Mechanisms of Humic Acids Degradation by White Rot Fungi Explored Using1H NMR Spectroscopy and FTICR Mass Spectrometry

2011 ◽  
Vol 45 (7) ◽  
pp. 2748-2754 ◽  
Author(s):  
Tzafrir Grinhut ◽  
Norbert Hertkorn ◽  
Philippe Schmitt-Kopplin ◽  
Yitzhak Hadar ◽  
Yona Chen
Keyword(s):  
Mass Spectrometry ◽  
Nmr Spectroscopy ◽  
Humic Acids ◽  
White Rot Fungi ◽  
White Rot ◽  
Fticr Mass Spectrometry

Biodegradation and Biotransformation of Lindane by DDT-Degrading White Rot Fungi

2013 ◽  
Vol 726-731 ◽  
pp. 39-42
Author(s):  
Peng Fei Xiao
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Pure Culture ◽  
White Rot Fungi ◽  
White Rot ◽  
Gas Chromatography Mass Spectrometry ◽  
First Report ◽  
Chromatography Mass Spectrometry ◽  
Metabolic Products ◽  
Phlebia Brevispora

Lindane (γ-hexachlorocyclohexane) was used as the substrate for a degradation experiment with the white rot fungiPhlebia brevisporaTMIC34596 andPhlebia lindtneriGB1027, which are capable of degrading DDT. Pure culture of both fungi showed that about 40% of lindane was degraded after 7 days of incubation, while over 70% of lindane was degraded after 28 days of incubation. Eight metabolic products such as pentachlorocyclohexanol, dihydroxytetrachlorocyclohexane and trihydroxytrichlorocyclohexane were detected from both fungal cultures using gas chromatography/mass spectrometry (GC/MS). This is the first report of the biodegradation of lindane through successive Cl/OH substitution pathway by microorganisms.

Identification of metabolites produced by Phanerochaete chrysosporium in the presence of amlodipine orotate using metabolomics

2015 ◽  
Vol 72 (7) ◽  
pp. 1140-1146 ◽  
Author(s):  
R. B. De Sotto ◽  
K. I. Kim ◽  
S. Kim ◽  
K. G. Song ◽  
Y. Park
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Phanerochaete Chrysosporium ◽  
White Rot Fungi ◽  
Degradation Pathway ◽  
White Rot ◽  
Original Form ◽  
P Value ◽  
Significance Threshold ◽  
Fungal Biodegradation

Pharmaceuticals are very useful in treating human diseases but they are excreted to the environment sometimes in their original form or as byproducts of human metabolism. Pharmaceuticals and their metabolites have been proven by studies to be harmful to non-target ecological species and may be persistent in different water matrices. In this regard, there is an emergent need to eliminate these compounds to prevent their adverse effects on aquatic species. Biodegradation using white-rot fungi is a promising technology for the removal of recalcitrant compounds; however, products of fungal biodegradation can also be detrimental. In this novel study, we evaluated the ability of Phanerochaete chrysosporium to degrade amlodipine, an anti-hypertensive drug which was recently found in water systems. Analysis of amlodipine metabolites was done using quadrupole time-of-flight liquid chromatography mass spectrometry after the degradation set-up of 120 hours. Pharmaceutical degradation was seen using triple quadrupole liquid chromatography tandem mass spectrometry. Ninety-two significant metabolites (P-value ≤ 0.05) were significantly expressed after false discovery rate adjustment at a significance threshold of q = 0.05. Pyridine derivatives which were identified from samples became the basis of the proposed degradation pathway of amlodipine in this study.

scholarly journals Enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Tangwu Cui ◽  
Bo Yuan ◽  
Haiwei Guo ◽  
Hua Tian ◽  
Weimin Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Enzymatic Activity ◽  
Quantum Coherence ◽  
Lignin Degradation ◽  
Synergistic Effects ◽  
White Rot Fungi ◽  
White Rot ◽  
Lignin Biodegradation ◽  
Short Period ◽  
Product Mapping

Abstract Background As one of the major components of lignocellulosic biomass, lignin has been considered as the most abundant renewable aromatic feedstock in the world. Comparing with thermal or catalytic strategies for lignin degradation, biological conversion is a promising approach featuring with mild conditions and diversity, and has received great attention nowadays. Results In this study, a consortium of white rot fungi composed of Lenzites betulina and Trametes versicolor was employed to enhance the ligninolytic enzyme activity of laccase (Lac) and manganese peroxidase (MnP) under microbial synergism. The maximum enzymatic activity of Lac and MnP was individually 18.06 U mL−1 and 13.58 U mL−1 along with a lignin degradation rate of 50% (wt/wt), which were achieved from batch cultivation of the consortium. The activities of Lac and MnP obtained from the consortium were both improved more than 40%, as compared with monocultures of L. betulina or T. versicolor under the same culture condition. The enhanced biodegradation performance was in accordance with the results observed from scanning electron microscope (SEM) of lignin samples before and after biodegradation, and secondary-ion mass spectrometry (SIMS). Finally, the analysis of heteronuclear single quantum coherence (HSQC) NMR and gas chromatography–mass spectrometry (GC–MS) provided a comprehensive product mapping of the lignin biodegradation, suggesting that the lignin has undergone depolymerization of the macromolecules, side-chain cleavage, and aromatic ring-opening reactions. Conclusions Our results revealed a considerable escalation on the enzymatic activity obtained in a short period from the cultivation of the L. betulina or T. versicolor due to the enhanced microbial synergistic effects, providing a potential bioconversion route for lignin utilization.

