Determination of Surface Coverage of an Adsorbate on Silica Using FTIR Spectroscopy

1999 ◽  
Vol 76 (2) ◽  
pp. 253 ◽  
Author(s):  
Jeanne E. Pemberton ◽  
Laurie L. Wood ◽  
Ghanshyam S. Ghoman
Keyword(s):  
Ftir Spectroscopy ◽  
Surface Coverage

A novel approach for the discrimination of culture medium from Vascular Endothelial Growth Factor (VEGF) overexpressing colorectal cancer cells

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Sinem Tunçer ◽  
Rafig Gurbanov
Keyword(s):  
Colorectal Cancer ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Ftir Spectroscopy ◽  
Cancer Cells ◽  
Lower Amount ◽  
Vascular Endothelial ◽  
Colorectal Cancer Cells ◽  
Endothelial Growth Factor

AbstractObjectivesThe expression level of Vascular Endothelial Growth Factor (VEGF) is assumed as a prognostic marker for several tumor types, including colorectal cancer. Therefore, the determination of pre- and post-therapy levels of VEGF appears to have great value in the assessment of tumor prognosis. Enzyme-Linked Immunosorbent Assay (ELISA) is commonly used for the determination of serum or plasma VEGF levels, but the method is costly and time-consuming. In this study, we aimed to describe a rapid and cost-effective analysis method to discriminate VEGF overexpressing colorectal cancer-derived conditioned medium (CM).MethodsAttenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, combined with Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA), was used to differentiate VEGF overexpressing colorectal cancer cell line CM from CM obtained from the corresponding control cells which express and secrete relatively lower amount of VEGF.ResultsSamples belong to VEGF overexpressing colorectal cancer cells were clearly distinguished from the control group with very high PC scores as PC1 + PC2 = 96%. Besides, a 100% accurate distinction between these two groups was achieved by the LDA analysis.ConclusionsATR-FTIR spectroscopy combined with pattern recognition techniques was able to discriminate CM of VEGF overexpressing colorectal cancer cells with high efficiency and accuracy.

Determination of Total Polar Compounds in Frying Oils by PE-Film-Based FTIR and ATR-FTIR Spectroscopy

2018 ◽  
Vol 120 (12) ◽  
pp. 1800250 ◽  
Author(s):  
Jia Chen ◽  
Leshan Zhang ◽  
Qiaona Geng ◽  
Bingyu Jing ◽  
Xiuzhu Yu
Keyword(s):  
Ftir Spectroscopy ◽  
Polar Compounds ◽  
Total Polar Compounds ◽  
Frying Oils

Surface Structure and Cells Adhesion on Doped Polyethylene

2007 ◽  
Vol 567-568 ◽  
pp. 253-256
Author(s):  
Regina Mikulíková ◽  
Kateřina Kolářová ◽  
Václav Švorčík ◽  
Barbora Dvořánková ◽  
Tomáš Sopuch
Keyword(s):  
Surface Morphology ◽  
Ftir Spectroscopy ◽  
Crystalline Phase ◽  
Film Surface ◽  
Surface Wettability ◽  
Oxidized Cellulose ◽  
3T3 Fibroblasts ◽  
Doped Polymer

The properties of polyethylene doped with Ca2+ salt of oxidized cellulose was studied by different techniques. FTIR spectroscopy was used for the determination of crystalline phase in polymer film, surface wettability was determined by standard goniometry and surface morphology was examined by SEM microscopy. Adhesion of mouse 3T3 fibroblasts on the doped polymer was studied in vitro. It was found that the polyethylene doped with the cellulose derivative can be sterilized in boiling water. The number and homogeneity of adhering cells were shown to depend on the surface wettability and morphology.

Direct determination of niflumic acid in a pharmaceutical gel by ATR/FTIR spectroscopy and PLS calibration

2006 ◽  
Vol 40 (2) ◽  
pp. 433-437 ◽  
Author(s):  
C. Boyer ◽  
B. Brégère ◽  
S. Crouchet ◽  
K. Gaudin ◽  
J.P. Dubost
Keyword(s):  
Ftir Spectroscopy ◽  
Direct Determination ◽  
Niflumic Acid
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