scholarly journals Iron Nanoparticle-Induced Activation of Plasma Membrane H+-ATPase Promotes Stomatal Opening in Arabidopsis thaliana

2014 ◽  
Vol 49 (2) ◽  
pp. 1113-1119 ◽  
Author(s):  
Jae-Hwan Kim ◽  
Youngjun Oh ◽  
Hakwon Yoon ◽  
Inhwan Hwang ◽  
Yoon-Seok Chang
Keyword(s):  
Arabidopsis Thaliana ◽  
Plasma Membrane ◽  
Stomatal Opening ◽  
Iron Nanoparticle

scholarly journals A family of pathogen-induced cysteine-rich transmembrane proteins is involved in plant disease resistance

Planta ◽  
2021 ◽  
Vol 253 (5) ◽  
Author(s):  
Marciel Pereira Mendes ◽  
Richard Hickman ◽  
Marcel C. Van Verk ◽  
Nicole M. Nieuwendijk ◽  
Anja Reinstädler ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Plasma Membrane ◽  
Disease Resistance ◽  
Plant Disease Resistance ◽  
Transmembrane Proteins ◽  
Series Data ◽  
Immune Gene ◽  
Biological Processes ◽  
Hypocotyl Growth ◽  
Enhanced Resistance

Abstract Main conclusion Overexpression of pathogen-induced cysteine-rich transmembrane proteins (PCMs) in Arabidopsis thaliana enhances resistance against biotrophic pathogens and stimulates hypocotyl growth, suggesting a potential role for PCMs in connecting both biological processes. Abstract Plants possess a sophisticated immune system to protect themselves against pathogen attack. The defense hormone salicylic acid (SA) is an important player in the plant immune gene regulatory network. Using RNA-seq time series data of Arabidopsis thaliana leaves treated with SA, we identified a largely uncharacterized SA-responsive gene family of eight members that are all activated in response to various pathogens or their immune elicitors and encode small proteins with cysteine-rich transmembrane domains. Based on their nucleotide similarity and chromosomal position, the designated Pathogen-induced Cysteine-rich transMembrane protein (PCM) genes were subdivided into three subgroups consisting of PCM1-3 (subgroup I), PCM4-6 (subgroup II), and PCM7-8 (subgroup III). Of the PCM genes, only PCM4 (also known as PCC1) has previously been implicated in plant immunity. Transient expression assays in Nicotiana benthamiana indicated that most PCM proteins localize to the plasma membrane. Ectopic overexpression of the PCMs in Arabidopsis thaliana resulted in all eight cases in enhanced resistance against the biotrophic oomycete pathogen Hyaloperonospora arabidopsidis Noco2. Additionally, overexpression of PCM subgroup I genes conferred enhanced resistance to the hemi-biotrophic bacterial pathogen Pseudomonas syringae pv. tomato DC3000. The PCM-overexpression lines were found to be also affected in the expression of genes related to light signaling and development, and accordingly, PCM-overexpressing seedlings displayed elongated hypocotyl growth. These results point to a function of PCMs in both disease resistance and photomorphogenesis, connecting both biological processes, possibly via effects on membrane structure or activity of interacting proteins at the plasma membrane.

scholarly journals The Arabidopsis thaliana plasma membrane H(+)-ATPase multigene family. Genomic sequence and expression of a third isoform.

1990 ◽  
Vol 265 (23) ◽  
pp. 13601-13608
Author(s):  
J.F. Harper ◽  
L. Manney ◽  
N.D. DeWitt ◽  
M.H. Yoo ◽  
M.R. Sussman
Keyword(s):  
Arabidopsis Thaliana ◽  
Plasma Membrane ◽  
Multigene Family ◽  
Genomic Sequence

AIR12, a b -type cytochrome of the plasma membrane of Arabidopsis thaliana is a negative regulator of resistance against Botrytis cinerea

Plant Science ◽  
2015 ◽  
Vol 233 ◽  
pp. 32-43 ◽  
Author(s):  
Alex Costa ◽  
Maria Raffaella Barbaro ◽  
Francesca Sicilia ◽  
Valeria Preger ◽  
Anja Krieger-Liszkay ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Plasma Membrane ◽  
Botrytis Cinerea ◽  
Negative Regulator

scholarly journals Overexpression of Plasma Membrane H+-ATPase in Guard Cells Enhances Light-Induced Stomatal Opening, Photosynthesis, and Plant Growth in Hybrid Aspen

