Phylogenetic Diversity and Metabolic Potential of Activated Sludge Microbial Communities in Full-Scale Wastewater Treatment Plants

2011 ◽  
Vol 45 (17) ◽  
pp. 7408-7415 ◽  
Author(s):  
Chao Yang ◽  
Wei Zhang ◽  
Ruihua Liu ◽  
Qiang Li ◽  
Baobin Li ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Microbial Communities ◽  
Phylogenetic Diversity ◽  
Wastewater Treatment Plants ◽  
Full Scale ◽  
Metabolic Potential

scholarly journals The morphology and metabolic potential of the Chloroflexi in full-scale activated sludge wastewater treatment plants

2018 ◽  
Vol 95 (2) ◽  
Author(s):  
Marta Nierychlo ◽  
Aleksandra Miłobędzka ◽  
Francesca Petriglieri ◽  
Bianca McIlroy ◽  
Per Halkjær Nielsen ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
Full Scale ◽  
Metabolic Potential

An investigation into studying of the activated sludge foaming potential by using physicochemical parameters

2002 ◽  
Vol 46 (1-2) ◽  
pp. 525-528 ◽  
Author(s):  
K. Hladikova ◽  
I. Ruzickova ◽  
P. Klucova ◽  
J. Wanner
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Physicochemical Parameters ◽  
Wastewater Treatment Plants ◽  
Physicochemical Characteristics ◽  
Full Scale ◽  
Aeration Tank ◽  
Foam Formation ◽  
Individual Parameter

This paper examines how the physicochemical characteristics of the solids are related to foam formation and describes how the foaming potential of full-scale plants can be assessed. The relations among activated sludge and biological foam hydrophobicity, scum index, aeration tank cover and filamentous population are evaluated. Individual parameter comparison reveals the scumming intensity can be estimated only on the assumption that foams is already established. None of the above mentioned characteristics can be reliably used to predict the foaming episodes at wastewater treatment plants.

scholarly journals Niche Differentiation among Three Closely RelatedCompetibacteraceaeClades at a Full-Scale Activated Sludge Wastewater Treatment Plant and Putative Linkages to Process Performance

2018 ◽  
Vol 85 (5) ◽  
Author(s):  
Veronica R. Brand ◽  
Laurel D. Crosby ◽  
Craig S. Criddle
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Niche Differentiation ◽  
Full Scale ◽  
Pure Oxygen ◽  
Operational Parameters ◽  
Metabolic Potential ◽  
Operational Conditions

ABSTRACTMultiple clades within a microbial taxon often coexist within natural and engineered environments. Because closely related clades have similar metabolic potential, it is unclear how diversity is sustained and what factors drive niche differentiation. In this study, we retrieved three near-complete Competibacter lineage genomes from activated sludge metagenomes at a full-scale pure oxygen activated sludge wastewater treatment plant. The three genomes represent unique taxa within theCompetibacteraceae. A comparison of the genomes revealed differences in capacity for exopolysaccharide (EPS) biosynthesis, glucose fermentation to lactate, and motility. Using quantitative PCR (qPCR), we monitored these clades over a 2-year period. The clade possessing genes for motility and lacking genes for EPS biosynthesis (CPB_P15) was dominant during periods of suspended solids in the effluent. Further analysis of operational parameters indicate that the dominance of the CPB_P15 clade is associated with low-return activated sludge recycle rates and low wasting rates, conditions that maintain relatively high levels of biomass within the system.IMPORTANCEMembers of the Competibacter lineage are relevant in biotechnology as glycogen-accumulating organisms (GAOs). Here, we document the presence of threeCompetibacteraceaeclades in a full-scale activated sludge wastewater treatment plant and their linkage to specific operational conditions. We find evidence for niche differentiation among the three clades with temporal variability in clade dominance that correlates with operational changes at the treatment plant. Specifically, we observe episodic dominance of a likely motile clade during periods of elevated effluent turbidity, as well as episodic dominance of closely related nonmotile clades that likely enhance floc formation during periods of low effluent turbidity.

scholarly journals The Microbial Database for Danish wastewater treatment plants with nutrient removal (MiDas-DK) – a tool for understanding activated sludge population dynamics and community stability

2013 ◽  
Vol 67 (11) ◽  
pp. 2519-2526 ◽  
Author(s):  
A. T. Mielczarek ◽  
A. M. Saunders ◽  
P. Larsen ◽  
M. Albertsen ◽  
M. Stevenson ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Nutrient Removal ◽  
Wastewater Treatment Plants ◽  
Amplicon Sequencing ◽  
Temporal Variations ◽  
Full Scale ◽  
16S Rrna Amplicon Sequencing ◽  
Specific Factors ◽  
Operational Data

Since 2006 more than 50 Danish full-scale wastewater treatment plants with nutrient removal have been investigated in a project called ‘The Microbial Database for Danish Activated Sludge Wastewater Treatment Plants with Nutrient Removal (MiDas-DK)’. Comprehensive sets of samples have been collected, analyzed and associated with extensive operational data from the plants. The community composition was analyzed by quantitative fluorescence in situ hybridization (FISH) supported by 16S rRNA amplicon sequencing and deep metagenomics. MiDas-DK has been a powerful tool to study the complex activated sludge ecosystems, and, besides many scientific articles on fundamental issues on mixed communities encompassing nitrifiers, denitrifiers, bacteria involved in P-removal, hydrolysis, fermentation, and foaming, the project has provided results that can be used to optimize the operation of full-scale plants and carry out trouble-shooting. A core microbial community has been defined comprising the majority of microorganisms present in the plants. Time series have been established, providing an overview of temporal variations in the different plants. Interestingly, although most microorganisms were present in all plants, there seemed to be plant-specific factors that controlled the population composition thereby keeping it unique in each plant over time. Statistical analyses of FISH and operational data revealed some correlations, but less than expected. MiDas-DK (www.midasdk.dk) will continue over the next years and we hope the approach can inspire others to make similar projects in other parts of the world to get a more comprehensive understanding of microbial communities in wastewater engineering.

