Determination of Vitamin A and Carotenoids in Butterfat. Comparison of Direct Spectrophotometry with Filter Photometry and Use of the Antimony Trichloride Reaction

1944 ◽  
Vol 16 (2) ◽  
pp. 83-85 ◽  
Author(s):  
F P. ZSCHEILE ◽  
H A. NASH ◽  
R L. HENRY ◽  
L F. GREEN
Keyword(s):  
Vitamin A ◽  
Antimony Trichloride ◽  
Direct Spectrophotometry ◽  
Filter Photometry

Determination of Vitamins D2 and D3 in Pharmaceuticals by Gas-Liquid Chromatography

1968 ◽  
Vol 51 (4) ◽  
pp. 839-840
Author(s):  
T K Murray ◽  
P Erdody ◽  
T Panalaks
Keyword(s):  
Vitamin D ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
Coefficient Of Variation ◽  
Internal Standard ◽  
Antimony Trichloride ◽  
The Other ◽  
Gas Liquid Chromatography ◽  
Partition Chromatography

Abstract A method is described for determining vitamin D in multivitamin preparations; in the method, vitamins D2 and D3 are isomerized with antimony trichloride and separated by GLC. Vitamins D,2 and D3 are differentiated and measured separately and one vitamin may be used as an internal standard for the other. Vitamin A is largely removed by partition chromatography but can be tolerated in the final dilution in a ratio of 1:1 with vitamin D. When the method was used for the assay of multivitamin preparations, the coefficient of variation was 3.2%.

THE ANTIMONY TRICHLORIDE METHOD FOR THE DETERMINATION OF VITAMIN A

1944 ◽  
Vol 22b (2) ◽  
pp. 21-31 ◽  
Author(s):  
G. H. Benham
Keyword(s):  
Vitamin A ◽  
Petroleum Ether ◽  
Ethyl Ether ◽  
Soap Solution ◽  
Antimony Trichloride ◽  
Chemical Test ◽  
The Absolute

A critical description of the antimony trichloride method for the determination of vitamin A is presented. Low values for vitamin A result from:(1) Incomplete extraction from the alcoholic soap solution by using petroleum ether instead of ethyl ether,(2) Incomplete separation of the layers during extraction and washing,(3) Incomplete filtration through anhydrous sodium sulphate.If strict attention is paid to details of procedure, the method gives consistent and reproducible results. Uncertainty in regard to the exact factor for converting the E values to international units makes it impossible at this time to state accurately the absolute values. It is pointed out that this in no way detracts from the usefulness of the chemical test.

Vitamin A Value of Plant Food Provitamin A - Evaluated by the Stable Isotope Technologies

2014 ◽  
Vol 84 (Supplement 1) ◽  
pp. 25-29 ◽  
Author(s):  
Guangwen Tang
Keyword(s):  
Stable Isotope ◽  
Vitamin A ◽  
Provitamin A ◽  
Animal Origin ◽  
Plant Food ◽  
Plant Foods ◽  
A Value ◽  
Provitamin A Carotenoids ◽  
Β Carotene

Humans need vitamin A and obtain essential vitamin A by conversion of plant foods rich in provitamin A and/or absorption of preformed vitamin A from foods of animal origin. The determination of the vitamin A value of plant foods rich in provitamin A is important but has challenges. The aim of this paper is to review the progress over last 80 years following the discovery on the conversion of β-carotene to vitamin A and the various techniques including stable isotope technologies that have been developed to determine vitamin A values of plant provitamin A (mainly β-carotene). These include applications from using radioactive β-carotene and vitamin A, depletion-repletion with vitamin A and β-carotene, and measuring postprandial chylomicron fractions after feeding a β-carotene rich diet, to using stable isotopes as tracers to follow the absorption and conversion of plant food provitamin A carotenoids (mainly β-carotene) in humans. These approaches have greatly promoted our understanding of the absorption and conversion of β-carotene to vitamin A. Stable isotope labeled plant foods are useful for determining the overall bioavailability of provitamin A carotenoids from specific foods. Locally obtained plant foods can provide vitamin A and prevent deficiency of vitamin A, a remaining worldwide concern.

scholarly journals OBSERVATIONS BEARING ON THE DETERMINATION OF VITAMIN A

1928 ◽  
Vol 80 (1) ◽  
pp. 215-226
Author(s):  
E.M. Nelson ◽  
D.Breese Jones
Keyword(s):  
Vitamin A

Application of the antimony trichloride-spectrophotometric method to the determination of gossypol in cottonseed and cottonseed products

1948 ◽  
Vol 25 (12) ◽  
pp. 457-461 ◽  
Author(s):  
Catherine M. Hall ◽  
Leah E. Castillon ◽  
Wilma A. Guice ◽  
Charlotte H. Boatner
Keyword(s):  
Spectrophotometric Method ◽  
Antimony Trichloride
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