Low-Cost Method for Quantifying Sodium in Coconut Water and Seawater for the Undergraduate Analytical Chemistry Laboratory: Flame Test, a Mobile Phone Camera, and Image Processing

2014 ◽  
Vol 91 (11) ◽  
pp. 1958-1960 ◽  
Author(s):  
Edgar P. Moraes ◽  
Nilbert S. A. da Silva ◽  
Camilo de L. M. de Morais ◽  
Luiz S. das Neves ◽  
Kassio M. G. de Lima
PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e95330 ◽  
Author(s):  
Neil A. Switz ◽  
Michael V. D'Ambrosio ◽  
Daniel A. Fletcher

Water ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 1025
Author(s):  
Wanda Guedens ◽  
Monique Reynders ◽  
Koen Van Vinckenroye ◽  
Jan Yperman ◽  
Robert Carleer

In analytical chemistry laboratories, to gather in the shortest time as many data as possible with the utmost accuracy and precision, high throughput automated setups are indispensable. In the present study, to determine the chloride concentration in the international Scheldt river basin district, experiments are carried out utilizing a thermostatically controlled semi-automated setup. A novel ICT-based method is developed using a low-cost multifunction Data Acquisition Board (DAQ) controlled by a homebuilt LabVIEW™ program. Specifically, this approach enables a correlation between different parameters i.e., droplet volume, temperature, A/D voltage conversions. Here, processing experimental data of a potentiometric precipitation titration utilizing a silver nitrate standard solution as titrant in a manual burette equipped with a controllable electronic valve allows for a preliminary indication of the titration end point via the Virtual Instrument (VI) numerical first derivative tool in the LabVIEW software. The LabVIEW tool is compared with the well-known Gran method implemented in the LabVIEW program, emphasizing an accurate performance of the setup to determine the chloride concentration in fresh river water. We are confident that our findings are evidence of the versatile and powerful features of the LabVIEW controlled DAQ in the analytical chemistry laboratory.


2021 ◽  
Vol 22 (1) ◽  
pp. 79-85
Author(s):  
Koekoeh Santoso ◽  
Ulfatin Khoiriyah Herowati ◽  
Dordia Anindita Rotinsulu ◽  
Sri Murtini ◽  
Muhammad Yusuf Ridwan ◽  
...  

Rabies is an infectious disease, zoonotic, caused by virus from the genus Lyssa virus and generally transmitted by the bite of rabid animal, especially rabies infected dog. Rabies is preventable but is always fatal to humans if the central nervous system (CNS) is infected. Vaccination has been used as one of rabies prevention programmed. A total of 83 samples were tested using an Indirect ELISA (Enzyme Linked Immunosorbent Assay) to identify post-vaccination rabies antibody titer. Antibody titres correlated with absorbance values and standard solutions concentrations. Absorbance value can be determined using ELISA reader and mobile phone camera. Absorbance were read at 450 nm and 620 nm as reference using ELISA reader and image from mobile phone camera using image processing software (ImageJ). The aim of this study is to compared between ELISA reader as gold standard and mobile phone camera through validity testing such sensitivity, specificity, and accuracy. There is no significant difference between gold standard and alternative test equipment. The mobile phone camera has sensitivity 98,6%, specificity 88.8 % and accuracy 97,5%. The image processing method using ELISA reader is relatively expensive and difficult to hold in laboratory with minimum funds. Image processing method using a mobile phone camera with ImageJ application is expected to be an alternative tool to read the result of ELISA.


2010 ◽  
Vol 4 ◽  
pp. SART.S5025
Author(s):  
Brian A. Cadle ◽  
Kristin C. Rasmus ◽  
Juan A. Varela ◽  
Leah S. Leverich ◽  
Casey E. O'Neill ◽  
...  

