Effect of Ingested Tungsten Oxide (WOx) Nanofibers on Digestive Gland Tissue of Porcellio scaber (Isopoda, Crustacea): Fourier Transform Infrared (FTIR) Imaging

2013 ◽  
Vol 47 (19) ◽  
pp. 11284-11292 ◽  
Author(s):  
Sara Novak ◽  
Damjana Drobne ◽  
Lisa Vaccari ◽  
Maya Kiskinova ◽  
Paolo Ferraris ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Tungsten Oxide ◽  
Digestive Gland ◽  
Fourier Transform Infrared ◽  
Porcellio Scaber ◽  
Ftir Imaging ◽  
Gland Tissue

Attenuated total reflection Fourier-transform infrared (ATR-FTIR) imaging of tissues and live cells

2016 ◽  
Vol 45 (7) ◽  
pp. 1850-1864 ◽  
Author(s):  
K. L. Andrew Chan ◽  
Sergei G. Kazarian
Keyword(s):  
Fourier Transform ◽  
Spectroscopic Imaging ◽  
Fourier Transform Infrared ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Live Cells ◽  
Ftir Imaging ◽  
Recent Advances

Recent advances and emerging applications of ATR-FTIR spectroscopic imaging for analysis of biomedical samples have been reviewed.

Monitoring the biochemical alterations in hypertension affected salivary gland tissues using Fourier transform infrared hyperspectral imaging

The Analyst ◽  
2017 ◽  
Vol 142 (8) ◽  
pp. 1269-1275 ◽  
Author(s):  
Shaiju S. Nazeer ◽  
Rarinthorn Samrid ◽  
David Perez-Guaita ◽  
Parichat Prachaney ◽  
Kowit Chaisiwamongkol ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Salivary Gland ◽  
Salivary Glands ◽  
Hyperspectral Imaging ◽  
Fourier Transform Infrared ◽  
Hypertensive Rats ◽  
Ftir Imaging ◽  
Biochemical Alterations

FTIR imaging shows biochemical differences between salivary glands from control and hypertensive rats.

scholarly journals Investigation of Biochemical Alterations in Ischemic Stroke Using Fourier Transform Infrared Imaging Spectroscopy—A Preliminary Study

2019 ◽  
Vol 9 (11) ◽  
pp. 293 ◽  
Author(s):  
Rakib ◽  
Ali ◽  
Yousuf ◽  
Afifi ◽  
Bhatt ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Fourier Transform ◽  
Ischemic Stroke ◽  
Brain Plasticity ◽  
Morphological Changes ◽  
Imaging Spectroscopy ◽  
Total Lipid Content ◽  
Fourier Transform Infrared ◽  
Protein Ratio ◽  
Ftir Imaging

Objective: Brain damage, long-term disability and death are the dreadful consequences of ischemic stroke. It causes imbalance in the biochemical constituents that distorts the brain dynamics. Understanding the sub-cellular alterations associated with the stroke will contribute to deeper molecular understanding of brain plasticity and recovery. Current routine approaches examining lipid and protein biochemical changes post stoke can be difficult. Fourier Transform Infrared (FTIR) imaging spectroscopy can play a vital role in detecting these molecular alterations on a sub-cellular level due to its high spatial resolution, accuracy and sensitivity. This study investigates the biochemical and molecular changes in peri-infract zone (PIZ) (contiguous area not completely damaged by stroke) and ipsi-lesional white matter (WM) (right below the stroke and PIZ regions) nine weeks post photothrombotic ischemic stroke in rats. Materials and Methods: FTIR imaging spectroscopy and transmission electron microscopy (TEM) techniques were applied to investigate brain tissue samples while hematoxylin and eosin (H&E) stained images of adjacent sections were prepared for comparison and examination the morphological changes post stroke. Results: TEM results revealed shearing of myelin sheaths and loss of cell membrane, structure and integrity after ischemic stroke. FTIR results showed that ipsi-lesional PIZ and WM experienced reduction in total protein and total lipid content compared to contra-lesional hemisphere. The lipid/protein ratio reduced in PIZ and adjacent WM indicated lipid peroxidation, which results in lipid chain fragmentation and an increase in olefinic content. Protein structural change is observed in PIZ due to the shift from random coli and α-helical structures to β-sheet conformation. Conclusion: FTIR imaging bio-spectroscopy provide novel biochemical information at sub-cellular levels that be difficult to be obtained by routine approaches. The results suggest that successful therapeutic strategy that is based on administration of anti-oxidant therapy, which could reduce and prevent neurotoxicity by scavenging the lipid peroxidation products. This approach will mitigate tissue damage in chronic ischemic period. FTIR imaging bio-spectroscopy can be used as a powerful tool and offer new approach in stroke and neurodegenerative diseases research.

Monitoring of Biochemical Changes through the C6 Gliomas Progression and Invasion by Fourier Transform Infrared (FTIR) Imaging

2009 ◽  
Vol 81 (22) ◽  
pp. 9247-9256 ◽  
Author(s):  
Abdelilah Beljebbar ◽  
Sylvain Dukic ◽  
Nadia Amharref ◽  
Salima Bellefqih ◽  
Michel Manfait
Keyword(s):  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Biochemical Changes ◽  
Ftir Imaging

scholarly journals Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury

2021 ◽  
Vol 11 (7) ◽  
pp. 918
Author(s):  
Fazle Rakib ◽  
Khalid Al-Saad ◽  
Sebnem Garip Ustaoglu ◽  
Ehsan Ullah ◽  
Raghvendra Mall ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Fourier Transform ◽  
Brain Injury ◽  
Rat Brain ◽  
Mild Traumatic Brain Injury ◽  
Amyloid Β ◽  
Fourier Transform Infrared ◽  
Control Group ◽  
Ftir Imaging ◽  
Imaging Results

Traumatic brain injury (TBI) can be defined as a disorder in the function of the brain after a bump, blow, or jolt to the head, or penetrating head injury. Mild traumatic brain injury (mTBI) can cause devastating effects, such as the initiation of long-term neurodegeneration in brain tissue. In the current study, the effects of mTBI were investigated on rat brain regions; cortex (Co) and corpus callosum (CC) after 24 h (subacute trauma) by Fourier transform infrared (FTIR) imaging and immunohistochemistry (IHC). IHC studies showed the formation of amyloid-β (Aβ) plaques in the cortex brain region of mTBI rats. Moreover, staining of myelin basic protein presented the shearing of axons in CC region in the same group of animals. According to FTIR imaging results, total protein and lipid content significantly decreased in both Co and CC regions in mTBI group compared to the control. Due to this significant decrease in both lipid and protein content, remarkable consistency in lipid/protein band ratio in mTBI and control group, was observed. Significant decrease in methyl content and a significant increase in olefinic content were observed in Co and CC regions of mTBI rat brain tissues. Classification amongst distinguishable groups was performed using principal component analysis (PCA) and hierarchical clustering (HCA). This study established the prospective of FTIR imaging for assessing biochemical changes due to mTBI with high sensitivity, precision and high-resolution.

Applications of Fourier Transform Infrared (FTIR) Imaging

2014 ◽  
pp. 1179-1200 ◽  
Author(s):  
Al de Leon ◽  
Brylee Tiu ◽  
Joey Mangadlao ◽  
Katrina Pangilinan ◽  
Pengfei Cao ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Ftir Imaging
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