scholarly journals Characterization of the Zn(II) Binding Properties of the Human Wilms’ Tumor Suppressor Protein C-terminal Zinc Finger Peptide

2014 ◽  
Vol 53 (12) ◽  
pp. 6309-6320 ◽  
Author(s):  
Ka Lam Chan ◽  
Inna Bakman ◽  
Amy R. Marts ◽  
Yuksel Batir ◽  
Terry L. Dowd ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Zinc Finger ◽  
Protein C ◽  
Wilms Tumor ◽  
Tumor Suppressor Protein ◽  
Binding Properties ◽  
Zinc Finger Peptide

Characterization of RNA Aptamer Binding by the Wilms' Tumor Suppressor Protein WT1†

Biochemistry ◽  
2001 ◽  
Vol 40 (7) ◽  
pp. 2032-2040 ◽  
Author(s):  
Gary Zhai ◽  
Maya Iskandar ◽  
Kathleen Barilla ◽  
Paul J. Romaniuk
Keyword(s):  
Tumor Suppressor ◽  
Wilms Tumor ◽  
Tumor Suppressor Protein ◽  
Rna Aptamer

Characterization of Monoclonal Antibodies Directed to the Amino-Terminus of the WT1, Wilms' Tumor Suppressor Protein

Hybridoma ◽  
1998 ◽  
Vol 17 (2) ◽  
pp. 191-198 ◽  
Author(s):  
FRANK J. RAUSCHER ◽  
JENNIFER F. MORRIS ◽  
WILLIAM J. FREDERICKS ◽  
JESUS LOPEZ-GUISA ◽  
CHRISTOPHER BALAKRISHNAN ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Tumor Suppressor ◽  
Wilms Tumor ◽  
Tumor Suppressor Protein ◽  
Amino Terminus

Structure of the Wilms Tumor Suppressor Protein Zinc Finger Domain Bound to DNA

2007 ◽  
Vol 372 (5) ◽  
pp. 1227-1245 ◽  
Author(s):  
Raphael Stoll ◽  
Brian M. Lee ◽  
Erik W. Debler ◽  
John H. Laity ◽  
Ian A. Wilson ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Zinc Finger ◽  
Wilms Tumor ◽  
Tumor Suppressor Protein ◽  
Zinc Finger Domain

VRML in Cancer Research: Local Changes in Binding Properties of Wild Type and Mutated p53 Tumor Suppressor Protein

1997 ◽  
Vol 3 (9) ◽  
pp. 382-385
Author(s):  
Gerd Moeckel ◽  
Matthias Keil ◽  
Monica Hollstein ◽  
Bertold Spiegelhalder ◽  
Helmut Bartsch ◽  
...  
Keyword(s):  
Cancer Research ◽  
Tumor Suppressor ◽  
Tumor Suppressor Protein ◽  
Wild Type ◽  
Binding Properties ◽  
P53 Tumor Suppressor ◽  
P53 Tumor Suppressor Protein

DNA-binding Properties of the p53 Tumor Suppressor Protein

1994 ◽  
Vol 59 (0) ◽  
pp. 207-213 ◽  
Author(s):  
C. Prives ◽  
J. Bargonetti ◽  
G. Farmer ◽  
E. Ferrari ◽  
P. Friedlander ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Dna Binding ◽  
Tumor Suppressor Protein ◽  
Binding Properties ◽  
P53 Tumor Suppressor ◽  
P53 Tumor Suppressor Protein ◽  
Dna Binding Properties

scholarly journals Genomic characterization of Wilms' tumor suppressor 1 targets in nephron progenitor cells during kidney development

Development ◽  
2010 ◽  
Vol 137 (7) ◽  
pp. 1189-1203 ◽  
Author(s):  
S. Hartwig ◽  
J. Ho ◽  
P. Pandey ◽  
K. MacIsaac ◽  
M. Taglienti ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Progenitor Cells ◽  
Kidney Development ◽  
Wilms Tumor ◽  
Genomic Characterization ◽  
Nephron Progenitor

The Genomic Landscape of Wilms' Tumor 1 (WT1) Mutant Acute Myeloid Leukemia

Blood ◽  
2020 ◽  
Vol 136 (Supplement 1) ◽  
pp. 28-28
Author(s):  
Hassan Awada ◽  
Arda Durmaz ◽  
Carmelo Gurnari ◽  
Misam Zawit ◽  
Sunisa Kongkiatkamon ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Tumor Suppressor ◽  
Zinc Finger ◽  
Myeloid Leukemia ◽  
Wilms Tumor ◽  
Deletion Syndrome ◽  
Wt1 Gene ◽  
Genomic Landscape ◽  
Wilms Tumor 1 ◽  
Acute Myeloid