scholarly journals Mechanism of lignin degradation via white rot fungi explored using spectral analysis and gas chromatography-mass spectrometry

BioResources ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. 5494-5507
Author(s):  
Libo Jin ◽  
Guoming Zeng ◽  
Haojie Chen ◽  
Lei Wang ◽  
Hao Ji ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Enzyme Activity ◽  
Lignin Degradation ◽  
White Rot Fungi ◽  
White Rot ◽  
Gas Chromatography Mass Spectrometry ◽  
Dependent Manner ◽  
Alkali Lignin ◽  
Chromatography Mass Spectrometry

The mechanism of lignin degradation via white rot fungi was studied. Phanerochaete chrysosporium and Pleurotus ostreatus were used for all the experiments, i.e., measuring the concentration and structure of alkali lignins and studying the effect of the substrate concentration and enzyme activity on the removal. Gas chromatography-mass spectrometry was performed on the reaction liquid of the lignin degradation enzyme system. Alkali lignin had a characteristic absorption spectrum with a peak at approximately 280 nm. Precipitation in the laccase (Lac) degradation system occurred earlier, as well as being more obvious than that in the manganese peroxidase (Mnp) degradation system. The maximum removal was 29.4% in the Mnp degradation system at a concentration of 40 mg/L. The removal increased in a concentration-dependent manner in the Lac degradation system. The increase in Mnp and Lac enzyme activity led to an increased alkali lignin removal. The removal of the control group was significantly lower than the experimental degradation systems. The degradation mainly produced organic acids, esters, and aromatic substances. In conclusion, white rot fungi could effectively remove alkali lignin, in which precipitation played a major role, followed by enzymolysis; the enzymolysis was associated with the alkali lignin concentration and enzyme activity.

Characterization of Bio-oil from Hydrothermal Liquefaction of Organic Waste by NMR Spectroscopy and FTICR Mass Spectrometry

ChemSusChem ◽  
2012 ◽  
Vol 6 (1) ◽  
pp. 160-167 ◽  
Author(s):  
Irene Leonardis ◽  
Stefano Chiaberge ◽  
Tiziana Fiorani ◽  
Silvia Spera ◽  
Ezio Battistel ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Nmr Spectroscopy ◽  
Organic Waste ◽  
Hydrothermal Liquefaction ◽  
Fticr Mass Spectrometry ◽  
Bio Oil

scholarly journals Enhanced Lignin Biodegradation by Consortium of White rot Fungi: Microbial Synergistic Effects and Product Mapping

2021 ◽  
Author(s):  
Tangwu Cui ◽  
Bo Yuan ◽  
Haiwei Guo ◽  
Hua Tian ◽  
Weimin Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantum Coherence ◽  
Synergistic Effects ◽  
White Rot Fungi ◽  
White Rot ◽  
Gas Chromatography Mass Spectrometry ◽  
Lignin Biodegradation ◽  
Biological Degradation ◽  
Short Period ◽  
Product Mapping

Abstract Background : As one of the major components in lignocellulosic biomass, lignin has been considered as the most abundant renewable aromatic feedstock in the world. Featuring with mild conditions and diversity, biological degradation of lignin is a promising approach comparing with thermal or catalytic ones. Results : In this study, a consortium of white rot fungi composed of Lenzites betulina and Trametes versicolor was employed in order to enhance the ligninolytic enzyme activity of laccase (Lac) and manganese peroxidase (MnP) under microbial synergism. The maximum enzymatic activity of Lac and MnP was individually 18.06 U·mL-1 and 13.58 U·mL-1 along with a lignin degradation rate of 50%, which were achieved from batch cultivation of the consortium. The activity of Lac and MnP obtained from the consortium was all improved more than 40%, compared with monocultures of L. betulina or T. versicolor under the same culture condition. Our findings of enhanced biodegradation were in accordance with the results observed from scanning electron microscope (SEM) and secondary-ion mass spectrometry (SIMS). Finally, the analysis of heteronuclear single quantum coherence (HSQC) NMR and gas chromatography-mass spectrometry (GC-MS) provided a comprehensive product mapping of the lignin biodegradation, suggesting that the lignin has undergone depolymerization of the macromolecules, side-chain cleavage, and aromatic ring-opening reactions. Conclusions : Our results revealed a considerable escalation on the enzymatic activities obtained in a short period from the cultivation of the L. betulina or T. versicolor due to the enhanced microbial synergistic effects, providing a potential bioconversion route for the applications of lignin utilization.

OPTIMIZATION OF BIOMASS AND ENDO-B-1,4-GLUCANASE BY WHITE ROT FUNGI NATIVE FROM ARGENTINA

2017 ◽  
Vol 16 (11) ◽  
pp. 2581-2588
Author(s):  
Ernesto M. Giorgio ◽  
Maria I. Fonseca ◽  
Andrea L. Morales ◽  
Pedro D. Zapata ◽  
Laura L. Villalba
Keyword(s):  
White Rot Fungi ◽  
White Rot
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