Dehalococcoides mccartyiStrain JNA Dechlorinates Multiple Chlorinated Phenols Including Pentachlorophenol and Harbors at Least 19 Reductive Dehalogenase Homologous Genes

2014 ◽  
Vol 48 (24) ◽  
pp. 14300-14308 ◽  
Author(s):  
Ashwana D. Fricker ◽  
Sarah L. LaRoe ◽  
Michael E. Shea ◽  
Donna L. Bedard
Keyword(s):  
Chlorinated Phenols ◽  
Reductive Dehalogenase ◽  
Homologous Genes

scholarly journals High frequency of phylogenetically diverse reductive dehalogenase-homologous genes in deep subseafloor sedimentary metagenomes

2014 ◽  
Vol 5 ◽  
Author(s):  
Mikihiko Kawai ◽  
Taiki Futagami ◽  
Atsushi Toyoda ◽  
Yoshihiro Takaki ◽  
Shinro Nishi ◽  
...  
Keyword(s):  
High Frequency ◽  
Reductive Dehalogenase ◽  
Homologous Genes ◽  
Deep Subseafloor

scholarly journals Dehalogenation Activities and Distribution of Reductive Dehalogenase Homologous Genes in Marine Subsurface Sediments

2009 ◽  
Vol 75 (21) ◽  
pp. 6905-6909 ◽  
Author(s):  
Taiki Futagami ◽  
Yuki Morono ◽  
Takeshi Terada ◽  
Anna H. Kaksonen ◽  
Fumio Inagaki
Keyword(s):  
Anaerobic Respiration ◽  
Northwest Pacific ◽  
Diverse Range ◽  
Reductive Dehalogenase ◽  
Homologous Genes ◽  
Eastern Equatorial Pacific ◽  
Sediment Slurry ◽  
Subsurface Sediments ◽  
Juan De Fuca ◽  
Ridge Flank

ABSTRACT Halogenated organic compounds serve as terminal electron acceptors for anaerobic respiration in a diverse range of microorganisms. Here, we report on the widespread distribution and diversity of reductive dehalogenase homologous (rdhA) genes in marine subsurface sediments. A total of 32 putative rdhA phylotypes were detected in sediments from the southeast Pacific off Peru, the eastern equatorial Pacific, the Juan de Fuca Ridge flank off Oregon, and the northwest Pacific off Japan, collected at a maximum depth of 358 m below the seafloor. In addition, significant dehalogenation activity involving 2,4,6-tribromophenol and trichloroethene was observed in sediment slurry from the Nankai Trough Forearc Basin. These results suggest that dehalorespiration is an important energy-yielding pathway in the subseafloor microbial ecosystem.

scholarly journals Identification and Characterization of a Novel CprA Reductive Dehalogenase Specific to Highly Chlorinated Phenols from Desulfitobacterium hafniense Strain PCP-1

2010 ◽  
Vol 76 (22) ◽  
pp. 7536-7540 ◽  
Author(s):  
Ariane Bisaillon ◽  
Réjean Beaudet ◽  
François Lépine ◽  
Eric Déziel ◽  
Richard Villemur
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Mass Spectrometry Analysis ◽  
Activity Levels ◽  
Chlorinated Phenols ◽  
Quinone Oxidoreductase ◽  
Spectrometry Analysis ◽  
Reductive Dehalogenase ◽  
Molecular Masses ◽  
Desulfitobacterium Hafniense

ABSTRACT Desulfitobacterium hafniense strain PCP-1 reductively dechlorinates pentachlorophenol (PCP) to 3-chlorophenol and a variety of halogenated aromatic compounds at the ortho, meta, and para positions. Several reductive dehalogenases (RDases) are thought to be involved in this cascade of dehalogenation. We partially purified a novel RDase involved in the dechlorination of highly chlorinated phenols from strain PCP-1 cultivated in the presence of 2,4,6-trichlorophenol. The RDase was membrane associated, and the activity was sensitive to oxygen, with a half-life of 128 min upon exposure to air. The pH and temperature optima were 7.0 and 55°C, respectively. Several highly chlorinated phenols were dechlorinated at the ortho positions. The highest dechlorinating activity levels were observed with PCP, 2,3,4,5-tetrachlorophenol, and 2,3,4-trichlorophenol. 3-Chloro-4-hydroxyphenylacetate, 3-chloro-4-hydroxybenzoate, dichlorophenols, and monochlorophenols were not dechlorinated. The apparent Km value for PCP was 46.7 μM at a methyl viologen concentration of 2 mM. A mixture of iodopropane and titanium citrate caused a light-reversible inhibition of the dechlorinating activity, suggesting the involvement of a corrinoid cofactor. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the partially purified preparation revealed 2 bands with apparent molecular masses of 42 and 47 kDa. Mass spectrometry analysis using Mascot to search the genome sequence of D. hafniense strain DCB-2 identified the 42-kDa band as NADH-quinone oxidoreductase, subunit D, and the 47-kDa band as the putative chlorophenol RDase CprA3. This is the first report of an RDase with high affinity and high dechlorinating activity toward PCP.

scholarly journals Multiple Reductive-Dehalogenase-Homologous Genes Are Simultaneously Transcribed during Dechlorination by Dehalococcoides-Containing Cultures

