Stability Mechanisms of Laccase Isoforms using a Modified FoldX Protocol Applicable to Widely Different Proteins

2013 ◽  
Vol 9 (7) ◽  
pp. 3210-3223 ◽  
Author(s):  
Niels J. Christensen ◽  
Kasper P. Kepp
Keyword(s):  
Laccase Isoforms

Production of laccase isoforms byPleurotus pulmonarius in response to presence of phenolic and aromatic compounds

2004 ◽  
Vol 44 (2) ◽  
pp. 129-136 ◽  
Author(s):  
Cristina Giatti Marques De Souza ◽  
Giovana Kirst Tychanowicz ◽  
Daniela Farani De Souza ◽  
Rosane Marina Peralta
Keyword(s):  
Aromatic Compounds ◽  
Laccase Isoforms

Identification of Specific Laccase Isoforms Capable of Polymerizing Monolignols by an “In-Gel” Procedure

1996 ◽  
Vol 242 (1) ◽  
pp. 158-161 ◽  
Author(s):  
Raja Sterjiades ◽  
Philippe Ranocha ◽  
Alain Michel Boudet ◽  
Deborah Goffner
Keyword(s):  
Laccase Isoforms

scholarly journals Evaluation of laccases and melanization in clinical and environmental Cryptococcus neoformans samples by non-denaturing PAGE

2009 ◽  
Vol 58 (5) ◽  
pp. 563-566 ◽  
Author(s):  
Cristiane B. Pereira ◽  
Frank L. Bueno ◽  
Amanda L. T. Dias ◽  
Maísa R. P. L. Brigagão ◽  
Claudete R. Paula ◽  
...  
Keyword(s):  
Cryptococcus Neoformans ◽  
Enzyme Production ◽  
Laccase Activity ◽  
Opportunistic Pathogen ◽  
Clinical Strain ◽  
Melanin Production ◽  
Densitometry Analysis ◽  
Serotype B ◽  
Colour Quantification ◽  
Laccase Isoforms

The increased incidence of infections caused by the opportunistic pathogen Cryptococcus neoformans, which mainly affects immunocompromised patients but can also infect immunocompetent individuals, has needed additional studies on this micro-organism's pathogenicity and factors related to virulence, such as enzyme production, for a better understanding of the aetiology of cryptococcosis. The aim of this study was to verify the applicability of non-denaturing PAGE for analysis of laccases by quantification of the amount of melanin pigment produced by clinical and environmental strains of C. neoformans. After incubation of the gel with the substrate l-dopa, strains produced melanin spots of a bright brown to black colour. Quantification of these spots was performed by densitometry analysis and the amount of melanin produced was calculated and compared among the strains. All strains showed laccase activity. Serotype B strains showed a higher melanin intensity than serotype A strains. Over half of the clinical strains (56.2 %) showed the lowest melanin intensities, suggesting that melanin production may not be the main virulence factor against host defence. The clinical strain ICB 88 revealed two melanin spots on the gel, indicating the presence of two laccase isoforms. The environmental strains showed the highest values of melanin intensity, which may be related to previous exposure to environmental stress conditions.

Metabolism of hydroxylated PCB congeners by cloned laccase isoforms

2009 ◽  
Vol 82 (5) ◽  
pp. 853-860 ◽  
Author(s):  
Satoru Fujihiro ◽  
Ryusuke Higuchi ◽  
Shin Hisamatsu ◽  
Shigenori Sonoki
Keyword(s):  
Pcb Congeners ◽  
Laccase Isoforms

scholarly journals Biochemical Characteristics of Three Laccase Isoforms from the Basidiomycete Pleurotus nebrodensis

Molecules ◽  
2016 ◽  
Vol 21 (2) ◽  
pp. 203 ◽  
Author(s):  
Xianghe Yuan ◽  
Guoting Tian ◽  
Yongchang Zhao ◽  
Liyan Zhao ◽  
Hexiang Wang ◽  
...  
Keyword(s):  
Biochemical Characteristics ◽  
Pleurotus Nebrodensis ◽  
Laccase Isoforms

scholarly journals Laccase isoforms with unusual properties from the basidiomyceteSteccherinum ochraceumstrain 1833

2008 ◽  
Vol 105 (6) ◽  
pp. 2065-2075 ◽  
Author(s):  
A. Chernykh ◽  
N. Myasoedova ◽  
M. Kolomytseva ◽  
M. Ferraroni ◽  
F. Briganti ◽  
...  
Keyword(s):  
Laccase Isoforms

Production, purification and biochemical characterization of two laccase isoforms produced by Trametes versicolor grown on oak sawdust

2014 ◽  
Vol 37 (2) ◽  
pp. 391-396 ◽  
Author(s):  
Fernando Martínez-Morales ◽  
Brandt Bertrand ◽  
Angélica A. Pasión Nava ◽  
Raunel Tinoco ◽  
Lourdes Acosta-Urdapilleta ◽  
...  
Keyword(s):  
Trametes Versicolor ◽  
Biochemical Characterization ◽  
Laccase Isoforms
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