scholarly journals Dipeptide-Based Models of Nickel Superoxide Dismutase: Solvent Effects Highlight a Critical Role to Ni–S Bonding and Active Site Stabilization

2011 ◽  
Vol 50 (20) ◽  
pp. 10460-10471 ◽  
Author(s):  
Eric M. Gale ◽  
Darin M. Cowart ◽  
Robert A. Scott ◽  
Todd C. Harrop
Keyword(s):  
Superoxide Dismutase ◽  
Solvent Effects ◽  
Active Site ◽  
Critical Role ◽  
Nickel Superoxide Dismutase

Synthesis and Characterization of NiIIIN3S2 Complexes as Active Site Models for the Oxidized Form of Nickel Superoxide Dismutase

2014 ◽  
Vol 20 (21) ◽  
pp. 6283-6286 ◽  
Author(s):  
Chien-Wei Chiang ◽  
Yun-Li Chu ◽  
Hong-Ling Chen ◽  
Ting-Shen Kuo ◽  
Way-Zen Lee
Keyword(s):  
Superoxide Dismutase ◽  
Active Site ◽  
Synthesis And Characterization ◽  
Nickel Superoxide Dismutase

scholarly journals Stepwise maturation of the peptidyl transferase region of human mitoribosomes

2021 ◽  
Author(s):  
Tea Lenarcic ◽  
Mateusz Jaskolowski ◽  
Marc Leibundgut ◽  
Alain Scaiola ◽  
Tanja Schoenhut ◽  
...  
Keyword(s):  
Quality Control ◽  
16S Rrna ◽  
Active Site ◽  
Critical Role ◽  
Ribosomal Subunit ◽  
Structural Basis ◽  
Ribosome Assembly ◽  
Peptidyl Transferase ◽  
Mitochondrial Ribosomes ◽  
Mitochondrial Ribosome

Mitochondrial ribosomes are specialized for the synthesis of membrane proteins responsible for oxidative phosphorylation. Mammalian mitoribosomes diverged considerably from the ancestral bacterial ribosomes and feature dramatically reduced ribosomal RNAs. Structural basis of the mammalian mitochondrial ribosome assembly is currently not understood. Here we present eight distinct assembly intermediates of the human large mitoribosomal subunit involving 7 assembly factors. We discover that NSUN4-MTERF4 dimer plays a critical role in the process by stabilizing the 16S rRNA in a conformation that exposes the functionally important regions of rRNA for modification by MRM2 methyltransferase and quality control interactions with a conserved mitochondrial GTPase MTG2 that contacts the sarcin ricin loop and the immature active site. The successive action of these factors leads to the formation of the peptidyl transferase active site of the mitoribosome and the folding of the surrounding rRNA regions responsible for interactions with tRNAs and the small ribosomal subunit.

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