Here we describe the first report of using low-cost cellular or web-based digital cameras to image and quantify standardized rapid immunoassay strips as a new point-of-care diagnostic and forensics tool with health applications. Quantitative ratiometric pixel density analysis (QRPDA) is an automated method requiring end-users to utilize inexpensive (~ $1 USD/each) immunotest strips, a commonly available web or mobile phone camera or scanner, and internet or cellular service. A model is described whereby a central computer server and freely available IMAGEJ image analysis software records and analyzes the incoming image data with time-stamp and geo-tag information and performs the QRPDA using custom JAVA based macros ( http://www.neurocloud.org ). To demonstrate QRPDA we developed a standardized method using rapid immunotest strips directed against cocaine and its major metabolite, benzoylecgonine. Images from standardized samples were acquired using several devices, including a mobile phone camera, web cam, and scanner. We performed image analysis of three brands of commercially available dye-conjugated anti-cocaine/benzoylecgonine (COC/BE) antibody test strips in response to three different series of cocaine concentrations ranging from 0.1 to 300 ng/ml and BE concentrations ranging from 0.003 to 0.1 ng/ml. This data was then used to create standard curves to allow quantification of COC/BE in biological samples. Across all devices, QRPDA quantification of COC and BE proved to be a sensitive, economical, and faster alternative to more costly methods, such as gas chromatography-mass spectrometry, tandem mass spectrometry, or high pressure liquid chromatography. The limit of detection was determined to be between 0.1 and 5 ng/ml. To simulate conditions in the field, QRPDA was found to be robust under a variety of image acquisition and testing conditions that varied temperature, lighting, resolution, magnification and concentrations of biological fluid in a sample. To determine the effectiveness of the QRPDA method for quantifying cocaine in biological samples, mice were injected with a sub-locomotor activating dose of cocaine (5 mg/kg; i.p.) and were found to have detectable levels of COC/BE in their urine (160.6 ng/ml) and blood plasma (8.1 ng/ml) after 15–30 minutes. By comparison rats self-administering cocaine in a 4 hour session obtained a final BE blood plasma level of 910 ng/ml with an average of 62.5 infusions. It is concluded that automated QRPDA is a low-cost, rapid and highly sensitive method for the detection of COC/BE with health, forensics, and bioinformatics application and the potential to be used with other rapid immunotest strips directed at several other targets. Thus, this report serves as a general reference and method describing the use of image analysis of lateral flow rapid test strips.


2020 ◽  
Vol 2020 (15) ◽  
pp. 350-1-350-10
Author(s):  
Yin Wang ◽  
Baekdu Choi ◽  
Davi He ◽  
Zillion Lin ◽  
George Chiu ◽  
...  

In this paper, we will introduce a novel low-cost, small size, portable nail printer. The usage of this system is to print any desired pattern on a finger nail in just a few minutes. The detailed pre-processing procedures will be described in this paper. These include image processing to find the correct printing zone, and color management to match the patterns’ color. In each phase, a novel algorithm will be introduced to refine the result. The paper will state the mathematical principles behind each phase, and show the experimental results, which illustrate the algorithms’ capabilities to handle the task.


2020 ◽  
Vol 52 (4) ◽  
pp. 726-732
Author(s):  
Claire Beaugrand

In a tweet posted on 29 March 2018, a bidūn activist—who was later jailed from July 2019 to January 2020 for peacefully protesting against the inhumane conditions under which the bidūn are living—shared a video. The brief video zooms in closely on an ID card, recognizable as one of those issued to the bidūn, or long-term residents of Kuwait who are in contention with the state regarding their legal status. More precisely, the mobile phone camera focuses on the back of the ID card, on one line with a special mention added by the Central System (al-jihāz al-markazī), the administration in charge of bidūn affairs. Other magnetic strip cards hide the personal data written above and below it. A male voice can be heard saying that he will read this additional remark, but before even doing so he bursts into laughter. The faceless voice goes on to read out the label in an unrestrained laugh: “ladayh qarīb … ladayh qarīna … dālla ʿalā al-jinsiyya al-ʿIrāqiyya” (he has a relative … who has presumptive evidence … suggesting an Iraqi nationality). The video shakes as the result of a contagious laugh that grows in intensity. In the Kuwaiti dialect, the voice continues commenting: “Uqsim bil-Allāh, gaʿadt sāʿa ufakkir shinū maʿanāt hal-ḥatchī” (I swear by God, it took me an hour to figure out the meaning of this nonsense), before reading the sentence again, stopping and guffawing, and asking if he should “repeat it a third time,” expressing amazement at its absurdity. The tweet, addressed to the head of the Central System (mentioned in the hashtag #faḍīḥat Sāliḥ al-Faḍāla, or #scandal Salih al-Fadala), reads: In lam tastaḥī fa-'ktub mā shaʾt (Don't bother, write what you want).


IEEE Access ◽  
2018 ◽  
Vol 6 ◽  
pp. 53053-53061 ◽  
Author(s):  
Zhenshan Zhang ◽  
Tiantian Zhang ◽  
Ji Zhou ◽  
Yueming Lu ◽  
Yaojun Qiao

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