Detection of the cp4 epsps Gene in Maize Line NK603 and Comparison of Related Protein Structures: An Advanced Undergraduate Experiment

2015 ◽  
Vol 92 (7) ◽  
pp. 1229-1232 ◽  
Author(s):  
Nicole K. Swope ◽  
Patrick J. Fryfogle ◽  
Tami L. Sivy
Keyword(s):  
Protein Structures ◽  
Related Protein ◽  
Maize Line ◽  
Epsps Gene ◽  
Cp4 Epsps Gene ◽  
Cp4 Epsps

Comparison of generational effect on proteins and metabolites in non-transgenic and transgenic soybean seeds through the insertion of the cp4-EPSPS gene assessed by omics-based platforms

2020 ◽  
Vol 202 ◽  
pp. 110918
Author(s):  
Bruna K. de Campos ◽  
Rodrigo M. Galazzi ◽  
Bruna M. dos Santos ◽  
Tiago S. Balbuena ◽  
Fábio N. dos Santos ◽  
...  
Keyword(s):  
Soybean Seeds ◽  
Transgenic Soybean ◽  
Epsps Gene ◽  
Cp4 Epsps Gene ◽  
Cp4 Epsps

scholarly journals Final Health and Environmental Risk Assessment of Genetically Modified Soybean MON 89788

2021 ◽  
pp. 134-135
Author(s):  
Åshild Kristin Andreassen ◽  
Anne Marie Bakke ◽  
Knut Kelkås Dahl ◽  
Knut Thomas Dalen ◽  
Merethe Aasmo Finne ◽  
...  
Keyword(s):  
Nutritional Assessment ◽  
Current Knowledge ◽  
Animal Health ◽  
Field Trials ◽  
Genetically Modified Soybean ◽  
Bioinformatics Analyses ◽  
Epsps Gene ◽  
Flanking Sequences ◽  
Cp4 Epsps Gene ◽  
Cp4 Epsps

Soybean MON 89788 expresses the cp4 epsps gene from the plant pathogenic bacterium Agrobacterium tumefaciens (Rhizobium radiobacter) sp. strain CP4. The encoded enzyme 5enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) protein confers tolerance to the active herbicidal substance glyphosate. Updated bioinformatics analyses of the inserted DNA and flanking sequences in soybean MON 89788 have not indicated a potential production of putative harmful proteins or polypeptides caused by the genetic modification. Genomic stability of the functional insert and consistent expression of the cp4 epsps gene, have been shown over several generations of soybean MON 89788. With the exception of the intended changes caused by the trans-genetically introduced trait, data from several field trials performed in USA and Argentina show that soybean MON 89788 is compositionally, morphologically and agronomically equivalent to its conventional counterpart and other commercial soybean varieties. A sub-chronic feeding study with rats, as well as a nutritional assessment trial with broilers has not revealed adverse effects of soybean MON 89788. These studies indicate that soybean MON 89788 is nutritionally equivalent to, and as safe as conventional soybean varieties. The CP4 EPSPS protein produced in soybean MON 89788 does not show sequence resemblance to known toxins or IgE-dependent allergens, nor has it been reported to cause IgE-mediated allergic reactions. Soybean is not cultivated in Norway, and there are no cross-compatible wild or weedy relatives of soybean in Europe.    Based on current knowledge, the VKM GMO Panel concludes that with the intended usage, there are no discernible safety concerns associated with soybean MON 89788 regarding human or animal health or to the environment in Norway.

scholarly journals Expression patterns of cp4-epsps gene in diverse transgenic Saccharum officinarum L. genotypes

2019 ◽  
Vol 25 (3) ◽  
pp. 779-786
Author(s):  
Muhammad Imran ◽  
Andre Luiz Barboza ◽  
Shaheen Asad ◽  
Zafar M. Khalid ◽  
Zahid Mukhtar
Keyword(s):  
Expression Patterns ◽  
Saccharum Officinarum ◽  
Epsps Gene ◽  
Cp4 Epsps Gene ◽  
Cp4 Epsps

scholarly journals Development of Gene-Specific Real-Time PCR Screening Method for Detection of cp4-epsps Gene in GM Crops