Mutations in tumor suppressor genes and oncogenes are both potentially therapeutically actionable in acute myeloid leukemia (AML). The Wilms' Tumor 1 (WT1) gene is located on 11p13 and encodes a zinc finger transcription factor which has been found to be overexpressed and mutated in AML. In normal development, WT1 is only expressed in a small subset of hematopoietic stem cells. While its overexpression suggests an oncogenic role, the invariable presence of mutations in the cysteine-histidine zinc finger domains indicates a tumor suppressor function, similar to that in WAGR syndrome/11p deletion syndrome in which it was first discovered. Like its unknown function in AML, the clinical significance and genetic associations of WT1 mutations have been also controversial. Although studies of WT1 mutations in AML have been conducted, the lack of solid clinical and molecular characterization of large WT1-mutant (WT1MT) AML cohort has hampered its definition. In this study, we took advantage of a compendia of genomic results from Cleveland Clinic and publicly available data of 2188 AML patients (primary (p)AML, n= 1636; secondary (s)AML, n= 433; therapy-related (t)AML, n= 119, excluding cases with acute promyelocytic leukemia, MLL-rearrangement, and core-binding factor AML). While several reports only focused on cytogenetic normal AML (CN-AML), which represented 61% of our cohort, we additionally included all other cytogenetic risk groups. In total, WT1 mutations were detected in 5% (114/2188) of patients. WT1 mutations were enriched in pAML (85%) compared to sAML (11%) and tAML (4%). Thirty-nine patients (13%) carried more than 1 WT1 mutation. WT1MT were younger [59 vs 64 years, P=0.0002] and more often females (55% vs 45%, P=0.03) as compared to WT1 wild type (WT1WT) patients. Univariate analyses of baseline parameters showed that WT1MT AML had a more proliferative phenotype with a higher WBC [15.1 vs 9.5 x109/L, P=0.03] and bone marrow blast percentages [73 vs 59%, P=0.002] and with lower platelet counts [44 vs 56 x109/L, P=0.008] compared to WT1WT cases. In the WT1MT cohort, 70% had a normal karyotype, with complex karyotype being significantly less frequent vsWT1WT patients [4 vs 16%, P=0.001]. The most common cytogenetic abnormalities in WT1MT patients included +8 (8%) followed by -9/del(9q) (3%) and -7/del(7q) (3%). Only 1 patient carried inv(3)/t(3;3) or -17/del(17p). In sum, no statistical differences in cytogenetics were found between WT1MTvsWT1WT AML patients. Next, identified mutational signatures of WT1MT patients. A panel of 44 myeloid genes and their hotspot configurations were selected according to their relevance in AML. In comparison to WT1WT AML patients, multivariate analyses showed that WT1MT patients had higher odds of biallelic CEBPA (12 vs 3%; P=0.009) and FLT3 internal tandem duplication mutations (FLT3ITD, 31 vs 16%; P=0.01) but lower odds of SRSF2 mutations (2 vs 9%, P=0.04). Since FLT3ITD has been previously described to be associated with WT1 mutations, we also focused on investigating whether mutations in the tyrosine kinase domain (TKD) were frequent in WT1MT as well. Although we found increased percentages of FLT3TKD (11%) among the WT1MT patients compared to WT1WT cohort (8%), this difference did not reach statistical significance. To uncover multifactor lesions (cytogenetic and/ or additional molecular lesions) of prognostic importance, we performed survival analyses. Although the combination of WT1 mutations and FLT3TKD shortened overall survival (OS) by 2-times in WT1MT patients vsWT1WT cases with FLT3TKD (23.7 vs 45.9 months), this result was not significant (P=0.1). In addition, the concurrent presence of other cytogenetic and molecular features didn't reveal significant impact on OS. In sum, using an adequately powered cohort, our study of the genomic landscape of WT1MT AML patients identified its genomic associations and their clinical and prognostic inferences. The application of advanced machine learning methods to large datasets of WT1MT AML patients might be crucial to capture the complex genomic interactions of WT1 gene in AML. Disclosures Carraway: BMS: Consultancy, Other: Research support, Speakers Bureau; Stemline: Consultancy, Speakers Bureau; Takeda: Other: Independent Advisory Committe (IRC); ASTEX: Other: Independent Advisory Committe (IRC); Abbvie: Other: Independent Advisory Committe (IRC); Novartis: Consultancy, Speakers Bureau; Jazz: Consultancy, Speakers Bureau. Nazha:MEI: Other: Data monitoring Committee; Novartis: Speakers Bureau; Incyte: Speakers Bureau; Jazz: Research Funding. Sekeres:Pfizer: Consultancy; BMS: Consultancy; Takeda/Millenium: Consultancy. Maciejewski:Alexion, BMS: Speakers Bureau; Novartis, Roche: Consultancy, Honoraria.

scholarly journals Functional characterization of Wilms tumor-suppressor WTX and tumor-associated mutants

Oncogene ◽  
2010 ◽  
Vol 30 (7) ◽  
pp. 832-842 ◽  
Author(s):  
M K-H Kim ◽  
D J Min ◽  
M Rabin ◽  
J D Licht
Keyword(s):  
Tumor Suppressor ◽  
Wilms Tumor ◽  
Functional Characterization
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