Ion exchange paper chromatography

1961 ◽  
Vol 38 (8) ◽  
pp. 406
Author(s):  
Alan T. Thomas ◽  
J. P. Phillips
1958 ◽  
Vol 23 (8) ◽  
pp. 1229-1230 ◽  
Author(s):  
D Myhre ◽  
F Smith

1961 ◽  
Vol 39 (2) ◽  
pp. 439-451 ◽  
Author(s):  
C. K. Harris ◽  
E. Tigane ◽  
C. S. Hanes

A method based on the use of miniature ion-exchange columns has been devised for isolating small amounts of amino acids from biological fluids and tissue extracts. The amino acids are isolated virtually free from proteins, sugars, and inorganic cations in a single treatment.Techniques are described for the preparation of solutions of the isolated amino acids for quantitative analysis by paper chromatography.


1970 ◽  
Vol 51 ◽  
pp. 556-559 ◽  
Author(s):  
M. Sinibaldi ◽  
M. Lederer

1968 ◽  
Vol 46 (3) ◽  
pp. 217-223 ◽  
Author(s):  
D. G. R. Blair

The acid-soluble metabolites of adenine-2-14C and adenine-8-14C formed by 2,6-diaminopurine-sensitive and 2,6-diaminopurine-resistant L strain mouse cells have been separated by ion-exchange resin and paper chromatography. At least 20 acid-soluble metabolites were formed in 2,6-diaminopurine-sensitive cells. Significant, but much smaller amounts of some of these metabolites were also synthesized in 2,6-diaminopurine-resistant cells.


1954 ◽  
Vol 32 (2) ◽  
pp. 94-97 ◽  
Author(s):  
L. G. Cook ◽  
K. D. Shafer

The positron emitting Na22 of 2.6 year half-life has been prepared in a nuclear reactor by the double reaction Li6(n, α)H3, Ne20(H3, n)Na22. The over-all yield was 2.3 × 10−7 atoms of Na22 per atom of tritium formed. Eleven grams of lithium as aluminum alloy turnings in a neon atmosphere at 200 p.s.i. was irradiated for 175 days in a neutron flux of about 4 × 1012 n./cm.2/sec. About 380 μc. of Na22 was obtained in a sodium extract having a specific activity of 10 mc. Na22/gm. of Na23. A specific activity in the curie/gm. level would be expected from a longer irradiation in the highest flux (6 × 1013 n./cm.2/sec.) in the NRX reactor. The separation and purification of the sodium proved practicable by paper chromatography or ion exchange; the analyses for trace Li and Na23 were carried out by radioactivation.


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