2017 ◽  
Author(s):  
Solmaz Khosravi ◽  
Masoud Tohidfar ◽  
Parisa Koobaz
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Limit Of Detection ◽  
Screening Method ◽  
Gm Crops ◽  
Pcr Screening ◽  
Herbicide Resistant ◽  
Epsps Gene ◽  
Cp4 Epsps Gene ◽  
Cp4 Epsps

AbstractAmong the genetically modified (GM) crops that are being approved for commercialization, herbicide resistant crops, especially those harboring cp4-epsps, have a considerable contribution. Gene-specific methods can be used to screen the presence of GMOs. To establish an effective qualitative and quantitative screening method, a set of primers were designed considering the cp4-epsps sequence. The specificity, the limit of detection, the efficiency, and the ability to quantify the GMO content were tested in GM cotton, soybean, and canola events. The results demonstrated that the primers can specifically detect cp4-epsps GM crops. The limit of detection was found to be 0.4 ng /μl DNA per PCR reaction with the ability to detect 1-16 copies of the haploid genome of each GM event. The efficiency of this screening method (which was 94-110 % with an R2 higher than 0.96) indicated that these new primers can be applied to the screening of GM samples that contain the cp4-epsps gene. Also, the gene-specific real-time PCR screening method could be successfully developed for qualification of different types of GM cotton, soybean and canola events with the construction of a serial dilution ranging from 10 % to 1 %.

scholarly journals Standardization of PCR and sequencing-based methods for the detection of the presence of CP4 EPSPS gene in Zea mays

2020 ◽  
Vol 12 ◽  
pp. 1-9
Author(s):  
Carlos Humberto Chamone Cangussu ◽  
Léia Cardoso ◽  
Alessandra Rejane Ericsson de Oliveira Xavier ◽  
Josiane dos Santos ◽  
Luiz Felipe Lopes Campos ◽  
...  
Keyword(s):  
Zea Mays ◽  
Transgenic Maize ◽  
Transgenic Event ◽  
Epsps Gene ◽  
Maize Cultivars ◽  
Qualitative Detection ◽  
Pcr Products ◽  
Cp4 Epsps Gene ◽  
Cp4 Epsps ◽  
Herbicide Glyphosate

With the rise in planting of transgenic cultivars in Brazil as well as worldwide, the demand for legal regulations has increased. The transgenic event MON88017 is often found in maize cultivars marketed in Brazil. The event contains the CP4 EPSPS and cry3Bb1 genes, which encode tolerance to the herbicide glyphosate and resistance to caterpillars, respectively. Globally, methodologies to track transgenic events are mandatory. The objective of this study was to standardize a method based on qualitative PCR and sequencing for detection of the CP4 EPSPS gene in Zea mays. DNA was extracted from three commercial strains of transgenic maize, containing the MON88017 event, as well as conventional maize. Primers were designed for partial detection of the zein and CP4 EPSPS genes. PCR reactions were performed for detection of partial regions of CP4 EPSPS and Zein (as endogenous marker of Z. mays) genes. The three transgenic maize inbred lines tested positive for zein and CP4 EPSPS, and the two conventional strains tested negative for CP4 EPSPS and positive for the Zein gene. To confirm the presence of the genic regions, PCR products were sequenced and showed 100% identity with sequences of Zein and CP4 EPSPS genes deposited in GenBank. Thus, the results of this study suggest the applicability of an ‘in-house’ method for the qualitative detection of CP4 EPSPS in genetically modified maize cultivars.

Ionomics and lipidomics for evaluating the transgenic (cp4-EPSPS gene) and non-transgenic soybean seed generations

2021 ◽  
pp. 106130
Author(s):  
Luana Ferreira da Costa ◽  
Claúdio Francisco Tormena ◽  
Marco Aurélio Zezzi Arruda
Keyword(s):  
Soybean Seed ◽  
Transgenic Soybean ◽  
Epsps Gene ◽  
Cp4 Epsps Gene ◽  
Cp4 Epsps
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