Effects of Exposure to 17α-Ethynylestradiol during Sexual Differentiation on the Transcriptome of the African Clawed Frog (Xenopus laevis)

2013 ◽  
Vol 47 (9) ◽  
pp. 4822-4828 ◽  
Author(s):  
Amber R. Tompsett ◽  
Steve Wiseman ◽  
Eric Higley ◽  
John P. Giesy ◽  
Markus Hecker
Keyword(s):  
Xenopus Laevis ◽  
Sexual Differentiation ◽  
African Clawed Frog ◽  
Clawed Frog

Effects of 17α-ethynylestradiol on sexual differentiation and development of the African clawed frog (Xenopus laevis)

2012 ◽  
Vol 156 (3-4) ◽  
pp. 202-210 ◽  
Author(s):  
Amber R. Tompsett ◽  
Steve Wiseman ◽  
Eric Higley ◽  
Sara Pryce ◽  
Hong Chang ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Sexual Differentiation ◽  
African Clawed Frog ◽  
Clawed Frog

Androgen-induced plasticity at a “vocal” neuromuscular synapse

1994 ◽  
Vol 52 ◽  
pp. 32-33
Author(s):  
Darcy B. Kelley ◽  
Martha L. Tobias ◽  
Mark Ellisman
Keyword(s):  
Xenopus Laevis ◽  
Motor Neurons ◽  
Sexual Differentiation ◽  
Molecular Basis ◽  
Neuromuscular Synapse ◽  
Sexually Dimorphic ◽  
Intracellular Protein ◽  
Developmental Program ◽  
Androgen Action ◽  
Clawed Frog

Brain and muscle are sexually differentiated tissues in which masculinization is controlled by the secretion of androgens from the testes. Sensitivity to androgen is conferred by the expression of an intracellular protein, the androgen receptor. A central problem of sexual differentiation is thus to understand the cellular and molecular basis of androgen action. We do not understand how hormone occupancy of a receptor translates into an alteration in the developmental program of the target cell. Our studies on sexual differentiation of brain and muscle in Xenopus laevis are designed to explore the molecular basis of androgen induced sexual differentiation by examining how this hormone controls the masculinization of brain and muscle targets.Our approach to this problem has focused on a highly androgen sensitive, sexually dimorphic neuromuscular system: laryngeal muscles and motor neurons of the clawed frog, Xenopus laevis. We have been studying sex differences at a synapse, the laryngeal neuromuscular junction, which mediates sexually dimorphic vocal behavior in Xenopus laevis frogs.

scholarly journals Developmental changes of purinergic control of intestinal motor activity during metamorphosis in the African clawed frog, Xenopus laevis

2007 ◽  
Vol 292 (5) ◽  
pp. R1916-R1925 ◽  
Author(s):  
Monika Sundqvist
Keyword(s):  
Xenopus Laevis ◽  
Motor Activity ◽  
Receptor Antagonist ◽  
Circular Muscle ◽  
Developmental Changes ◽  
Disulfonic Acid ◽  
Developmental Phase ◽  
African Clawed Frog ◽  
Low Concentrations ◽  
Clawed Frog

Little is known about the purinergic regulation of intestinal motor activity in amphibians. Purinergic control of intestinal motility is subject to changes during development in mammals. The aim of this study was to investigate purinergic control of intestinal smooth muscle in the amphibian Xenopus laevis and explore possible changes in this system during the developmental phase of metamorphosis. Effects of purinergic compounds on mean force and contraction frequency in intestinal circular muscle strips from prometamorphic, metamorphic, and juvenile animals were investigated. Before metamorphosis, low concentrations of ATP reduced motor activity, whereas the effects were reversed at higher concentrations. ATP-induced relaxation was not inhibited by the P2-receptor antagonist pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acid (PPADS) but was blocked by the ecto-nucleotidase inhibitor 6- N, N-diethyl-d-β,γ-dibromomethylene ATP ( ARL67256 ), indicating that an ATP-derived metabolite mediated the relaxation response at this stage. Adenosine induced relaxation before, during, and after metamorphosis, which was blocked by the A1-receptor antagonist 1,3-dipropyl-8-cyclopentylxanthine (DPCPX). The stable ATP-analog adenosine 5′-[γ-thio]-triphosphate (ATPγS) and 2-methylthioATP (2-MeSATP) elicited contractions in the circular muscle strips in prometamorphic tadpoles. However, in juvenile froglets, 2-MeSATP caused relaxation, as did ATPγS at low concentrations. The P2Y11/P2X1-receptor antagonist NF157 antagonized the ATPγS-induced relaxation. The P2X-preferring agonist α-β-methyleneadenosine 5′-triphosphate (α-β-MeATP) evoked PPADS-sensitive increases in mean force at all stages investigated. This study demonstrates the existence of an adenosine A1-like receptor mediating relaxation and a P2X-like receptor mediating contraction in the X. laevis gut before, during, and after metamorphosis. Furthermore, the development of a P2Y11-like receptor-mediated relaxation during metamorphosis is shown.

scholarly journals Toxic effects of carbendazim andn-butyl isocyanate, metabolites of the fungicide benomyl, on early development in the African clawed frog,Xenopus laevis

2008 ◽  
Vol 23 (1) ◽  
pp. 131-144 ◽  
Author(s):  
Chun-Sik Yoon ◽  
Jung-Hyo Jin ◽  
Joo-Hung Park ◽  
Chang-Yeol Yeo ◽  
Song-Ja Kim ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Early Development ◽  
Toxic Effects ◽  
African Clawed Frog ◽  
Clawed Frog

scholarly journals First parasitological study of the African clawed frog (Xenopus laevis, Amphibia) in Chile

2017 ◽  
Vol 26 (2) ◽  
pp. 243-247 ◽  
Author(s):  
Cristóbal Castillo ◽  
Gabriel Lobos ◽  
Daniel González-Acuña ◽  
Lucila Moreno ◽  
Cynthya Elizabeth González ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Introduced Species ◽  
Batrachochytrium Dendrobatidis ◽  
First Record ◽  
Central Chile ◽  
African Clawed Frog ◽  
Parasite Richness ◽  
Native Birds ◽  
Clawed Frog ◽  
Parasitological Study

Abstract Introduced species can arrive into new territories with parasites; however, these species are expected to face lower parasite richness than in their original regions. Both introduced hosts and parasites can affect native fauna. Since their release into the wild in Chile following laboratory use, Xenopus laevis Daudin, 1802 has widely spread throughout central Chile. The only pathogen described on the host is the fungus Batrachochytrium dendrobatidis Longcore, Pessier, Nichols, 1999; thus, this is the first parasitological study of this species in Chile. In 10 localities in central Chile, 179 specimens of X. laevis were captured and examined for parasites in the gastrointestinal tube, cavities, lungs, liver, and skin. Only nine specimens of the genus Contracaecum Railliet, Henry, 1912 were found in six specimens of X. laevis from a private dam in La Patagua. It is likely that these parasites originated from species of native birds. This is the first record of Contracaecum sp. in Chilean amphibians.

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