Molecular weight distribution of dissolved organic carbon and dissolved organic halogen in advanced treated wastewaters

1984 ◽  
Vol 18 (6) ◽  
pp. 410-415 ◽  
Author(s):  
Martin. Reinhard
Keyword(s):  
Molecular Weight ◽  
Organic Carbon ◽  
Dissolved Organic Carbon ◽  
Molecular Weight Distribution ◽  
Weight Distribution ◽  
Organic Halogen

Effect of O3 and O3/H2O2 on algae harvesting using chitosan

2013 ◽  
Vol 67 (6) ◽  
pp. 1294-1301 ◽  
Author(s):  
R. Pranowo ◽  
D. J. Lee ◽  
J. C. Liu ◽  
J. S. Chang
Keyword(s):  
Hydrogen Peroxide ◽  
Molecular Weight ◽  
Organic Matter ◽  
Organic Carbon ◽  
Dissolved Organic Carbon ◽  
Weight Distribution ◽  
Separation Efficiency ◽  
Polyaluminum Chloride ◽  
Floc Size ◽  
Extracellular Organic Matter

We examined the effects of pre-oxidation using ozone (O3) and a combination of O3 and hydrogen peroxide (O3/H2O2) on algae suspensions and their harvesting. Inactivation of algae cells, release of intracellular organic matter (IOM), mineralization of extracellular organic matter (EOM), and changes in molecular weight distribution of EOM were found after pre-oxidation. Enhanced separation efficiency of turbidity, dissolved organic carbon (DOC), protein, and polysaccharide using chitosan and polyaluminum chloride (PACl) was found after pre-oxidation, especially when algae cells were subject to O3/H2O2. Chitosan showed higher efficiency than PACl. Judging from the remarkable increase in floc size, it was proposed that released IOM formed complexes with cationic chitosan and resulted in enhanced dual flocculation and facilitated algae separation.

Extraction and Partial Purification of Protease from Bilimbi (Averrhoa bilimbi L.)

2013 ◽  
Vol 10 (2) ◽  
pp. 29
Author(s):  
Normah Ismail ◽  
Nur' Ain Mohamad Kharoe
Keyword(s):  
Molecular Weight ◽  
Protein Content ◽  
Ammonium Sulfate ◽  
Molecular Weight Distribution ◽  
Weight Distribution ◽  
Protease Activity ◽  
Protein Concentration ◽  
Temperature Stability ◽  
Partial Purification ◽  
Ammonium Sulfate Precipitation

Unripe and ripe bilimbi (Averrhoa bilimbi L.) were ground and the extracted juices were partially purified by ammonium sulfate precipitation at the concentrations of 40 and 60% (w/v). The collected proteases were analysed for pH, temperature stability, storage stability, molecular weight distribution, protein concentration and protein content. Protein content of bilimbi fruit was 0.89 g. Protease activity of both the unripe and ripe fruit were optimum at pH 4 and 40°C when the juice were purified at 40 and 60% ammonium sulfate precipitation. A decreased in protease activity was observed during the seven days of storage at 4°C. Molecular weight distribution indicated that the proteases protein bands fall between IO to 220 kDa. Protein bands were observed at 25, 50 and 160 kDa in both the unripe and ripe bilimbi proteases purified with 40% ammonium sulfate, however, the bands were more intense in those from unripe bilimbi. No protein bands were seen in proteases purified with 60% ammonium sulfate. Protein concentration was higher for proteases extracted with 40% ammonium sulfate at both ripening stages. Thus, purification using 40% ammonium sulfate precipitation could be a successful method to partially purify proteases from bilimbi especially from the unripe stage. 

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