Determination of Aromatics in Light Petroleum Distillates By Use of Specific Dispersions between Sodium D Line and Mercury g Line

1945 ◽  
Vol 17 (8) ◽  
pp. 481-486 ◽  
Author(s):  
H.M. Thorne ◽  
Walter Murphy ◽  
John S. Ball
Keyword(s):  
Light Petroleum ◽  
Petroleum Distillates

Simultaneous determination of five sex hormones in human serum by radioimmunoassay after chromatography on Lipidex-5000.

1976 ◽  
Vol 22 (1) ◽  
pp. 32-38 ◽  
Author(s):  
D Apter ◽  
O Jánne ◽  
P Karvonen ◽  
R Vihko
Keyword(s):  
Menstrual Cycle ◽  
Human Serum ◽  
Simultaneous Determination ◽  
Sex Hormones ◽  
Oral Contraceptives ◽  
Light Petroleum ◽  
Serum Concentrations ◽  
Coefficients Of Variation ◽  
Normal Men

Abstract We describe a method for determination of pregnenolone, progesterone, 17alpha-hydroxyprogesterone, testosterone, and 5alpha-dihydrotestosterone in 1-2 ml of serum from male or female. Using microcolumns of Lipidex-5000 (hydroxyalkoxypropyl Sephadex, 0.5 g) and light petroleum/chloroform (97/3) as the solvent during chromatography, we resolved these five steroids into four fractions, with pregnenolone and 5alpha-dihydrotestosterone eluting together. By use of selected antibodies, the latter two steroids were also determined specifically. Use of microcolumns allowed minimization of solvent volumes and sample transfers. Consequently, blank values for all the five steroids were negligible. Lowest measureable concentrations (in ng/liter) were: pregnenolone 100, progesterone 25, 17alpha-hydroxyprogesterone 50, testosterone 25, and 5alpha-dihydrotestosterone 25. Intra-assay and inter-assay coefficients of variation ranged from 5 to 9% and 10 to 15%, respectively, for the five steroids. Serum concentrations of these steroids are given for women in the follicular and luteal phases of the menstrual cycle and for women on oral contraceptives of the combination type, as well as for normal men.

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