2021 ◽  
Vol 12 ◽  
Author(s):  
Shigeo Toh ◽  
Naoki Takata ◽  
Eigo Ando ◽  
Yosuke Toda ◽  
Yin Wang ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Transgenic Plants ◽  
Woody Plants ◽  
Stem Elongation ◽  
Guard Cells ◽  
Gus Expression ◽  
Stomatal Opening ◽  
Wild Type ◽  
Exchange System ◽  
Gas Exchange System

Stomata in the plant epidermis open in response to light and regulate CO2 uptake for photosynthesis and transpiration for uptake of water and nutrients from roots. Light-induced stomatal opening is mediated by activation of the plasma membrane (PM) H+-ATPase in guard cells. Overexpression of PM H+-ATPase in guard cells promotes light-induced stomatal opening, enhancing photosynthesis and growth in Arabidopsis thaliana. In this study, transgenic hybrid aspens overexpressing Arabidopsis PM H+-ATPase (AHA2) in guard cells under the strong guard cell promoter Arabidopsis GC1 (AtGC1) showed enhanced light-induced stomatal opening, photosynthesis, and growth. First, we confirmed that AtGC1 induces GUS expression specifically in guard cells in hybrid aspens. Thus, we produced AtGC1::AHA2 transgenic hybrid aspens and confirmed expression of AHA2 in AtGC1::AHA2 transgenic plants. In addition, AtGC1::AHA2 transgenic plants showed a higher PM H+-ATPase protein level in guard cells. Analysis using a gas exchange system revealed that transpiration and the photosynthetic rate were significantly increased in AtGC1::AHA2 transgenic aspen plants. AtGC1::AHA2 transgenic plants showed a>20% higher stem elongation rate than the wild type (WT). Therefore, overexpression of PM H+-ATPase in guard cells promotes the growth of perennial woody plants.

Identification of Two Arabidopsis thaliana Plasma Membrane Transporters Able to Transport Glucose 1-Phosphate

2019 ◽  
Vol 61 (2) ◽  
pp. 381-392
Author(s):  
Irina Malinova ◽  
Stella Kössler ◽  
Tom Orawetz ◽  
Ulrike Matthes ◽  
Slawomir Orzechowski ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Plasma Membrane ◽  
Carbohydrate Metabolism ◽  
Transport Processes ◽  
Membrane Transporters ◽  
In Planta ◽  
Sugar Derivative ◽  
Sugar Metabolites

Abstract Primary carbohydrate metabolism in plants includes several sugar and sugar-derivative transport processes. Over recent years, evidences have shown that in starch-related transport processes, in addition to glucose 6-phosphate, maltose, glucose and triose-phosphates, glucose 1-phosphate also plays a role and thereby increases the possible fluxes of sugar metabolites in planta. In this study, we report the characterization of two highly similar transporters, At1g34020 and At4g09810, in Arabidopsis thaliana, which allow the import of glucose 1-phosphate through the plasma membrane. Both transporters were expressed in yeast and were biochemically analyzed to reveal an antiport of glucose 1-phosphate/phosphate. Furthermore, we showed that the apoplast of Arabidopsis leaves contained glucose 1-phosphate and that the corresponding mutant of these transporters had higher glucose 1-phosphate amounts in the apoplast and alterations in starch and starch-related metabolism.

scholarly journals Distinct pH regulation of slow and rapid anion channels at the plasma membrane of Arabidopsis thaliana hypocotyl cells

2005 ◽  
Vol 56 (417) ◽  
pp. 1897-1903 ◽  
Author(s):  
Jean Colcombet ◽  
Françoise Lelièvre ◽  
Sébastien Thomine ◽  
Hélène Barbier-Brygoo ◽  
Jean-Marie Frachisse
Keyword(s):  
Arabidopsis Thaliana ◽  
Plasma Membrane ◽  
Ph Regulation ◽  
Anion Channels
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