New Method for the Direct Determination of Dissolved Fe(III) Concentration in Acid Mine Waters

1999 ◽  
Vol 33 (5) ◽  
pp. 807-813 ◽  
Author(s):  
Tanya Bangthanh To ◽  
D. Kirk Nordstrom ◽  
Kirkwood M. Cunningham ◽  
James W. Ball ◽  
R. Blaine McCleskey
Keyword(s):  
Direct Determination ◽  
New Method ◽  
Mine Waters ◽  
Acid Mine ◽  
Acid Mine Waters

A New Approach to Quantify Na-Fluorescein (Uranine) in Acid Mine Waters

2011 ◽  
Vol 30 (3) ◽  
pp. 231-236 ◽  
Author(s):  
Lara Naurath ◽  
Christoph Weidner ◽  
Thomas R. Rüde ◽  
Andre Banning
Keyword(s):  
Mine Waters ◽  
New Approach ◽  
Acid Mine ◽  
Acid Mine Waters

scholarly journals Leptin Binding and Binding Capacity in Serum

2000 ◽  
Vol 46 (3) ◽  
pp. 379-384 ◽  
Author(s):  
Michael Landt
Keyword(s):  
Adipose Tissue ◽  
Molecular Mass ◽  
Binding Capacity ◽  
Direct Determination ◽  
New Method ◽  
Degree Of Saturation ◽  
Free Form ◽  
Hplc Separation ◽  
Serum Leptin

Abstract Background: Leptin, a hormone produced primarily by adipose tissue, is known to be present in serum as both monomeric (free) and higher molecular mass (bound) forms, but little is known about the nature of the bound forms or physiological variation in binding capacity. Methods: A new method to quantify the free and bound forms was developed, based on HPLC separation and RIA quantification in chromatography fractions. Reanalysis of specimens after addition of exogenous leptin allowed direct determination of leptin-binding capacity and the degree of saturation of leptin-binding capacity. Results: HPLC chromatography fractionated serum leptin into both the free form and as a broad peak of 59–130 kDa. Several experiments were conducted to validate the new method. The concentrations of bound leptin in serum were 0.45–3.94 μg/L, and they increased as total leptin (reflecting adiposity) increased in 24 lean and obese volunteers. Leptin was readily dissociated from the bound fraction by competition from exogenous leptin. Rechromatography of the bound fraction led to dissociation of leptin, which was promoted by warming the sera before chromatography. Leptin-binding capacity was 1.8–5.3 μg/L; binding capacity was nearly constant over a range of total leptin concentrations of 2–10 μg/L, and slowly increased at higher total leptin concentrations. Saturation of binding capacity was low (15%) at very low total leptin concentrations (<5 μg/L), but rose quickly to a plateau near 80% at higher total leptin concentrations. Conclusions: The new method facilitates measurement of free and bound fractions of serum leptin, and is the first method measuring leptin-binding capacity. These experiments demonstrate that the concentration of bound leptin and leptin-binding capacity vary physiologically, with binding/binding capacity increasing with adiposity. Except in very lean individuals, binding capacity is nearly completely saturated.

scholarly journals Comparison of acid-resistant ceramic and polymeric nanofiltration membranes for acid mine waters treatment

2020 ◽  
Vol 382 ◽  
pp. 122786 ◽  
Author(s):  
J. López ◽  
M. Reig ◽  
X. Vecino ◽  
O. Gibert ◽  
J.L. Cortina
Keyword(s):  
Nanofiltration Membranes ◽  
Mine Waters ◽  
Acid Mine ◽  
Acid Mine Waters ◽  
Resistant Ceramic
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