scholarly journals Quantification of biologically and chemically bound phosphorus in activated sludge from full-scale plants with biological P-removal

2021 ◽  
Author(s):  
Francesca Petriglieri ◽  
Jette F. Petersen ◽  
Miriam Peces ◽  
Marta Nierychlo ◽  
Kamilla Hansen ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Mass Balance ◽  
Wastewater Treatment Plants ◽  
Raman Microspectroscopy ◽  
Full Scale ◽  
P Fractions ◽  
P Content ◽  
Total P ◽  
P Removal

AbstractLarge amounts of phosphorus (P) are present in activated sludge from municipal wastewater treatment plants, where it exists in the form of metal salt precipitates or biologically bound into the biomass as nucleic acids, cell membrane components, and the extracellular polymeric substances or, in special polyphosphate-accumulating organisms (PAOs), as intracellular polyphosphate. Only recently, methods that reliably allow an absolute quantification of the different P-fractions, such as sequential extraction, Raman microspectroscopy, solid-state 31P magic angle spinning (MAS) NMR, and solution state 31P NMR have been developed. This study combines these techniques to obtain a comprehensive P mass-balance of activated sludge from four wastewater treatment plants with enhanced biological phosphate removal (EBPR). The total content of P and various cations was measured by chemical analysis (ICP-OES), and different P fractions were extracted for chemical characterization. Chemically bound P constituted 38-69% of total P, most likely in the form of Fe, Mg, or Al minerals, while organically bound P constituted 7-9%. By using Raman microspectroscopy and solution state 31P NMR and 31P MAS NMR spectroscopy before and after anaerobic P-release experiments, poly-P was quantified and constituted 22-54% of total P in the activated sludges and was found in approx. 25% of all bacterial cells. Moreover, Raman microspectroscopy in combination with fluorescence in situ hybridization (FISH) was used to quantify the species-specific intracellular poly-P of known PAO genera (Tetrasphaera, Ca. Accumulibacter, Dechloromonas) and other microorganisms known to possess high level of poly-P, such as the filamentous Ca. Microthrix. They were all abundant, as measured by quantitative-FISH and amplicon sequencing, and accumulated large amount of poly-P, depending on their cell-size, contributing substantially to the P-removal. Interestingly, in all four EBPR plants investigated, only 1-13% of total poly-P was stored by unidentified PAO, highlighting that most PAOs in the full-scale EBPR plants investigated are now known.HighlightsExhaustive P mass-balance of main organic and inorganic P-species in four EBPR plantsQuantification of poly-P of FISH-defined PAO and other species with high P contentTotal P content was 36-50 mgP/gSS of which 31-62% was in biomass and as poly-PA high fraction of all cells (25-30%) contained a high content of poly-PKnown PAOs contained almost all poly-P in the EBPR plants investigated

Abundance of amoA genes of ammonia-oxidizing archaea and bacteria in activated sludge of full-scale wastewater treatment plants

2011 ◽  
Vol 102 (4) ◽  
pp. 3694-3701 ◽  
Author(s):  
Tawan Limpiyakorn ◽  
Puntipar Sonthiphand ◽  
Chaiwat Rongsayamanont ◽  
Chongrak Polprasert
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
Full Scale ◽  
Ammonia Oxidizing Archaea

The development and use of real-time PCR for the quantification of nitrifiers in activated sludge

2002 ◽  
Vol 46 (1-2) ◽  
pp. 267-272 ◽  
Author(s):  
S.J. Hall ◽  
P. Hugenholtz ◽  
N. Siyambalapitiya ◽  
J. Keller ◽  
L.L. Blackall
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Real Time ◽  
Real Time Pcr ◽  
Wastewater Treatment Plants ◽  
Quantitative Polymerase Chain Reaction ◽  
Analytical Data ◽  
Full Scale ◽  
Rrna Gene ◽  
Dna Templates

Chemical analytical data has long been used to monitor the performance of activated sludge plants even though the process relies on the performance of microorganisms. It is now evident that a rapid and reliable quantitative method is required, to be able to monitor the organisms responsible for nutrient transformation and their activities, allowing avenues for more efficient nutrient removal. The development of real-time or quantitative polymerase chain reaction (PCR) also known as TaqMan® or 5′-nuclease assay has allowed the rapid, quantitative analysis of DNA templates, eliminating some of the variability traditionally associated with other quantitative techniques. In this study analysis of Nitrospira spp., one of the key organisms in nitrite oxidation in wastewater treatment, was used to validate real-time PCR for the their quantification in activated sludge. A probe and primer set, targeting the 16S rRNA gene of Nitrospira spp. was designed according to the constraints of the TaqMan® specifications. Samples used to evaluate the method included DNA from the sludge from full-scale wastewater treatment plants and laboratory scale systems. The reproducibility, quantitative efficiency and specificity were assessed in the evaluation. It was concluded that the method is sensitive and reproducible but has some constraints on the quantitative efficiency. A survey of full-scale systems for Nitrospira spp. was carried out and the results are presented here.

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