Environmental Gestagens Activate Fathead Minnow (Pimephales promelas) Nuclear Progesterone and Androgen Receptors in Vitro

2014 ◽  
Vol 48 (14) ◽  
pp. 8179-8187 ◽  
Author(s):  
Laura E. Ellestad ◽  
Mary Cardon ◽  
Ian G. Chambers ◽  
Jennifer L. Farmer ◽  
Phillip Hartig ◽  
...  
Keyword(s):  
Fathead Minnow ◽  
Androgen Receptors ◽  
Pimephales Promelas

In vitro-in vivo and cross-life stage extrapolation of uptake and biotransformation of benzo[a]pyrene in the fathead minnow (Pimephales promelas)

2020 ◽  
Vol 228 ◽  
pp. 105616
Author(s):  
Chelsea Grimard ◽  
Annika Mangold-Döring ◽  
Markus Schmitz ◽  
Hattan Alharbi ◽  
Paul D. Jones ◽  
...  
Keyword(s):  
Fathead Minnow ◽  
Life Stage ◽  
Pimephales Promelas

Differential Sensitivity to In Vitro Inhibition of Cytochrome P450 Aromatase (CYP19) Activity Among 18 Freshwater Fishes

2019 ◽  
Vol 170 (2) ◽  
pp. 394-403 ◽  
Author(s):  
Jon A Doering ◽  
Daniel L Villeneuve ◽  
Kellie A Fay ◽  
Eric C Randolph ◽  
Kathleen M Jensen ◽  
...  
Keyword(s):  
Aromatase Inhibitors ◽  
Fathead Minnow ◽  
Pimephales Promelas ◽  
Endocrine Disrupting Chemicals ◽  
Differential Sensitivity ◽  
Japanese Medaka ◽  
Laboratory Model ◽  
Native Fishes ◽  
Ecological Risk Assessments

Abstract There is significant concern regarding potential impairment of fish reproduction associated with endocrine disrupting chemicals. Aromatase (CYP19) is a steroidogenic enzyme involved in the conversion of androgens to estrogens. Inhibition of aromatase by chemicals can result in reduced concentrations of estrogens leading to adverse reproductive effects. These effects have been extensively investigated in a small number of laboratory model fishes, such as fathead minnow (Pimephales promelas), Japanese medaka (Oryzias latipes), and zebrafish (Danio rerio). But, differences in sensitivity among species are largely unknown. Therefore, this study took a first step toward understanding potential differences in sensitivity to aromatase inhibitors among fishes. Specifically, a standard in vitro aromatase inhibition assay using subcellular fractions of whole tissue homogenates was used to evaluate the potential sensitivity of 18 phylogenetically diverse species of freshwater fish to the nonsteroidal aromatase inhibitor fadrozole. Sensitivity to fadrozole ranged by more than 52-fold among these species. Five species were further investigated for sensitivity to up to 4 additional nonsteroidal aromatase inhibitors, letrozole, imazalil, prochloraz, and propiconazole. Potencies of each of these chemicals relative to fadrozole ranged by up to 2 orders of magnitude among the 5 species. Fathead minnow, Japanese medaka, and zebrafish were among the least sensitive to all the investigated chemicals; therefore, ecological risks of aromatase inhibitors derived from these species might not be adequately protective of more sensitive native fishes. This information could guide more objective ecological risk assessments of native fishes to chemicals that inhibit aromatase.

Cloning and In Vitro Expression and Characterization of the Androgen Receptor and Isolation of Estrogen Receptor α from the Fathead Minnow (Pimephales promelas)

2004 ◽  
Vol 38 (23) ◽  
pp. 6314-6321 ◽  
Author(s):  
Vickie S. Wilson ◽  
Mary C. Cardon ◽  
Joseph Thornton ◽  
Joseph J. Korte ◽  
Gerald T. Ankley ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Androgen Receptor ◽  
Fathead Minnow ◽  
Pimephales Promelas ◽  
Estrogen Receptor Α ◽  
In Vitro Expression

scholarly journals Evaluation of Short-Term Fish Reproductive Bioassays for Predicting Effects of a Canadian Bleached Kraft Mill Effluent

2010 ◽  
Vol 45 (2) ◽  
pp. 175-186 ◽  
Author(s):  
Michael R. van den Heuvel ◽  
Pierre H. Martel ◽  
Tibor G. Kovacs ◽  
Deborah L. MacLatchy ◽  
Glen J. Van Der Kraak ◽  
...  
Keyword(s):  
Egg Production ◽  
Fathead Minnow ◽  
Gasterosteus Aculeatus ◽  
Response Pattern ◽  
Pimephales Promelas ◽  
National Response ◽  
Reproductive Endocrine ◽  
Kraft Mill Effluent ◽  
Kraft Mill