2005 ◽  
Vol 71 (12) ◽  
pp. 8257-8264 ◽  
Author(s):  
Alison S. Waller ◽  
Rosa Krajmalnik-Brown ◽  
Frank E. Löffler ◽  
Elizabeth A. Edwards
Keyword(s):  
Vinyl Chloride ◽  
Electron Acceptor ◽  
Reductive Dechlorination ◽  
Amino Acid Identity ◽  
Degenerate Primers ◽  
Acid Identity ◽  
Reductive Dehalogenase ◽  
Homologous Genes ◽  
Differential Transcription ◽  
Transcribed Sequences

ABSTRACT Degenerate primers were used to amplify 14 distinct reductive-dehalogenase-homologous (RDH) genes from the Dehalococcoides-containing mixed culture KB1. Most of the corresponding predicted proteins were highly similar (97 to >99% amino acid identity) to previously reported Dehalococcoides reductive dehalogenases. To examine the differential transcription of these RDH genes, KB1 was split into five subcultures amended with either trichloroethene, cis-1,2-dichloroethene, vinyl chloride, 1,2-dichlorethane, or no chlorinated electron acceptor. Total RNA was extracted following the onset of reductive dechlorination, and RDH transcripts were reverse transcribed and amplified using degenerate primers. The results indicate that the transcription of RDH genes requires the presence of a chlorinated electron acceptor, and for all treatments, multiple RDH genes were simultaneously transcribed, with transcripts of two of the genes being present under all four electron-accepting conditions. Two of the transcribed sequences were highly similar to reported vinyl chloride reductase genes, namely, vcrA from Dehalococcoides sp. strain VS and bvcA from Dehalococcoides sp. strain BAV1. These findings suggest that multiple RDH genes are induced by a single chlorinated substrate and that multiple reductive dehalogenases contribute to chloroethene degradation in KB1.

scholarly journals Distribution of Dehalococcoidia in the Anaerobic Deep Water of a Remote Meromictic Crater Lake and Detection of Dehalococcoidia-Derived Reductive Dehalogenase Homologous Genes

PLoS ONE ◽  
2016 ◽  
Vol 11 (1) ◽  
pp. e0145558 ◽  
Author(s):  
Corinne Biderre-Petit ◽  
Eric Dugat-Bony ◽  
Mickaël Mege ◽  
Nicolas Parisot ◽  
Lorenz Adrian ◽  
...  
Keyword(s):  
Deep Water ◽  
Crater Lake ◽  
Reductive Dehalogenase ◽  
Homologous Genes

Effect of Stresses on Leaf Cuticular Wax Accumulation and Its Relationship to Expression of OsGL1-Homologous Genes in Rice

2013 ◽  
Vol 38 (6) ◽  
pp. 1115-1120
Author(s):  
Ling-Yan ZHOU ◽  
Da-Gang JIANG ◽  
Jing LI ◽  
Wei-Wei CAO ◽  
Chu-Xiong ZHUANG
Keyword(s):  
Cuticular Wax ◽  
Homologous Genes

Activated Sludge Treatment of Kraft Mill Effluents from Conventional and Oxygen Bleaching

1991 ◽  
Vol 24 (3-4) ◽  
pp. 427-430 ◽  
Author(s):  
J. Nevalainen ◽  
P.-R. Rantala ◽  
J. Junna ◽  
R. Lammi
Keyword(s):  
Activated Sludge ◽  
Kraft Pulp ◽  
Activated Sludge Process ◽  
Chlorinated Phenols ◽  
Pulp Mills ◽  
Main Interest ◽  
Sludge Treatment ◽  
Kraft Pulp Mills ◽  
Activated Sludge Processes ◽  
Oxygen Bleaching

Conventional and oxygen bleaching effluents from hardwood kraft pulp mills were treated in laboratory-scale activated sludge processes. The main interest was the fate of organochlorine compounds in the activated sludge process. In the treatment of conventional bleaching wastewaters the BOD7-reduction was 80-91 % and in oxygen bleaching wastewaters 86-93 %. The respective CODCr removals were about 40 % and about 50 %. The AOX reductions were on average 22 % and 40 % in the treatment of conventional and oxygen bleaching effluents, respectively. The reductions of chlorinated phenols, guajacols and catecols were usually more than 50 % in both reactors. Very little accumulation of AOX into the sludge was observed. The stripping of AOX from aeration unit was insignificant.

The Efficiency of a Biological Activated Sludge Effluent Treatment Plant with Extended Aeration

1988 ◽  
Vol 20 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Rurik Skogman ◽  
Reino Lammi
Keyword(s):  
Activated Sludge ◽  
Suspended Solids ◽  
Treatment Plant ◽  
Forest Products ◽  
Effluent Treatment ◽  
Chlorinated Phenols ◽  
Forest Products Industry ◽  
Effluent Treatment Plant ◽  
Closed Systems ◽  
Treating Effluent

The requirements imposed on the Finnish forest products industry by the water authorities have focused on the reduction of BOD and suspended solids in the wastewaters. The industry has tried to comply with these requirements, first through internal measures such as process changes and closed systems. When these have not been sufficient, external treatment has been resorted to. The Wilh. Schauman Company in Jakobstad has chosen activated sludge with extended aeration from among the available methods for treating effluent. The plant has operated since the beginning of 1986 with extremely good results. In addition to the reduction of BOD and suspended solids, there has been a marked decrease of chlorinated phenols. Chlorinated substances with higher molecular weight are also removed during the process.

Sign in / Sign up

Export Citation Format

Share Document