Proteomics for the Analysis of Environmental Stress Responses in Organisms

2007 ◽  
Vol 41 (20) ◽  
pp. 6891-6900 ◽  
Author(s):  
Victor J. Nesatyy ◽  
Marc J.-F. Suter
Keyword(s):  
Environmental Stress ◽  
Stress Responses

scholarly journals Hypusination, a Metabolic Posttranslational Modification of eIF5A in Plants during Development and Environmental Stress Responses

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1261
Author(s):  
Péter Pálfi ◽  
László Bakacsy ◽  
Henrietta Kovács ◽  
Ágnes Szepesi
Keyword(s):  
Amino Acid ◽  
Environmental Stress ◽  
Plant Development ◽  
Stress Responses ◽  
Posttranslational Modification ◽  
Proper Function ◽  
Signal Pathways ◽  
Deoxyhypusine Synthase ◽  
Deoxyhypusine Hydroxylase ◽  
Eukaryotic Translation

Hypusination is a unique posttranslational modification of eIF5A, a eukaryotic translation factor. Hypusine is a rare amino acid synthesized in this process and is mediated by two enzymes, deoxyhypusine synthase (DHS) and deoxyhypusine hydroxylase (DOHH). Despite the essential participation of this conserved eIF5A protein in plant development and stress responses, our knowledge of its proper function is limited. In this review, we demonstrate the main findings regarding how eIF5A and hypusination could contribute to plant-specific responses in growth and stress-related processes. Our aim is to briefly discuss the plant-specific details of hypusination and decipher those signal pathways which can be effectively modified by this process. The diverse functions of eIF5A isoforms are also discussed in this review.

scholarly journals A Drosophila p38 orthologue is required for environmental stress responses

EMBO Reports ◽  
2004 ◽  
Vol 5 (11) ◽  
pp. 1058-1063 ◽  
Author(s):  
Caroline R Craig ◽  
Jill L Fink ◽  
Yoshimasa Yagi ◽  
Y Tony Ip ◽  
Ross L Cagan
Keyword(s):  
Environmental Stress ◽  
Stress Responses

MicroRNAs mediated environmental stress responses and toxicity signs in teleost fish species

Aquaculture ◽  
2021 ◽  
pp. 737310
Author(s):  
Sayed HaidarAbbas Raz ◽  
Sameh A. Abdelnour ◽  
Mashael Alhumaidi Alotaibi ◽  
Qwiat AlGabbani ◽  
Mohammed A.E. Naiel ◽  
...  
Keyword(s):  
Environmental Stress ◽  
Fish Species ◽  
Stress Responses ◽  
Teleost Fish

scholarly journals Environmental Stress Responses of DnaJA1, DnaJB12 and DnaJC8 in Apis cerana cerana

2018 ◽  
Vol 9 ◽  
Author(s):  
Guilin Li ◽  
Hang Zhao ◽  
Xuemei Zhang ◽  
Yanming Zhang ◽  
Huayu Zhao ◽  
...  
Keyword(s):  
Environmental Stress ◽  
Stress Responses ◽  
Apis Cerana ◽  
Apis Cerana Cerana

scholarly journals Longitudinal metatranscriptomic analysis of a meat spoilage microbiome detects abundant continued fermentation and environmental stress responses during shelf life and beyond

2020 ◽  
Author(s):  
Jenni Hultman ◽  
Per Johansson ◽  
Johanna Björkroth
Keyword(s):  
Environmental Stress ◽  
Shelf Life ◽  
Stress Responses ◽  
Pentose Phosphate ◽  
Quality Analysis ◽  
Microbial Succession ◽  
Pronounced Difference ◽  
Meat Spoilage ◽  
Microbiological Methods ◽  
16S Rna

