Particle-Fluorescence Spectrometer for Real-Time Single-Particle Measurements of Atmospheric Organic Carbon and Biological Aerosol

2009 ◽  
Vol 43 (2) ◽  
pp. 429-434 ◽  
Author(s):  
Yong-Le Pan ◽  
Ronald G. Pinnick ◽  
Steven C. Hill ◽  
Richard K. Chang
Keyword(s):  
Organic Carbon ◽  
Real Time ◽  
Single Particle ◽  
Particle Measurements ◽  
Fluorescence Spectrometer

scholarly journals Quantification of aerosol chemical composition using continuous single particle measurements

2011 ◽  
Vol 11 (1) ◽  
pp. 1219-1264 ◽  
Author(s):  
C.-H. Jeong ◽  
M. L. McGuire ◽  
K. J. Godri ◽  
J. G. Slowik ◽  
P. J. G. Rehbein ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Organic Carbon ◽  
Mass Spectrometer ◽  
Single Particle ◽  
Elemental Carbon ◽  
Rural Site ◽  
Mass Composition ◽  
Chemical Components ◽  
Particle Measurements ◽  
Mass Concentrations

Abstract. Mass concentrations of particulate matter (PM) chemical components were determined from data for 0.3 to 3.0 μm particles measured by an Aerosol Time-of-Flight Mass Spectrometer (ATOFMS) data at an urban and rural site. Hourly-averaged concentrations of nitrate, sulphate, ammonium, organic carbon, and elemental carbon, estimated based on scaled ATOFMS peak intensities of corresponding ion marker species, were compared with collocated chemical composition measurements by an Aerosol Mass Spectrometer (AMS), a Gas-Particle Ion Chromatograph (GPIC), and a Sunset Lab field OCEC analyzer. The highest correlation was found for nitrate, with correlation coefficients (Pearson r) of 0.89 and 0.85 at the urban and rural sites, respectively. ATOFMS mass calibration factors, determined for the urban site, were used to calculate mass concentrations of the major PM chemical components at the rural site. Mass reconstruction using this ATOFMS based composition data agreed very well with the total PM mass measured at the rural site. Size distributions of the ten main types of particles were resolved for the rural site and the mass composition of each particle type was determined in terms of sulphate, nitrate, ammonium, organic carbon and elemental carbon. This is the first study to estimate hourly mass concentrations of individual aerosol components and the mass composition of individual particle-types based on ATOFMS single particle measurements.

Real-time, single-particle measurements of ambient aerosols in Shanghai

2010 ◽  
Vol 5 (3) ◽  
pp. 331-341 ◽  
Author(s):  
Fan Yang ◽  
Xinning Wang ◽  
Yaping Zhang ◽  
Xiaofei Wang ◽  
Hong Chen ◽  
...  
Keyword(s):  
Real Time ◽  
Single Particle ◽  
Ambient Aerosols ◽  
Particle Measurements

Real-Time, Single-Particle Measurements of Oligomers in Aged Ambient Aerosol Particles

2007 ◽  
Vol 41 (15) ◽  
pp. 5439-5446 ◽  
Author(s):  
Kerri A. Denkenberger ◽  
Ryan C. Moffet ◽  
John C. Holecek ◽  
Thomas P. Rebotier ◽  
Kimberly A. Prather
Keyword(s):  
Real Time ◽  
Single Particle ◽  
Aerosol Particles ◽  
Ambient Aerosol ◽  
Particle Measurements

scholarly journals Real-Time 3D Single Particle Tracking: Towards Active Feedback Single Molecule Spectroscopy in Live Cells

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2826 ◽  
Author(s):  
Shangguo Hou ◽  
Courtney Johnson ◽  
Kevin Welsher
Keyword(s):  
Fluorescence Spectroscopy ◽  
Real Time ◽  
Temporal Resolution ◽  
Single Molecule ◽  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Single Molecule Fluorescence ◽  
Single Molecule Fluorescence Spectroscopy ◽  
Active Feedback

Single molecule fluorescence spectroscopy has been largely implemented using methods which require tethering of molecules to a substrate in order to make high temporal resolution measurements. However, the act of tethering a molecule requires that the molecule be removed from its environment. This is especially perturbative when measuring biomolecules such as enzymes, which may rely on the non-equilibrium and crowded cellular environment for normal function. A method which may be able to un-tether single molecule fluorescence spectroscopy is real-time 3D single particle tracking (RT-3D-SPT). RT-3D-SPT uses active feedback to effectively lock-on to freely diffusing particles so they can be measured continuously with up to photon-limited temporal resolution over large axial ranges. This review gives an overview of the various active feedback 3D single particle tracking methods, highlighting specialized detection and excitation schemes which enable high-speed real-time tracking. Furthermore, the combination of these active feedback methods with simultaneous live-cell imaging is discussed. Finally, the successes in real-time 3D single molecule tracking (RT-3D-SMT) thus far and the roadmap going forward for this promising family of techniques are discussed.

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