Rapid method for spot visualization in thin layer chromatography

1978 ◽  
Vol 55 (1) ◽  
pp. 61
Author(s):  
Richard M. Boden
1966 ◽  
Vol 49 (2) ◽  
pp. 468-472
Author(s):  
Arthur W Davidson

Abstract Twelve collaborators from six laboratories used gas-liquid and thin layer chromatography to measure BHC, p,p′-DDT, and endrin residues on apricots and strawberries. Recoveries for both crops by gas chromatography averaged: BHC, 104 ± 23 %; p,p′-DDT, 102 ± 10%; endrin, 96 ±14%. Recoveries for both crops by thin layer chromatography averaged: BHC, 98 ± 29%; p,p′-DDT, 97 ± 18%; and endrin, 96 ± 16%.


1987 ◽  
Vol 65 (7) ◽  
pp. 668-673 ◽  
Author(s):  
Luc Ménard ◽  
Guy G. Poirier

We have developed a rapid, highly reproducible assay to determine poly(ADP-ribose) glycohydrolase activity which measures directly the appearance of the reaction product. We also analysed the majority of different techniques which are used to determine poly(ADP-ribose) glycohydrolase activity and found that the apparent activity can vary extensively depending on the method used. Thin-layer chromatography using PEI-F–cellulose was the only method which evaluated directly the specific release of ADP-ribose; by comparison with this method, the other procedures gave an over- or under-estimation of 2- to 10-fold of the enzymatic activity. A rapid method of affinity chromatography has also been developed to synthesize and purify in high yield poly(ADP-ribose) (35% conversion of 1 mM NAD to poly (ADP-ribose)).


1970 ◽  
Vol 48 (1) ◽  
pp. 49-53 ◽  
Author(s):  
Dianne Fahselt

The main anthocyanin pigments of the genus Dicentra have been characterized through the use of spectroscopy and chromatography. This combination of techniques, particularly suited to anthocyanins, has demonstrated that all are glycosides of cyanidin (3,5,7,3′,4′-pentahydroxyflavylium) and that none are acylated. The simplest, found in several species, is cyanidin-3-monoglucoside, while the most complex is cyanidin-3-rhamnoglucosido-7-xyloside. This triglycoside is present in only one species as well as in certain experimental interspecific hybrids, and this is its first reported occurrence in nature. A rapid method is described for identification of anthocyanin sugars using thin-layer chromatography.


1970 ◽  
Vol 23 (2) ◽  
pp. 127-129
Author(s):  
Tsune Tanaka ◽  
Ken-ichi Arai ◽  
Tsuneyuki Saito

Sign in / Sign up

Export Citation Format

Share Document