Abstract Under the Canadian Environmental Effects Monitoring (EEM) program for pulp and paper effluents, the observation of a national response pattern of decreased gonad size and increased fish condition and liver size has triggered a centralized multiagency investigation of cause (IOC) of reproductive impacts in fishes. The purpose of the component of the IOC study presented here is to compare a number of fish bioassays for determining reproductive and reproductive-endocrine effects of a bleached kraft mill effluent. The bleached kraft mill chosen for this study had demonstrated the national response pattern in previous EEM cycles. The bioassays employed to examine reproduction were fathead minnow (Pimephales promelas) 5- and 21-d, mummichog (Fundulus heteroclitus) 25-d, and zebrafish (Danio rerio) 7-d tests, all of which had egg production as the primary reproductive endpoint. Additional bioassays examining reproductive-endocrine endpoints included a 7-d mummichog test, a 7- and a 21-d threespine stickleback (Gasterosteus aculeatus) test, a rainbow trout (Oncorhynchus mykiss) 7-d test, and in vitro sex steroid receptor and plasma protein binding bioassays. The zebrafish and fathead minnow reproductive tests showed significant suppression of egg production at the 100% effluent concentration. Endocrine data derived from the tests showed that this effluent did not impact steroidogenic endpoints at any concentration. Bioassays showed that this effluent i) was capable of eliciting cytochrome P4501A induction at as low as 10% vol/vol effluent, ii) was weakly androgenic at 10% vol/vol, and iii) showed no evidence of in vivo estrogenicity. These results were consistent with in vitro receptor binding assays showing a highly variable level of androgenic equivalents over six months of effluent testing, with little evidence of estrogenic activity. Bioassay results were consistent in that the overall conclusion was that this effluent has only a weak potential to cause reproductive impairment and would likely not do so at environmentally relevant concentrations. Field studies and a fathead minnow lifecycle study conducted concurrently were in agreement with reproductive bioassay results as white sucker exposed in the receiving environment no longer had significantly reduced gonadal development. Overall, this study provided evidence that the laboratory assays evaluated for various reproductive endpoints have potential application for future IOC work.

Responses in a Fathead Minnow (Pimephales promelas) Lifecycle Test and in Wild White Sucker (Catostomus commersoni) Exposed to a Canadian Bleached Kraft Mill Effluent

2010 ◽  
Vol 45 (2) ◽  
pp. 187-200 ◽  
Author(s):  
Joanne L. Parrott ◽  
L. Mark Hewitt ◽  
Tibor G. Kovacs ◽  
Deborah L. MacLatchy ◽  
Pierre H. Martel ◽  
...  
Keyword(s):  
Egg Production ◽  
Fathead Minnow ◽  
Pimephales Promelas ◽  
Fish Reproduction ◽  
White Sucker ◽  
Catostomus Commersoni ◽  
Liver Size ◽  
Paper Mill Effluent ◽  
Kraft Mill Effluent ◽  
Kraft Mill

Abstract To evaluate currently available bioassays for their use in investigating the causes of pulp and paper mill effluent effects on fish reproduction, the responses of wild white sucker (Catostomus commersoni) collected from the receiving environment at the bleached kraft mill at La Tuque, Quebec, were compared with responses of fathead minnow (Pimephales promelas) exposed to effluent in a laboratory lifecycle test. White sucker collected at effluent exposed sites had increased liver size but none of the reproductive effects that had been documented in earlier field studies at this site. Exposure to 1, 3, 10, 30, and 100% bleached kraft mill effluent (BKME) in the lab led to significantly decreased length, but increased weight and liver size in male fathead minnow. Female length was also decreased and liver size was increased at high effluent exposures. Most effluent concentrations (1 to 30%) significantly increased egg production compared with controls. The fathead minnow lifecycle assay mirrored the effects seen in wild fish captured downstream of the BKME discharge. These results will be used to select short-term fish tests for investigating the causes of and solutions to the effects of mill effluents on fish reproduction.

scholarly journals nLethal and sublethal effects of diluted bitumen and conventional oil on fathead minnow (Pimephales promelas) larvae exposed during their early development

2021 ◽  
pp. 105884
Author(s):  
Roxanne Bérubé ◽  
Charles Gauthier ◽  
Thibault Bourdin ◽  
Marilou Bouffard ◽  
Gaëlle Triffault-Bouchet ◽  
...  
Keyword(s):  
Early Development ◽  
Fathead Minnow ◽  
Sublethal Effects ◽  
Pimephales Promelas ◽  
Conventional Oil
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