AbstractMicrobial food spoilage is a complex phenomenon associated with the succession of the specific spoilage organisms (SSO) over the course of time. We performed a longitudinal metatranscriptomic study on a modified atmosphere packaged (MAP) beef product to increase understanding of the longitudinal behavior of a spoilage microbiome during shelf life and onward. Based on the annotation of the mRNA reads, we recognized three stages related to the active microbiome that were descriptive for the sensory quality of the beef: acceptable product (AP), early spoilage (ES) and late spoilage (LS). Both the 16S RNA taxonomic assignments from the total RNA and functional annotations of the active genes showed that these stages were significantly different from each other. However, the functional gene annotations showed more pronounced difference than the taxonomy assignments. Psychrotrophic lactic acid bacteria (LAB) formed the core of the SSO according to the transcribed reads. Leuconostoc species were the most abundant active LAB throughout the study period, whereas the activity of Streptococcaceae (mainly Lactococcus) increased after the product was spoiled. In the beginning of the experiment, the community managed environmental stress by cold-shock responses which were followed by the expression of the genes involved in managing oxidative stress. Glycolysis, pentose phosphate pathway and pyruvate metabolism were active throughout the study at a relatively stable level. However, the proportional activity of the enzymes in these pathways changed over time. For example, acetate kinase activity was characteristic for the AP stage whereas formate C-acetyltransferase transcription was associated with spoilage.ImportanceIt is generally known which organisms are the typical SSO in foods, whereas the actively transcribed genes and pathways during microbial succession are poorly understood. This knowledge is important since better approaches to food quality evaluation and shelf life determination are needed. Thus, we conducted this study to find longitudinal markers that are connected to quality deterioration. These kind of RNA markers could be used to develop novel type of rapid quality analysis tools in the future. New tools are needed since even though SSO can be detected and their concentrations determined using the current microbiological methods, results from these analyses cannot predict how close timewise a spoilage community is from production of clear sensory defects. Main reason for this is that the species composition of a spoilage community does not change dramatically during late shelf life, whereas the ongoing metabolic activities lead to the development of notable sensory deterioration.

scholarly journals Environmental stress responses in yeasts and lactic acid bacteria strains isolated from dairy traditional Romanian fermented products

2021 ◽  
Vol 26 (2) ◽  
pp. 2548-2559
Author(s):  
VIORICA CORBU ◽  
◽  
STEFANA PETRUT ◽  
TATIANA VASSU ◽  
DIANA PELINESCU ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Cell Growth ◽  
Lactic Acid Bacteria ◽  
Environmental Stress ◽  
Stress Responses ◽  
Dairy Products ◽  
Growth Parameters ◽  
Great Opportunity ◽  
Fermented Dairy Products ◽  
Fermented Dairy

During last decades, there is a growing interest for characterizing new microbial strains isolated from various sources (plants, soil and natural fermentative processes), in order to enhance industrial productivity. The aim of the present study was to assess the profile of cell growth parameters and biomass accumulation of 15 newly isolated yeast and lactic acid bacteria (LAB) strains from Romanian spontaneous fermented dairy products under different environmental stress conditions (chemical and physical). On this purpose, the yeast and LAB strains were characterized and identified using MALDI-TOF MS and selected for their biotechnological potential. Cell growth was evaluated in presence of extreme pH values, temperatures and different NaCl concentrations. All strains included in this study grew well under their optimal conditions; some of them preferred extreme parameters: acid / very alkaline pH, high temperatures or NaCl concentration The characterization of microbiota from Romanian spontaneous fermented dairy products might represent a great opportunity for the development of dairy industry using native microorganisms, preserving thus the Romanian biodiversity and cultural heritage.

scholarly journals Transcriptional induction of capsidiol synthesis genes by wounding can promote pathogen signal-induced capsidiol synthesis

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Tomoya Kojima ◽  
Nobuhide Asakura ◽  
Shiori Hasegawa ◽  
Taishi Hirasawa ◽  
Yuri Mizuno ◽  
...  
Keyword(s):  
Environmental Stress ◽  
Stress Responses ◽  
Transcript Level ◽  
Pathogen Invasion ◽  
Wound Responses ◽  
Mitogen Activated Protein ◽  
Physical Barriers ◽  
Reporter Gene Analysis ◽  
Almost All ◽  
Artificial Activation

Abstract Background Plants are exposed to various forms of environmental stress. Penetration by pathogens is one of the most serious environmental insults. Wounding caused by tissue damage or herbivory also affects the growth and reproduction of plants. Moreover, wounding disrupts physical barriers present at the plant surface and increases the risk of pathogen invasion. Plants cope with environmental stress by inducing a variety of responses. These stress responses must be tightly controlled, because their unnecessary induction is detrimental to plant growth. In tobacco, WIPK and SIPK, two wound-responsive mitogen-activated protein kinases, have been shown to play important roles in regulating wound responses. However, their contribution to downstream wound responses such as gene expression is not well understood. Results To identify genes regulated by WIPK and SIPK, the transcriptome of wounded WIPK/SIPK-suppressed plants was analyzed. Among the genes down-regulated in WIPK/SIPK-suppressed plants, the largest group consisted of those involved in the production of antimicrobial phytoalexins. Almost all genes involved in the biosynthesis of capsidiol, a major phytoalexin in tobacco, were transcriptionally induced by wounding in WIPK/SIPK-dependent and -independent manners. 5-epi-aristolochene synthase (EAS) is the committing enzyme for capsidiol synthesis, and the promoter of EAS4, a member of the EAS family, was analyzed. Reporter gene analysis revealed that at least two regions each 40–50 bp length were involved in activation of the EAS4 promoter by wounding, as well as by artificial activation of WIPK and SIPK. Unlike transcripts of the capsidiol synthesis genes, accumulation of EAS protein and capsidiol itself were not induced by wounding; however, wounding significantly enhanced their subsequent induction by a pathogen-derived elicitor. Conclusions Our results suggest a so-called priming phenomenon since the induction of EAS by wounding is only visible at the transcript level. By inducing transcripts, not the proteins, of EAS and possibly other capsidiol synthesis genes at wound sites, plants can produce large quantities of capsidiol quickly if pathogens invade the wound site, whereas plants can minimize energy loss and avoid the cytotoxic effects of capsidiol where pathogens do not gain entry during wound healing.

