Characterization of Australian crudes and condensates by gas chromatographic analysis. Comments

1976 ◽  
Vol 10 (3) ◽  
pp. 284-284
Author(s):  
E. R. Adlard
Keyword(s):  
Chromatographic Analysis ◽  
Gas Chromatographic Analysis

Isolation and partial characterization of Bacillus megaterium mutants deficient in poly(3-hydroxybutyrate) synthesis

1995 ◽  
Vol 41 (13) ◽  
pp. 77-79 ◽  
Author(s):  
Mirtha E. Floccari ◽  
Nancy I. López ◽  
Beatriz S. Méndez ◽  
Ursula Pieper Fürst ◽  
Alexander Steinbüchel
Keyword(s):  
Bacillus Megaterium ◽  
Allyl Alcohol ◽  
Chromatographic Analysis ◽  
Partial Characterization ◽  
Enzymatic Characterization ◽  
Gas Chromatographic Analysis ◽  
Selection For ◽  
Coa Reductase ◽  
Acetoacetyl Coa Reductase

Poly(3-hydroxybutyrate) (PHB)-negative mutants of Bacillus megaterium were isolated following mutagenesis with N-methyl-N′-nitro-N-nitrosoguanidine. Different strategies were used for isolation, including ultracentrifugation through sucrose gradients and selection for resistance to allyl alcohol. The mutants were detected on agar plates by staining the colonies with Sudan Black. Four mutants did not synthesize any PHB as revealed by gas chromatographic analysis. The enzymatic characterization showed no or extremely low synthase activity for all of the mutants. In contrast, no significant alterations were observed in the β-ketothiolase and the NADPH-dependent acetoacetyl-CoA reductase activities. All mutants sporulated in complete and minimal media.Key words: poly(3-hydroxybutyrate), synthase, mutants, Bacillus megaterium.

Isolation and characterization of 3-deoxypentulose and its determination in heated milk

1992 ◽  
Vol 59 (4) ◽  
pp. 507-515 ◽  
Author(s):  
Esperanza Troyano ◽  
Agustin Olano ◽  
Maria Luisa Jimeno ◽  
Jesus Sanz ◽  
Isabel Martínez-Castro
Keyword(s):  
Chromatographic Analysis ◽  
Alkaline Solutions ◽  
Milk Samples ◽  
Gas Chromatographic Analysis ◽  
Isolation And Characterization ◽  
Heated Milk ◽  
Sterilized Milk ◽  
Two Peaks

SummaryThe presence of 3-deoxypentulose in heated milk is reported. This compound has been isolated from alkaline solutions of lactose and characterized by spectroscopic and Chromatographie techniques. Gas Chromatographic analysis of free monosaccharides of milk revealed two peaks corresponding to 3-deoxypentulose in commercial sterilized milk samples, in a concentration range of 30–50 mg/1. Its concentration increased with temperature and length of time of processing.

Sign in / Sign up

Export Citation Format

Share Document