A simple method for spectrophotometric determination of two-components with overlapped spectra

1989 ◽  
Vol 66 (2) ◽  
pp. 178 ◽  
Author(s):  
M. Blanco ◽  
H. Iturriaga ◽  
S. Maspoch ◽  
P. Tarin
Keyword(s):  
Spectrophotometric Determination ◽  
Simple Method

Simple spectrophotometric determination of sulfate free radicals

2018 ◽  
Vol 10 (28) ◽  
pp. 3470-3474 ◽  
Author(s):  
Chen Wang ◽  
Rui Chen ◽  
Ruyu Zhang ◽  
Naidong Zhang
Keyword(s):  
Free Radicals ◽  
Spectrophotometric Determination ◽  
Sulfate Radical ◽  
Light Sources ◽  
Sulfate Radicals ◽  
Simple Method

A rapid and simple method for sulfate radical determination was described and the generation rates of sulfate radicals generated by photolysis of persulfate under different light sources were studied.

scholarly journals A simple method for the spectrophotometric determination of cephalosporins in pharmaceuticals using variamine blue

2018 ◽  
Vol 33 (2) ◽  
pp. 41
Author(s):  
Chand Pasha ◽  
Badiadka Narayana
Keyword(s):  
Sodium Hydroxide ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Acidic Medium ◽  
Simple Method ◽  
Lactam Ring ◽  
Ph Range ◽  
Variamine Blue ◽  
Hydrolysis Of

A simple spectrophotometric method for the determination of cefotaxime, ceftriaxone, cefadroxil and cephalexin with variamine blue is presented. The determination is based on the hydrolysis of β-lactam ring of cephalosporins with sodium hydroxide which subsequently reacts with iodate to liberate iodine in acidic medium. The liberated iodine oxidizes variamine blue to violet colored species of maximum absorption at 556 nm. The absorbance is measured within the pH range of 4.0-4.2. Beer’s law is obeyed in the range of 0.5-5.8 μg mL–1, 0.2-7.0 μg mL–1, 0.2-5.0 μg mL–1 and 0.5-8.5 μg mL–1 for cefotaxime, ceftriaxone, cefadroxil and cephalexin respectively. The analytical parameters were optimized and the method is successfully applied for the determination of cefotaxime, ceftriaxone, cefadroxil and cephalexin in pharmaceuticals.

A simple method for the spectrophotometric determination of chloride by solvent extraction as chromyl chloride

1977 ◽  
Vol 67 (1-2) ◽  
pp. 29-35 ◽  
Author(s):  
C. Gonzalez Perez ◽  
L. Polo Diez ◽  
A. Sanchez Perez ◽  
F. Lucena Conde
Keyword(s):  
Solvent Extraction ◽  
Spectrophotometric Determination ◽  
Simple Method ◽  
Chromyl Chloride

Spectrophotometric Determination of Total Macromolecules for the Evaluation of Cellular Density in Cytotoxicity Tests

1991 ◽  
Vol 19 (1) ◽  
pp. 18-25
Author(s):  
Elisabetta Fortunati ◽  
Vera Bianchi
Keyword(s):  
Cell Density ◽  
Spectrophotometric Determination ◽  
Neutral Red ◽  
Cell Number ◽  
Cell Viability Assay ◽  
Simple Method ◽  
Cellular Density ◽  
Viability Assay ◽  
Uptake Test

Variation of cell number in treated cultures relative to the controls is an indicator of toxicity frequently employed in cytotoxicity tests. Cell density is generally determined from the amount of macromolecular components (DNA, proteins) measured by colorimetric methods. Another established procedure is the neutral red (NR) uptake test, which, in principle, permits an evaluation of the amount of viable cells in the different samples. We have devised a simple method for comparing cell density in the cultures, based on the spectrophotometric determination of the total macromolecules present in the cell monolayers solubilised in alkali after removal of the soluble fraction (TM test). When applied in parallel with the NR assay in a cell viability assay, our TM test gives a concordant rank of toxic potency for the chemicals tested. In comparison with NR uptake, the TM method appears to be more accurate and the data it provides are less prone to variation.

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