Highly Sensitive and Selective Immuno-Capture/Electrochemical Assay of Acetylcholinesterase Activity in Red Blood Cells: A Biomarker of Exposure to Organophosphorus Pesticides and Nerve Agents

2012 ◽  
Vol 46 (3) ◽  
pp. 1828-1833 ◽  
Author(s):  
Aiqiong Chen ◽  
Dan Du ◽  
Yuehe Lin
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Organophosphorus Pesticides ◽  
Acetylcholinesterase Activity ◽  
Nerve Agents ◽  
Electrochemical Assay ◽  
Biomarker Of Exposure ◽  
Highly Sensitive

scholarly journals Detection of Magnetic Nanoparticles and Fe-hemoglobin inside Red Blood Cells by Using a Highly Sensitive Spin Valve Device

2008 ◽  
Vol 13 (1) ◽  
pp. 30-33 ◽  
Author(s):  
Sang-Hyun Park ◽  
Kwang-Sup Soh ◽  
Do-Guwn Hwang ◽  
Jang-Roh Rhee ◽  
Sang-Suk Lee
Keyword(s):  
Magnetic Nanoparticles ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Spin Valve ◽  
Highly Sensitive

scholarly journals Erythrocyte morphological symmetry analysis to detect sublethal trauma in shear flow

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Antony P. McNamee ◽  
Michael J. Simmonds ◽  
Masataka Inoue ◽  
Jarod T. Horobin ◽  
Masaya Hakozaki ◽  
...  
Keyword(s):  
Shear Flow ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Shape Change ◽  
Bimodal Distribution ◽  
Artificial Organs ◽  
Blood Damage ◽  
Cell Radius ◽  
Highly Sensitive ◽  
Elongation Index

AbstractThe viscoelastic properties of red blood cells (RBC) facilitate flexible shape change in response to extrinsic forces. Their viscoelasticity is intrinsically linked to physical properties of the cytosol, cytoskeleton, and membrane—all of which are highly sensitive to supraphysiological shear exposure. Given the need to minimise blood trauma within artificial organs, we observed RBC in supraphysiological shear through direct visualisation to gain understanding of processes leading to blood damage. Using a custom-built counter-rotating shear generator fit to a microscope, healthy red blood cells (RBC) were directly visualised during exposure to different levels of shear (10–60 Pa). To investigate RBC morphology in shear flow, we developed an image analysis method to quantify (a)symmetry of deforming ellipsoidal cells—following RBC identification and centroid detection, cell radius was determined for each angle around the circumference of the cell, and the resultant bimodal distribution (and thus RBC) was symmetrically compared. While traditional indices of RBC deformability (elongation index) remained unaltered in all shear conditions, following ~100 s of exposure to 60 Pa, the frequency of asymmetrical ellipses and RBC fragments/extracellular vesicles significantly increased. These findings indicate RBC structure is sensitive to shear history, where asymmetrical morphology may indicate sublethal blood damage in real-time shear flow.

Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

1978 ◽  
Vol 36 (2) ◽  
pp. 480-481
Author(s):  
Kosuke Ueda ◽  
Hiroto Washida ◽  
Nakazo Watari
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Cortex ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Renal Lithiasis ◽  
Electron Microscopic ◽  
Hemoglobin C ◽  
First Case ◽  
Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

Densitometric Identification of Primitive Erythroblasts After Reaction With Diaminobenzidine

1973 ◽  
Vol 31 ◽  
pp. 328-329
Author(s):  
John A. Trotter
Keyword(s):  
Red Blood Cells ◽  
Analytical Method ◽  
Blood Cells ◽  
Guinea Pigs ◽  
Specific Protein ◽  
Visual Examination ◽  
Hemoglobin Synthesis ◽  
Peroxidatic Activity ◽  
Small Density

Hemoglobin is the specific protein of red blood cells. Those cells in which hemoglobin synthesis is initiated are the earliest cells that can presently be considered to be committed to erythropoiesis. In order to identify such early cells electron microscopically, we have made use of the peroxidatic activity of hemoglobin by reacting the marrow of erythropoietically stimulated guinea pigs with diaminobenzidine (DAB). The reaction product appeared as a diffuse and amorphous electron opacity throughout the cytoplasm of reactive cells. The detection of small density increases of such a diffuse nature required an analytical method more sensitive and reliable than the visual examination of micrographs. A procedure was therefore devised for the evaluation of micrographs (negatives) with a densitometer (Weston Photographic Analyzer).

Sign in / Sign up

Export Citation Format

Share Document