Phylogenetic Characterization of Microbial Communities That Reductively Dechlorinate TCE Based upon a Combination of Molecular Techniques

2002 ◽  
Vol 36 (12) ◽  
pp. 2652-2662 ◽  
Author(s):  
Ruth E. Richardson ◽  
Vishvesh K. Bhupathiraju ◽  
Donald L. Song ◽  
Tanuja A. Goulet ◽  
Lisa Alvarez-Cohen
Keyword(s):  
Microbial Communities ◽  
Molecular Techniques ◽  
Phylogenetic Characterization

scholarly journals Characterization of Ocular Surface Microbial Profiles Revealed Discrepancies between Conjunctival and Corneal Microbiota

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 405
Author(s):  
Anna Matysiak ◽  
Michal Kabza ◽  
Justyna A. Karolak ◽  
Marcelina M. Jaworska ◽  
Malgorzata Rydzanicz ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Ocular Surface ◽  
Molecular Techniques ◽  
Corneal Tissue ◽  
Rna Seq ◽  
Microbiome Composition ◽  
Viral Sequences ◽  
Pcr Techniques ◽  
Unmapped Reads

The ocular microbiome composition has only been partially characterized. Here, we used RNA-sequencing (RNA-Seq) data to assess microbial diversity in human corneal tissue. Additionally, conjunctival swab samples were examined to characterize ocular surface microbiota. Short RNA-Seq reads, obtained from a previous transcriptome study of 50 corneal tissues, were mapped to the human reference genome GRCh38 to remove sequences of human origin. The unmapped reads were then used for taxonomic classification by comparing them with known bacterial, archaeal, and viral sequences from public databases. The components of microbial communities were identified and characterized using both conventional microbiology and polymerase chain reaction (PCR) techniques in 36 conjunctival swabs. The majority of ocular samples examined by conventional and molecular techniques showed very similar microbial taxonomic profiles, with most of the microorganisms being classified into Proteobacteria, Firmicutes, and Actinobacteria phyla. Only 50% of conjunctival samples exhibited bacterial growth. The PCR detection provided a broader overview of positive results for conjunctival materials. The RNA-Seq assessment revealed significant variability of the corneal microbial communities, including fastidious bacteria and viruses. The use of the combined techniques allowed for a comprehensive characterization of the eye microbiome’s elements, especially in aspects of microbiota diversity.

scholarly journals Molecular-phylogenetic characterization of microbial communities imbalances in the small intestine of dogs with inflammatory bowel disease

2008 ◽  
Vol 66 (3) ◽  
pp. 579-589 ◽  
Author(s):  
Panagiotis G. Xenoulis ◽  
Blake Palculict ◽  
Karin Allenspach ◽  
Jörg M. Steiner ◽  
Angela M. Van House ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Small Intestine ◽  
Microbial Communities ◽  
Bowel Disease ◽  
Phylogenetic Characterization ◽  
Molecular Phylogenetic ◽  
Inflammatory Bowel

scholarly journals Phylogenetic Characterization of Fecal Microbial Communities of Dogs Fed Diets with or without Supplemental Dietary Fiber Using 454 Pyrosequencing

PLoS ONE ◽  
2010 ◽  
Vol 5 (3) ◽  
pp. e9768 ◽  
Author(s):  
Ingmar S. Middelbos ◽  
Brittany M. Vester Boler ◽  
Ani Qu ◽  
Bryan A. White ◽  
Kelly S. Swanson ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Dietary Fiber ◽  
454 Pyrosequencing ◽  
Phylogenetic Characterization

scholarly journals Metagenomic Characterization of Microbial Communities In Situ Within the Deeper Layers of the Ileum in Crohn’s Disease

2016 ◽  
Vol 2 (5) ◽  
pp. 563-566.e5 ◽  
Author(s):  
Chandra Sekhar Pedamallu ◽  
Ami S. Bhatt ◽  
Susan Bullman ◽  
Sharyle Fowler ◽  
Samuel S. Freeman ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Microbial Communities

DETECTION AND PHYLOGENETIC CHARACTERIZATION OF HEPATITIS E VIRUS GENOTYPE 3 IN A CAPTIVE WILD BOAR IN THAILAND

2012 ◽  
Vol 43 (3) ◽  
pp. 640-644 ◽  
Author(s):  
Anuwat Wiratsudakul ◽  
Ladawan Sariya ◽  
Phirom Prompiram ◽  
Siriporn Tantawet ◽  
Duangkhamol Suraruangchai ◽  
...  
Keyword(s):  
Wild Boar ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genotype 3 ◽  
Virus Genotype ◽  
Phylogenetic Characterization
Sign in / Sign up

Export Citation Format

Share Document