scholarly journals Archaeal Genome Guardians Give Insights into Eukaryotic DNA Replication and Damage Response Proteins

Archaea ◽  
2014 ◽  
Vol 2014 ◽  
pp. 1-24 ◽  
Author(s):  
David S. Shin ◽  
Ashley J. Pratt ◽  
John A. Tainer
Keyword(s):  
Dna Replication ◽  
Environmental Stress ◽  
Stress Responses ◽  
Protein Structures ◽  
Genome Integrity ◽  
Damage Response ◽  
Dna Replication And Repair ◽  
The Face ◽  
Domains Of Life ◽  
Fundamental Systems

As the third domain of life, archaea, like the eukarya and bacteria, must have robust DNA replication and repair complexes to ensure genome fidelity. Archaea moreover display a breadth of unique habitats and characteristics, and structural biologists increasingly appreciate these features. As archaea include extremophiles that can withstand diverse environmental stresses, they provide fundamental systems for understanding enzymes and pathways critical to genome integrity and stress responses. Such archaeal extremophiles provide critical data on the periodic table for life as well as on the biochemical, geochemical, and physical limitations to adaptive strategies allowing organisms to thrive under environmental stress relevant to determining the boundaries for life as we know it. Specifically, archaeal enzyme structures have informed the architecture and mechanisms of key DNA repair proteins and complexes. With added abilities to temperature-trap flexible complexes and reveal core domains of transient and dynamic complexes, these structures provide insights into mechanisms of maintaining genome integrity despite extreme environmental stress. The DNA damage response protein structures noted in this review therefore inform the basis for genome integrity in the face of environmental stress, with implications for all domains of life as well as for biomanufacturing, astrobiology, and medicine.

scholarly journals Dynamic changes in eIF4F-mRNA interactions revealed by global analyses of environmental stress responses

2017 ◽  
Vol 18 (1) ◽  
Author(s):  
Joseph L. Costello ◽  
Christopher J. Kershaw ◽  
Lydia M. Castelli ◽  
David Talavera ◽  
William Rowe ◽  
...  
Keyword(s):  
Environmental Stress ◽  
Stress Responses ◽  
Dynamic Changes

Stress responses as determinants of antimicrobial resistance in Pseudomonas aeruginosa: multidrug efflux and more

2014 ◽  
Vol 60 (12) ◽  
pp. 783-791 ◽  
Author(s):  
Keith Poole
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Resistance ◽  
Environmental Stress ◽  
Stress Responses ◽  
Acquired Resistance ◽  
Resistance Mechanisms ◽  
Resistant Organism ◽  
Multidrug Efflux ◽  
Resistance Development ◽  
Endogenous Genes

Pseudomonas aeruginosa is a notoriously antimicrobial-resistant organism that is increasingly refractory to antimicrobial chemotherapy. While the usual array of acquired resistance mechanisms contribute to resistance development in this organism a multitude of endogenous genes also play a role. These include a variety of multidrug efflux loci that contribute to both intrinsic and acquired antimicrobial resistance. Despite their roles in resistance, however, it is clear that these efflux systems function in more than just antimicrobial efflux. Indeed, recent data indicate that they are recruited in response to environmental stress and, therefore, function as components of the organism’s stress responses. In fact, a number of endogenous resistance-promoting genes are linked to environmental stress, functioning as part of known stress responses or recruited in response to a variety of environmental stress stimuli. Stress responses are, thus, important determinants of antimicrobial resistance in P. aeruginosa. As such, they represent possible therapeutic targets in countering antimicrobial resistance in this organism.

Sign in / Sign up

Export Citation Format

Share Document