The Heteronuclear Single-Quantum Correlation (HSQC) Experiment: Vectors versus Product Operators

2014 ◽  
Vol 92 (3) ◽  
pp. 482-487 ◽  
Author(s):  
Karen de la Vega-Hernández ◽  
Manuel Antuch
Keyword(s):  
Quantum Correlation ◽  
Single Quantum ◽  
Heteronuclear Single Quantum Correlation

scholarly journals Proton-detected heteronuclear single quantum correlation NMR spectroscopy in rigid solids with ultra-fast MAS

2010 ◽  
Vol 202 (1) ◽  
pp. 64-71 ◽  
Author(s):  
Gregory P. Holland ◽  
Brian R. Cherry ◽  
Janelle E. Jenkins ◽  
Jeffery L. Yarger
Keyword(s):  
Nmr Spectroscopy ◽  
Quantum Correlation ◽  
Single Quantum ◽  
Fast Mas ◽  
Heteronuclear Single Quantum Correlation

scholarly journals CARACTERIZACIÓN DE UNA ESPIROLACTONA SESQUITERPÉNICA á-METILÉNICA OBTENIDA DE Ambrosia arborescens Miller Y EVALUACIÓN DE SU ACTIVIDAD BIOLÓGICA EN Tripanosoma cruzi

2014 ◽  
Vol 80 (2) ◽  
pp. 124-135
Author(s):  
Teresa Cano de Terrones
Keyword(s):  
Quantum Correlation ◽  
Multiple Bond ◽  
Correlation Spectroscopy ◽  
Single Quantum ◽  
Heteronuclear Multiple Bond Correlation ◽  
Heteronuclear Single Quantum Correlation ◽  
América Del Sur

Ambrosia arborescens Miller, denominada comúnmente "marco"; es una planta aromática, medicinal que se encuentra en América del Sur, mayormente en los Andes del Perú, en el Departamento de Arequipa. El estudio de los constituyentes químicos de la parte aérea de Ambrosia arborescens permitió obtener una espirolactona sesquiterpénica. Para su separación y aislamiento se utilizó métodos cromatográficos de columna, capa fina y cromatografía líquida de alta eficacia. La elucidación estructural se realizó a través de métodos espectroscópicos: resonancia magnética nuclear protónica (RMN-1H), del carbono-13 (RMN-13C), espectroscopía infrarroja (IR) y espectrometría de masas (EM). En la determinación de la estereoquímica y las conformaciones se empleó experimentos bidimensionales de espectroscopía: COSY (Correlation Spectroscopy), HSQC (Heteronuclear Single Quantum Correlation), HMBC (Heteronuclear Multiple Bond Correlation) y ROESY(Rotational nuclear Overhouser Effect Spectroscopy). Desde un punto de vista teórico, el análisis conformacional fue realizado a través de Mecánica Molecular empleando el programa MM2 de Hyperchem. La evaluación de la actividad antiparasitaria, in vitro, sobre Tripanosoma cruzi, mostró que a muy bajas concentraciones, el producto obtenido presenta actividad tripanocida.

scholarly journals Robust detection of oncometabolic aberrations by 1H–13C heteronuclear single quantum correlation in intact biological specimens

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Yasaman Barekatain ◽  
Victoria C. Yan ◽  
Kenisha Arthur ◽  
Jeffrey J. Ackroyd ◽  
Sunada Khadka ◽  
...  
Keyword(s):  
Quantum Correlation ◽  
Ex Vivo ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Precision Oncology ◽  
Single Quantum ◽  
Diagnostic Significance ◽  
Primary Tumors ◽  
Robust Detection ◽  
Heteronuclear Single Quantum Correlation

AbstractMagnetic resonance (MR) spectroscopy has potential to non-invasively detect metabolites of diagnostic significance for precision oncology. Yet, many metabolites have similar chemical shifts, yielding highly convoluted 1H spectra of intact biological material and limiting diagnostic utility. Here, we show that hydrogen–carbon heteronuclear single quantum correlation (1H–13C HSQC) offers dramatic improvements in sensitivity compared to one-dimensional (1D) 13C NMR and significant signal deconvolution compared to 1D 1H spectra in intact biological settings. Using a standard NMR spectroscope with a cryoprobe but without specialized signal enhancing features such as magic angle spinning, metabolite extractions or 13C-isotopic enrichment, we obtain well-resolved 2D 1H–13C HSQC spectra in live cancer cells, in ex vivo freshly dissected xenografted tumors and resected primary tumors. This method can identify tumors with specific oncometabolite alterations such as IDH mutations by 2-hydroxyglutarate and PGD-deleted tumors by gluconate. Results suggest potential of 1H–13C HSQC as a non-invasive diagnostic in precision oncology.

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