Emission Rates and Characterization of Aerosols Produced During the Spreading of Dewatered Class B Biosolids

2007 ◽  
Vol 41 (10) ◽  
pp. 3537-3544 ◽  
Author(s):  
Tania Paez-Rubio ◽  
Abel Ramarui ◽  
Jeffrey Sommer ◽  
Hua Xin ◽  
James Anderson ◽  
...  
Keyword(s):  
2006 ◽  
Vol 40 (36) ◽  
pp. 7034-7045 ◽  
Author(s):  
Tania Paez-Rubio ◽  
Hua Xin ◽  
James Anderson ◽  
Jordan Peccia

2008 ◽  
Vol 52 (10) ◽  
pp. 3589-3596 ◽  
Author(s):  
Carlos Juan ◽  
Alejandro Beceiro ◽  
Olivia Gutiérrez ◽  
Sebastián Albertí ◽  
Margalida Garau ◽  
...  

ABSTRACT During a survey conducted to evaluate the incidence of class B carbapenemase (metallo-β-lactamase [MBL])-producing Pseudomonas aeruginosa strains from hospitals in Majorca, Spain, five clinical isolates showed a positive Etest MBL screening test result. In one of them, strain PA-SL2, the presence of a new bla VIM derivative (bla VIM-13) was detected by PCR amplification with bla VIM-1-specific primers followed by sequencing. The bla VIM-13-producing isolate showed resistance to all β-lactams (except aztreonam), gentamicin, tobramycin, and ciprofloxacin. VIM-13 exhibited 93% and 88% amino acid sequence identities with VIM-1 and VIM-2, respectively. bla VIM-13 was cloned in parallel with bla VIM-1, and the resistance profile conferred was analyzed both in Escherichia coli and in P. aeruginosa backgrounds. Compared to VIM-1, VIM-13 conferred slightly higher levels of resistance to piperacillin and lower levels of resistance to ceftazidime and cefepime. VIM-13 and VIM-1 were purified in parallel as well, and their kinetic parameters were compared. The k cat/K m ratios for the antibiotics mentioned above were in good agreement with the MIC data. Furthermore, EDTA inhibited the activity of VIM-13 approximately 25 times less than it inhibited the activity of VIM-1. VIM-13 was harbored in a class 1 integron, along with a new variant (Ala108Thr) of the aminoglycoside-modifying enzyme encoding gene aacA4, which confers resistance to gentamicin and tobramycin. Finally, the VIM-13 integron was apparently located in the chromosome, since transformation and conjugation experiments consistently yielded negative results and the bla VIM-13 probe hybridized only with the genomic DNA.


Lipids ◽  
2006 ◽  
Vol 41 (11) ◽  
pp. 1017-1027 ◽  
Author(s):  
E. J. Kleveland ◽  
B. L. Syvertsen ◽  
B. Ruyter ◽  
A. Vegusdal ◽  
S. M. Jørgensen ◽  
...  

2001 ◽  
Vol 16 (4) ◽  
pp. 471-481 ◽  
Author(s):  
R. A. Wadden ◽  
Maryann Suero ◽  
L. M. Conroy ◽  
J. E. Franke ◽  
P. A. Scheff
Keyword(s):  

2006 ◽  
Vol 146 (2) ◽  
pp. 191-198 ◽  
Author(s):  
Maria Cristina Thaller ◽  
Serena Schippa ◽  
Alessandra Bonci ◽  
Stefania Cresti ◽  
Gian Maria Rossolini

2011 ◽  
Vol 56 (1) ◽  
pp. 588-590 ◽  
Author(s):  
Takehisa Matsumoto ◽  
Mika Nagata ◽  
Nau Ishimine ◽  
Kenji Kawasaki ◽  
Kazuyoshi Yamauchi ◽  
...  

ABSTRACTAn Ambler class A β-lactamase gene,blaCIA-1, was cloned from the reference strainChryseobacterium indologenesATCC 29897 and expressed inEscherichia coliBL21. TheblaCIA-1gene encodes a novel extended-spectrum β-lactamase (ESBL) that shared 68% and 60% identities with the CGA-1 and CME-1 β-lactamases, respectively.blaCIA-1-like genes were detected from clinical isolates. In addition to the metallo-β-lactamase IND of Ambler class B,C. indologeneshas a class A ESBL gene,blaCIA-1, located on the chromosome.


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