Mechanistic Toxicity Assessment of Nanomaterials by Whole-Cell-Array Stress Genes Expression Analysis

2010 ◽  
Vol 44 (15) ◽  
pp. 5964-5970 ◽  
Author(s):  
Na Gou ◽  
Annalisa Onnis-Hayden ◽  
April Z. Gu
Keyword(s):  
Expression Analysis ◽  
Toxicity Assessment ◽  
Cell Array ◽  
Whole Cell ◽  
Stress Genes ◽  
Genes Expression

scholarly journals ADR1-Mediated Transcriptional Activation Requires the Presence of an Intact TFIID Complex

1998 ◽  
Vol 18 (10) ◽  
pp. 5861-5867 ◽  
Author(s):  
Philip B. Komarnitsky ◽  
Edward R. Klebanow ◽  
P. Anthony Weil ◽  
Clyde L. Denis
Keyword(s):  
Transcriptional Activation ◽  
Yeast Cells ◽  
Binding Studies ◽  
Whole Cell ◽  
Transactivation Domains ◽  
Cell Extracts ◽  
Genes Expression ◽  
Tfiid Complex

ABSTRACT The yeast transcriptional activator ADR1, which is required forADH2 and other genes’ expression, contains four transactivation domains (TADs). While previous studies have shown that these TADs act through GCN5 and ADA2, and presumably TFIIB, other factors are likely to be involved in ADR1 function. In this study, we addressed the question of whether TFIID is also required for ADR1 action. In vitro binding studies indicated that TADI of ADR1 was able to retain TAFII90 from yeast extracts and TADII could retain TBP and TAFII130/145. TADIV, however, was capable of retaining multiple TAFIIs, suggesting that TADIV was binding TFIID from yeast whole-cell extracts. The ability of TADIV truncation derivatives to interact with TFIID correlated with their transcription activation potential in vivo. In addition, the ability of LexA-ADR1-TADIV to activate transcription in vivo was compromised by a mutation in TAFII130/145. ADR1 was found to associate in vivo with TFIID in that immunoprecipitation of either TAFII90 or TBP from yeast whole-cell extracts specifically coimmunoprecipitated ADR1. Most importantly, depletion of TAFII90 from yeast cells dramatically reducedADH2 derepression. These results indicate that ADR1 physically associates with TFIID and that its ability to activate transcription requires an intact TFIID complex.

Survival genes expression analysis following ionizing radiation to LiCl treated KG1a cells

2020 ◽  
Vol 96 (5) ◽  
pp. 671-688
Author(s):  
Yogesh Kumar Verma ◽  
Ajay Kumar Singh ◽  
Gangenahalli Ugraiah Gurudutta
Keyword(s):  
Ionizing Radiation ◽  
Expression Analysis ◽  
Genes Expression

Whole cell immobilised biosensors for toxicity assessment of a wastewater treatment plant treating phenolics-containing waste

2003 ◽  
Vol 487 (1) ◽  
pp. 61-74 ◽  
Author(s):  
Jim C Philp ◽  
Séverine Balmand ◽  
Eva Hajto ◽  
Mark J Bailey ◽  
Siouxsie Wiles ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Toxicity Assessment ◽  
Whole Cell

scholarly journals VEGF-B, VEGF-A, FLT-1, KDR, ERBB2, EGFR, GRB2, RAC1, CDH1 and HYAL-1 Genes Expression Analysis in Canine Mammary Gland Tumors and the Association with Tumor ClinicoPathological Parameters and Dog Breed Assessment

2021 ◽  
Vol 8 (10) ◽  
pp. 212
Author(s):  
Simona Sakalauskaitė ◽  
Violeta Šaltenienė ◽  
Darja Nikitina ◽  
Rasa Ugenskienė ◽  
Vita Riškevičienė ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Expression Analysis ◽  
Clinicopathological Parameters ◽  
German Shepherd ◽  
Mammary Gland Tumors ◽  
Genes Expression ◽  
Mixed Breed ◽  
Size Grade ◽  
Canine Mammary Gland

Canine mammary gland tumors (CMTs) are one of the most prevalent cancers in dogs and a good model for human breast cancer (BC), however gene expression analysis of CMTs is scarce. Although divergence of genes expression has been found in BC of different human races, no such research of different dog’s breeds has been done. The purpose of this study was to investigate expression of the VEGF-B, VEGF-A, FLT-1, KDR, ERBB2, EGFR, GRB2, RAC1, CDH1 and HYAL-1 genes of canine mammary carcinomas, compare the expression levels with clinicopathological parameters and analyze expression disparities between different breeds. Carcinomas and adjacent tissues were collected from female dogs to perform routine histopathology, immunohistochemistry (IHC) and quantitative real-time polymerase chain reaction (qRT-PCR). We found that VEGF-B and EGFR genes were overexpressed in the mammary gland carcinomas compared to adjacent tissue. VEGF-B gene expression had associations with different parameters (tumor size, grade, and absence of metastasis). Furthermore, differences in VEGF-B, FLT1, ERBB2, GRB2, RAC1, CDH1 and HYAL-1 genes expression have been found in different breed dogs (German Shepherd, Yorkshire Terrier) and mixed-breed dogs indicating that a dog’s breed could determine a molecular difference, outcome of cancer and should be accounted as a confounding factor in the future gene expression research.

Comprehensive Genes Expression Analysis in Bovine Trophoblastic Cell Lines.

2011 ◽  
Vol 85 (Suppl_1) ◽  
pp. 451-451
Author(s):  
Kazuyoshi Hashizume ◽  
Daichi Watanabe ◽  
Keiichiro Kizaki ◽  
Yasunori Suzuki ◽  
Misa Hosoe ◽  
...  
Keyword(s):  
Cell Lines ◽  
Expression Analysis ◽  
Trophoblastic Cell ◽  
Genes Expression

Co-exposure to foodborne and waterborne ZnO nanoparticles in aquatic sediment environments enhances DNA damage and stress gene expression in freshwater Asian clam Corbicula fluminea

2020 ◽  
Vol 7 (4) ◽  
pp. 1252-1265
Author(s):  
Chi-Wei Huang ◽  
Chun-Han Chang ◽  
Shang-Wei Li ◽  
Pei-Ling Yen ◽  
Vivian Hsiu-Chuan Liao
Keyword(s):  
Gene Expression ◽  
Dna Damage ◽  
Zno Nanoparticles ◽  
Corbicula Fluminea ◽  
Asian Clam ◽  
Stress Genes ◽  
Genes Expression ◽  
Clam Corbicula ◽  
Aquatic Sediment ◽  
Stress Gene

Co-exposure to foodborne and waterborne ZnO nanoparticles in aquatic sediment environments increases mortality, Zn bioaccumulation, stress genes expression, and DNA damage in freshwater Asian clam Corbicula fluminea.

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