Evaluation of Possible Causes for the Decline of Japanese Cedar (Cryptomeria japonica) Based on Elemental Composition and δ13C of Needles

1996 ◽  
Vol 30 (7) ◽  
pp. 2376-2381 ◽  
Author(s):  
Masahiro Sakata
Keyword(s):  
Elemental Composition ◽  
Cryptomeria Japonica ◽  
Japanese Cedar

Variation in amount and elemental composition of epicuticular wax in Japanese cedar (Cryptomeria japonica) leaves associated with natural environmental factors

1998 ◽  
Vol 28 (1) ◽  
pp. 87-97 ◽  
Author(s):  
Hiroyuki Sase ◽  
Takejiro Takamatsu ◽  
Tomio Yoshida
Keyword(s):  
Water Stress ◽  
Environmental Factors ◽  
Elemental Composition ◽  
Cryptomeria Japonica ◽  
Leaf Age ◽  
Japanese Cedar ◽  
Epicuticular Wax ◽  
Acidic Gases ◽  
Unit Leaf ◽  
Opposite Change

Leaf samples of Cryptomeria japonica D. Don (and some other conifers) taken from various locations in Japan were analyzed for differences in the amount and elemental composition of their epicuticular wax. In C. japonica the amount of wax per unit leaf mass was lower, and the C content of the wax relatively higher, than those of other species. The properties of the wax (amount, C and O contents) varied according to natural environmental factors such as altitude and exposure to volcanic acidic gases such as H2S, as well as branch height and leaf age within the tree. The amount of wax increased with leaf age (during the growing phase), altitude, branch height, and exposure to the gases, while the C content of wax decreased and the O content increased, except in the case of altitude, where they showed an opposite change. These findings suggest different alterations in wax properties under the effects of water stress (on high branches and at locations with a low rain factor), exposure to noxious gases, and strong UV radiation at high altitude.

scholarly journals CRISPR/Cas9-mediated targeted mutagenesis in Japanese cedar (Cryptomeria japonica D. Don)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yoshihiko Nanasato ◽  
Masafumi Mikami ◽  
Norihiro Futamura ◽  
Masaki Endo ◽  
Mitsuru Nishiguchi ◽  
...  
Keyword(s):  
Genome Editing ◽  
Cryptomeria Japonica ◽  
Fluorescent Protein ◽  
Japanese Cedar ◽  
Targeted Mutagenesis ◽  
Embryogenic Tissue ◽  
Breeding Period ◽  
Single Mutation ◽  
Knock Out ◽  
Guide Rna

AbstractCryptomeria japonica (Japanese cedar or sugi) is one of the most important coniferous tree species in Japan and breeding programs for this species have been launched since 1950s. Genome editing technology can be used to shorten the breeding period. In this study, we performed targeted mutagenesis using the CRISPR/Cas9 system in C. japonica. First, the CRISPR/Cas9 system was tested using green fluorescent protein (GFP)-expressing transgenic embryogenic tissue lines. Knock-out efficiency of GFP ranged from 3.1 to 41.4% depending on U6 promoters and target sequences. The GFP knock-out region was mottled in many lines, indicating genome editing in individual cells. However, in 101 of 102 mutated individuals (> 99%) from 6 GFP knock-out lines, embryos had a single mutation pattern. Next, we knocked out the endogenous C. japonica magnesium chelatase subunit I (CjChlI) gene using two guide RNA targets. Green, pale green, and albino phenotypes were obtained in the gene-edited cell lines. Sequence analysis revealed random deletions, insertions, and replacements in the target region. Thus, targeted mutagenesis using the CRISPR/Cas9 system can be used to modify the C. japonica genome.

scholarly journals Japanese Cedar (Cryptomeria japonica) Pollinosis in Jeju, Korea: Is It Increasing?

2015 ◽  
Vol 7 (3) ◽  
pp. 295 ◽  
Author(s):  
Jaechun Lee ◽  
Keun Hwa Lee ◽  
Hye-Sook Lee ◽  
Sung-Chul Hong ◽  
Jeong Hong Kim
Keyword(s):  
Cryptomeria Japonica ◽  
Japanese Cedar

Spatiotemporal variation in N2O flux within a slope in a Japanese cedar (Cryptomeria japonica) forest

2009 ◽  
Vol 96 (1-3) ◽  
pp. 163-175 ◽  
Author(s):  
Kazuya Nishina ◽  
Chisato Takenaka ◽  
Shigehiro Ishizuka
Keyword(s):  
Cryptomeria Japonica ◽  
Japanese Cedar ◽  
Spatiotemporal Variation ◽  
N2o Flux
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