Interactive Enhancements of Ascorbic Acid and Iron in Hydroxyl Radical Generation in Quinone Redox Cycling

2012 ◽  
Vol 46 (18) ◽  
pp. 10302-10309 ◽  
Author(s):  
Yi Li ◽  
Tong Zhu ◽  
Jincai Zhao ◽  
Bingye Xu
Keyword(s):  
Ascorbic Acid ◽  
Hydroxyl Radical ◽  
Redox Cycling ◽  
Radical Generation

Hydroxyl radical generation by redox cycling of some chemotherapeutic antibiotics

1996 ◽  
Vol 56 (1-4) ◽  
pp. 171-176 ◽  
Author(s):  
T. Michalska ◽  
I. Kruk ◽  
K. Lichszteld ◽  
J. Kladny
Keyword(s):  
Hydroxyl Radical ◽  
Redox Cycling ◽  
Radical Generation

Measurement of Ascorbic Acid (Vitamin C) Induced Hydroxyl Radical Generation in Household Drinking Water

2002 ◽  
Vol 36 (12) ◽  
pp. 1271-1276 ◽  
Author(s):  
Klara U.M. Asplund ◽  
Patric J. Jansson ◽  
Christer Lindqvist ◽  
Tommy Nordström
Keyword(s):  
Ascorbic Acid ◽  
Drinking Water ◽  
Vitamin C ◽  
Hydroxyl Radical ◽  
Radical Generation

scholarly journals Research communication copper-1,10-phenanthroline induces internucleosomal DNA fragmentation in HepG2 cells, resulting from direct oxidation by the hydroxyl radical

1996 ◽  
Vol 317 (1) ◽  
pp. 13-16 ◽  
Author(s):  
Shui Ying TSANG ◽  
Shuk Ching TAM ◽  
Ian BREMNER ◽  
Mark J. BURKITT
Keyword(s):  
Ascorbic Acid ◽  
Low Temperature ◽  
Hydroxyl Radical ◽  
Dna Fragmentation ◽  
Hydroxyl Radicals ◽  
Hepg2 Cells ◽  
Redox Cycling ◽  
Endonuclease Activity ◽  
Direct Oxidation ◽  
Direct Attack

In view of the current speculation regarding the possible role of reactive oxygen species (ROS) in apoptosis, both under physiological conditions and in response to chemicals that promote their intracellular formation, the present investigation was undertaken to examine whether DNA fragmentation during oxidative stress results from endonuclease activity (apoptosis) or from direct attack by ROS. We report that the incubation of HepG2 cells (a human-derived hepatoma cell line) with the copper(II) complex of 1,10-phenanthroline, CuII(OP)2, results in internucleosomal DNA fragmentation, which is widely recognized as being a hallmark of apoptosis. DNA fragmentation did not occur at low temperature, but activity was restored by the addition of ascorbic acid. It is proposed that DNA fragmentation results from the direct attack of hydroxyl radicals upon DNA. Hydroxyl radicals are produced from oxygen by the redox-cycling of CuII(OP)2, which is supported by metabolic processes at normal temperature. At low temperature ascorbic acid provides an artificial cellular reducing environment, thereby restoring hydroxyl radical formation. These findings were confirmed by the detection of internucleosomal DNA fragmentation following the exposure of isolated chromatin to a biomimetic CuII(OP)2 redox-cycling system. We conclude that DNA laddering, the widely employed hallmark of apoptosis, is not unique to endonuclease activity and may also result from direct attack upon DNA by the hydroxyl radical.

scholarly journals Hydroxyl Radical Generation by the Combination of Iron and Ascorbic Acid is Greatly Attenuated but Still Significant in Human Gastric Juice.

2002 ◽  
Vol 41 (12) ◽  
pp. 1213-1214 ◽  
Author(s):  
Tomoyo KAGAWA ◽  
Genzou TAKEMURA ◽  
Xinbin Qiu ◽  
Rumi MARUYAMA ◽  
Ningyuan WANG ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Hydroxyl Radical ◽  
Gastric Juice ◽  
Human Gastric Juice ◽  
Radical Generation

Hydroxyl Radical Generation Mechanism During the Redox Cycling Process of 1,4-Naphthoquinone

2012 ◽  
Vol 46 (5) ◽  
pp. 2935-2942 ◽  
Author(s):  
Yu Shang ◽  
Chenyong Chen ◽  
Yi Li ◽  
Jincai, Zhao ◽  
Tong Zhu
Keyword(s):  
Hydroxyl Radical ◽  
Redox Cycling ◽  
Generation Mechanism ◽  
Cycling Process ◽  
Radical Generation
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