scholarly journals Influence of Heterogeneous Ammonium Availability on Bacterial Community Structure and the Expression of Nitrogen Fixation and Ammonium Transporter Genes during in Situ Bioremediation of Uranium-Contaminated Groundwater

2009 ◽  
Vol 43 (12) ◽  
pp. 4386-4392 ◽  
Author(s):  
Paula J. Mouser ◽  
A. Lucie N’Guessan ◽  
Hila Elifantz ◽  
Dawn E. Holmes ◽  
Kenneth H. Williams ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Ammonium Transporter ◽  
Contaminated Groundwater ◽  
In Situ Bioremediation

Assessment of in situ degradation of chlorinated ethenes and bacterial community structure in a complex contaminated groundwater system

2008 ◽  
Vol 42 (4-5) ◽  
pp. 871-882 ◽  
Author(s):  
Gwenaël Imfeld ◽  
Ivonne Nijenhuis ◽  
Marcell Nikolausz ◽  
Simone Zeiger ◽  
Heidrun Paschke ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Chlorinated Ethenes ◽  
Contaminated Groundwater ◽  
Groundwater System ◽  
In Situ Degradation

Analysis of bacterial community structure in bulk soil by in situ hybridization

1997 ◽  
Vol 168 (3) ◽  
pp. 185-192 ◽  
Author(s):  
Boris Zarda ◽  
D. Hahn ◽  
Antonis Chatzinotas ◽  
Wilhelm Schönhuber ◽  
Alexander Neef ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Bulk Soil

scholarly journals Microarray and Real-Time PCR Analyses of the Responses of High-Arctic Soil Bacteria to Hydrocarbon Pollution and Bioremediation Treatments

2009 ◽  
Vol 75 (19) ◽  
pp. 6258-6267 ◽  
Author(s):  
Etienne Yergeau ◽  
Mélanie Arbour ◽  
Roland Brousseau ◽  
David Juck ◽  
John R. Lawrence ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
High Arctic ◽  
Soil Microbiology ◽  
Ex Situ ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Pcr Targeting

ABSTRACT High-Arctic soils have low nutrient availability, low moisture content, and very low temperatures and, as such, they pose a particular problem in terms of hydrocarbon bioremediation. An in-depth knowledge of the microbiology involved in this process is likely to be crucial to understand and optimize the factors most influencing bioremediation. Here, we compared two distinct large-scale field bioremediation experiments, located at the Canadian high-Arctic stations of Alert (ex situ approach) and Eureka (in situ approach). Bacterial community structure and function were assessed using microarrays targeting the 16S rRNA genes of bacteria found in cold environments and hydrocarbon degradation genes as well as quantitative reverse transcriptase PCR targeting key functional genes. The results indicated a large difference between sampling sites in terms of both soil microbiology and decontamination rates. A rapid reorganization of the bacterial community structure and functional potential as well as rapid increases in the expression of alkane monooxygenases and polyaromatic hydrocarbon-ring-hydroxylating dioxygenases were observed 1 month after the bioremediation treatment commenced in the Alert soils. In contrast, no clear changes in community structure were observed in Eureka soils, while key gene expression increased after a relatively long lag period (1 year). Such discrepancies are likely caused by differences in bioremediation treatments (i.e., ex situ versus in situ), weathering of the hydrocarbons, indigenous microbial communities, and environmental factors such as soil humidity and temperature. In addition, this study demonstrates the value of molecular tools for the monitoring of polar bacteria and their associated functions during bioremediation.

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