New Approach ◽  
Chemical Nucleases ◽  
Derivatives Of ◽  
Oligonucleotide Derivatives

NHC-Silver(I) Complexes as Chemical Nucleases; Synthesis, Crystal Structures, and Antibacterial Studies

2014 ◽  
Vol 25 (3) ◽  
pp. 194-204 ◽  
Author(s):  
Rosenani A. Haque ◽  
Patrick O. Asekunowo ◽  
Mohd. R. Razali ◽  
Faisal Mohamad
Keyword(s):  
Crystal Structures ◽  
Antibacterial Studies ◽  
Chemical Nucleases

ChemInform Abstract: Chemical Nucleases

ChemInform ◽  
2010 ◽  
Vol 25 (3) ◽  
pp. no-no
Author(s):  
D. S. SIGMAN ◽  
A. MAZUMDER ◽  
D. M. PERRIN
Keyword(s):  
Chemical Nucleases

Copper Complexes with Sulfonamides Derivatives of Natural Amino Acids as Chemical Nucleases

2007 ◽  
Vol 633 (11-12) ◽  
pp. 1937-1944 ◽  
Author(s):  
Benigno Macías ◽  
María V. Villa ◽  
Laura Sánchez-Mesonero ◽  
Francisca Sanz ◽  
Joaquín Borrás ◽  
...  
Keyword(s):  
Amino Acids ◽  
Copper Complexes ◽  
Chemical Nucleases ◽  
Natural Amino Acids ◽  
Derivatives Of

scholarly journals Use of site-specifically tethered chemical nucleases to study macromolecular reactions

10.1251/bpo49 ◽  
2003 ◽  
Vol 5 (1) ◽  
pp. 78-89 ◽  
Author(s):  
Srabani Mukherjee ◽  
Rui Sousa
Keyword(s):  
Chemical Nucleases
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